Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BLL42_RS17950 Genome accession   NZ_CP017886
Coordinates   3734536..3736236 (-) Length   566 a.a.
NCBI ID   WP_071553275.1    Uniprot ID   A0A1J0EMU9
Organism   Pseudomonas frederiksbergensis strain ERDD5:01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3729536..3741236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLL42_RS30510 - 3729675..3729854 (-) 180 Protein_3526 hypothetical protein -
  BLL42_RS17915 (BLL42_17550) - 3729890..3730516 (-) 627 WP_071553268.1 DUF1780 domain-containing protein -
  BLL42_RS17920 (BLL42_17555) - 3730648..3731334 (+) 687 WP_071553269.1 energy-coupling factor ABC transporter permease -
  BLL42_RS17925 (BLL42_17560) - 3731398..3731613 (+) 216 WP_071553270.1 hypothetical protein -
  BLL42_RS17930 (BLL42_17565) yacG 3731623..3731823 (-) 201 WP_071553271.1 DNA gyrase inhibitor YacG -
  BLL42_RS17935 (BLL42_17570) coaE 3731820..3732443 (-) 624 WP_071553272.1 dephospho-CoA kinase -
  BLL42_RS17940 (BLL42_17575) pilD 3732440..3733312 (-) 873 WP_071553273.1 A24 family peptidase Machinery gene
  BLL42_RS17945 (BLL42_17580) pilC 3733315..3734532 (-) 1218 WP_071553274.1 type II secretion system F family protein Machinery gene
  BLL42_RS17950 (BLL42_17585) pilB 3734536..3736236 (-) 1701 WP_071553275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BLL42_RS17955 (BLL42_17590) comP 3736462..3736878 (+) 417 WP_071553276.1 pilin Machinery gene
  BLL42_RS17960 (BLL42_17595) - 3737190..3737546 (+) 357 WP_071553277.1 BON domain-containing protein -
  BLL42_RS17965 (BLL42_17600) - 3737681..3737980 (-) 300 WP_071553278.1 DUF2845 domain-containing protein -
  BLL42_RS17970 (BLL42_17605) - 3737983..3740925 (-) 2943 WP_071553279.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62599.36 Da        Isoelectric Point: 5.2843

>NTDB_id=203585 BLL42_RS17950 WP_071553275.1 3734536..3736236(-) (pilB) [Pseudomonas frederiksbergensis strain ERDD5:01]
MNDIALSGLAKQLVLAELLTDQSAQQADQQAHRNRVSLVSYLVQNKLVNSRQVAEIASEHFGVAFLDLNCLDQDSQPTDL
VSEKLIRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLTTEAILVEDDKLSDAIDKFFDTHSSGMEDMAEVDLEG
LDTDAVDEQHQNAVIGQDLDDAPVVRFVNKMLLNAIKGGSSDLHFEPYEKTYRVRIRTDGILREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMPLPESKSIDFRVNTLPTLWGEKVVIRILDPASAQMGIDALGYEPDQKDLYLTALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATAVHLIIAQRLARKLCSHCKKVLAIPDEALIKEGFPR
ERIGSFTIYEPTGCDHCNNGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=203585 BLL42_RS17950 WP_071553275.1 3734536..3736236(-) (pilB) [Pseudomonas frederiksbergensis strain ERDD5:01]
ATGAATGACATCGCCCTCAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGCTCACTGACCAAAGCGCGCAACAAGC
GGATCAGCAAGCCCACCGCAATCGCGTTTCCCTGGTCAGCTATCTGGTACAAAACAAACTGGTGAACAGCCGCCAGGTCG
CGGAAATCGCCTCTGAGCATTTTGGCGTCGCCTTCCTCGACCTCAACTGTCTCGACCAAGACTCCCAGCCCACGGATCTG
GTCAGCGAAAAGCTGATTCGCCAGCACCATGCCTTGCCGCTCTGGCGGCGCGGTAACAAACTGTTCGTGGGCATTTCCGA
CCCGAGCAATCACCAGGCGATCAACGATATCCAGTTCAGCACCGGGCTCACCACCGAAGCGATCCTGGTCGAAGACGACA
AGCTCAGCGACGCCATCGACAAATTCTTCGACACCCACAGCTCGGGCATGGAAGACATGGCTGAGGTCGACCTCGAAGGT
CTGGACACCGACGCCGTCGACGAGCAGCACCAGAACGCTGTCATCGGCCAGGATTTAGACGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGTTGCTGAACGCGATCAAAGGGGGCTCCTCGGACTTGCATTTCGAACCCTACGAAAAAACCTATCGGG
TGCGGATACGTACCGATGGCATACTGCGCGAAGTCGCCAAACCGCCGATTCAACTGGCCAATCGCATCGCCGCACGCCTG
AAAGTGATGGCTAGCCTGGACATCTCGGAACGTCGCAAACCCCAGGACGGGCGGCTCAAGATGCCCCTGCCGGAAAGCAA
ATCCATCGATTTTCGGGTCAACACCCTGCCAACGTTGTGGGGCGAAAAAGTCGTCATCCGGATTCTCGACCCCGCCAGCG
CACAGATGGGCATCGACGCGCTGGGTTACGAGCCCGATCAAAAAGACCTGTACCTGACGGCGCTCAAGCAGCCTCAGGGA
CTGATTCTGGTCACCGGGCCGACGGGTTCGGGGAAAACCGTATCGCTGTACACCGGGCTGAATATCCTCAATACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCAAAGCAGG
GCCTGGACTTTGCTCAAGCGTTGCGTTCATTTCTGCGCCAGGACCCAGACGTGATCATGGTCGGCGAAATCCGCGACCTG
GAAACCGCCGAAATTGCGATCAAGGCTGCGCAAACCGGGCACCTGGTGCTTTCCACCCTGCACACCAACAGCGCGGCTGA
AACCCTGACCCGGCTGCACAACATGGGCATCCCGGGGTTCAACATCGCCACTGCCGTACACCTGATCATTGCGCAGCGCC
TGGCTCGCAAGCTATGCAGCCACTGCAAAAAAGTCCTCGCGATTCCCGACGAAGCACTGATCAAGGAAGGCTTCCCGCGC
GAACGCATCGGCTCCTTCACGATCTACGAACCCACTGGTTGCGATCACTGCAACAACGGTTACAAAGGGCGAGTCGGTAT
TTATGAAGTGGTGAAAAATACTCCGGAGCTGCAACGGCTGATCATGGCTGAAGGCAATTCTCTGGAAATCGATACGCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0EMU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Acinetobacter baylyi ADP1

57.218

100

0.574
  pilB Legionella pneumophila strain ERS1305867

56.283

99.823

0.562
  pilB Vibrio cholerae strain A1552

49.912

100

0.5
  pilB Vibrio campbellii strain DS40M4

48.404

99.647

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.365

97.35

0.481
  pilF Neisseria gonorrhoeae MS11

47.425

99.47

0.472
  pilF Thermus thermophilus HB27

36.749

100

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.941

93.463

0.364
  pilB Haemophilus influenzae Rd KW20

42.443

85.336

0.362

Multiple sequence alignment