Detailed information NTG0204038

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: K08M4_RS01255
• Genome accession: NZ_CP017916
• Coordinates: 270599..271624 (+)
• Length: 341 a.a.
• NCBI ID: WP_086048617.1
• Uniprot ID: A0AA34TLL6
• Organism: Vibrio syngnathi strain K08M4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 265599..276624

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K08M4_RS01235 (K08M4_02400)	-	265926..266654 (-)	729	WP_016783699.1	glutathione peroxidase	-
K08M4_RS22445	-	266699..266878 (-)	180	WP_017099997.1	hypothetical protein	-
K08M4_RS01245 (K08M4_02410)	oxyR	266877..267770 (+)	894	WP_086048615.1	DNA-binding transcriptional regulator OxyR	-
K08M4_RS01250 (K08M4_02420)	-	267889..270447 (-)	2559	WP_198299303.1	PBP1A family penicillin-binding protein	-
K08M4_RS01255 (K08M4_02430)	pilM	270599..271624 (+)	1026	WP_086048617.1	type IV pilus assembly protein PilM	Machinery gene
K08M4_RS01260 (K08M4_02440)	pilN	271608..272222 (+)	615	WP_086048618.1	PilN domain-containing protein	Machinery gene
K08M4_RS01265 (K08M4_02450)	pilO	272215..272844 (+)	630	WP_086048619.1	type 4a pilus biogenesis protein PilO	Machinery gene
K08M4_RS01270 (K08M4_02460)	pilP	272831..273349 (+)	519	WP_086048620.1	pilus assembly protein PilP	Machinery gene
K08M4_RS01275 (K08M4_02470)	pilQ	273385..275103 (+)	1719	WP_086048621.1	type IV pilus secretin PilQ family protein	Machinery gene
K08M4_RS01280 (K08M4_02480)	aroK	275280..275798 (+)	519	WP_009848907.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38117.62 Da
• Isoelectric Point: 6.3098

>NTDB_id=204038 K08M4_RS01255 WP_086048617.1 270599..271624(+) (pilM) [Vibrio syngnathi strain K08M4]
MGSSLITGIDINHHSIKAVVLKPVGELYALAGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCCNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTTSYQVYATRKEVVDSRADALNKAGFKP
VVVDTQAHGLLNIWQLASRLYPEKKNWLLVDIGIDQTSLGIIPQDSAPFYKDIAFGTQDLRATDSPNDIEDVFGTAEDTH
KFIVNLIEKLKRQLQLYSSVNALEPISGIWLMGEGASTPMLTEELQRHFQLSCESLNPLSLFENKVAKKRRLPMDWQNFG
IAAGMAMSGLKWQGGKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=204038 K08M4_RS01255 WP_086048617.1 270599..271624(+) (pilM) [Vibrio syngnathi strain K08M4]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGCGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGTCTGCCTTTTGGCTGTTGCAACGTCGCCATTTCAGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAGAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
CCAGTCGCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGATCGACAA
CCAGTTATCAGGTGTACGCCACTCGCAAAGAAGTAGTGGATAGTCGGGCTGATGCTTTAAATAAAGCTGGGTTTAAACCT
GTTGTCGTCGACACCCAAGCACATGGGCTGCTCAATATTTGGCAGTTGGCTTCTCGGCTGTACCCAGAAAAAAAGAATTG
GCTATTGGTCGATATCGGAATCGACCAAACCTCGTTAGGGATTATCCCGCAGGATTCGGCACCCTTCTATAAAGACATCG
CCTTTGGTACTCAAGATCTTCGCGCTACTGATAGCCCTAATGACATAGAGGATGTGTTCGGCACCGCAGAGGATACACAT
AAGTTTATTGTTAATTTGATCGAAAAGCTCAAGCGCCAGTTGCAACTCTATTCCTCGGTGAATGCGCTTGAGCCGATATC
CGGGATTTGGTTGATGGGCGAGGGAGCCAGCACGCCGATGCTGACGGAAGAGTTACAAAGACATTTTCAGTTAAGTTGTG
AATCGCTGAATCCTTTGTCGTTATTTGAAAATAAGGTAGCGAAGAAACGTCGCTTACCCATGGACTGGCAAAACTTTGGA
ATTGCGGCTGGCATGGCGATGAGCGGACTCAAATGGCAAGGAGGGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	54.572	99.413	0.543
pilM	Vibrio campbellii strain DS40M4	48.83	100	0.49