Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BLL42_RS17945 Genome accession   NZ_CP017886
Coordinates   3733315..3734532 (-) Length   405 a.a.
NCBI ID   WP_071553274.1    Uniprot ID   A0A1J0EN04
Organism   Pseudomonas frederiksbergensis strain ERDD5:01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3728315..3739532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLL42_RS17905 (BLL42_17545) - 3729490..3729663 (-) 174 WP_071553267.1 DUF3094 family protein -
  BLL42_RS30510 - 3729675..3729854 (-) 180 Protein_3526 hypothetical protein -
  BLL42_RS17915 (BLL42_17550) - 3729890..3730516 (-) 627 WP_071553268.1 DUF1780 domain-containing protein -
  BLL42_RS17920 (BLL42_17555) - 3730648..3731334 (+) 687 WP_071553269.1 energy-coupling factor ABC transporter permease -
  BLL42_RS17925 (BLL42_17560) - 3731398..3731613 (+) 216 WP_071553270.1 hypothetical protein -
  BLL42_RS17930 (BLL42_17565) yacG 3731623..3731823 (-) 201 WP_071553271.1 DNA gyrase inhibitor YacG -
  BLL42_RS17935 (BLL42_17570) coaE 3731820..3732443 (-) 624 WP_071553272.1 dephospho-CoA kinase -
  BLL42_RS17940 (BLL42_17575) pilD 3732440..3733312 (-) 873 WP_071553273.1 A24 family peptidase Machinery gene
  BLL42_RS17945 (BLL42_17580) pilC 3733315..3734532 (-) 1218 WP_071553274.1 type II secretion system F family protein Machinery gene
  BLL42_RS17950 (BLL42_17585) pilB 3734536..3736236 (-) 1701 WP_071553275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BLL42_RS17955 (BLL42_17590) comP 3736462..3736878 (+) 417 WP_071553276.1 pilin Machinery gene
  BLL42_RS17960 (BLL42_17595) - 3737190..3737546 (+) 357 WP_071553277.1 BON domain-containing protein -
  BLL42_RS17965 (BLL42_17600) - 3737681..3737980 (-) 300 WP_071553278.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44222.25 Da        Isoelectric Point: 10.0557

>NTDB_id=203584 BLL42_RS17945 WP_071553274.1 3733315..3734532(-) (pilC) [Pseudomonas frederiksbergensis strain ERDD5:01]
MAIKAIKVSVYAWEGTDKKGSKITGELSGHNPALIKAQLRKQGINPGKVRKKTTSLFSVGKRIKALDIALFTRQMATMMK
ASVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ENLKAKIKKAMTYPLAVVFVAVIVTGILLVKVIPQFQSVFSGFGAKLPAFTVMVIGLSEFMQAWWWLLLGGLIVGFLGFR
HALKRSQPFRDWLDTQLLKLPIVGALLYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFAMRTCGVFPNMAIQMAAIGEESGALDDMLDKVATHYEAEVDNMVDNLTSLMEPFIMVVLGIIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=203584 BLL42_RS17945 WP_071553274.1 3733315..3734532(-) (pilC) [Pseudomonas frederiksbergensis strain ERDD5:01]
ATGGCGATCAAAGCAATCAAGGTCAGCGTGTACGCCTGGGAAGGCACCGACAAGAAAGGCAGCAAAATAACCGGCGAGTT
GAGCGGTCATAACCCGGCCTTGATCAAGGCGCAGCTACGCAAACAAGGCATCAACCCGGGCAAGGTGCGGAAAAAAACCA
CTTCACTGTTCAGTGTCGGCAAACGTATCAAGGCGCTGGACATTGCCCTCTTCACCCGGCAAATGGCGACCATGATGAAA
GCCAGTGTGCCACTGCTGCAGTCGTTCGACATCATCGGCGAGGGCTTCGACAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTCGCCACCGCGCTGCGTAAAAAGCCGCAGTACTTCGATGAGCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGGCCGGCGCTCTCGACACGCTGCTGGAGCGGGTTGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGGCCAAAATCAAAAAAGCCATGACCTATCCGCTGGCCGTGGTCTTTGTCGCAGTCATCGTCACCGGCAT
TCTGCTGGTCAAAGTGATCCCACAGTTTCAGTCGGTATTTTCCGGATTTGGTGCAAAACTCCCGGCCTTCACCGTGATGG
TGATCGGTCTGTCCGAGTTCATGCAGGCGTGGTGGTGGCTGTTGCTGGGCGGTCTGATCGTCGGGTTTCTGGGCTTTCGC
CACGCGCTAAAACGCTCACAACCCTTCCGTGACTGGCTGGACACTCAACTGCTGAAACTTCCAATCGTCGGAGCGCTGCT
CTACAAGTCGGCGGTGGCCCGTTATGCCCGCACACTGTCGACCACCTTCGCCGCTGGCGTCCCCTTGGTCGAAGCCCTGG
ATTCGGTGGCTGGCGCCACTGGCAATATCGTGTTCAAACGGGCGGTGTTGCGTATCAAACAAGATGTCTCCACCGGCATG
CAGTTAAACTTCGCGATGCGCACCTGCGGGGTTTTCCCCAACATGGCGATTCAAATGGCCGCCATTGGCGAGGAGTCCGG
CGCACTGGACGACATGCTCGACAAGGTTGCGACCCACTATGAAGCTGAAGTCGACAATATGGTCGACAACCTGACCAGCC
TGATGGAACCGTTCATCATGGTGGTGCTCGGCATCATTGTCGGCGGGCTGGTGGTTGCCATGTACCTGCCCATTTTCCAA
CTCGGCTCAGCGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0EN04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

60

100

0.607
  pilC Acinetobacter baumannii D1279779

58.416

99.753

0.583
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

42.184

99.506

0.42
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilG Neisseria meningitidis 44/76-A

41.687

99.506

0.415
  pilC Vibrio campbellii strain DS40M4

41.919

97.778

0.41
  pilC Thermus thermophilus HB27

37.5

98.765

0.37

Multiple sequence alignment