Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BKP64_RS12915 Genome accession   NZ_CP017715
Coordinates   2773398..2774519 (-) Length   373 a.a.
NCBI ID   WP_070970735.1    Uniprot ID   A0A1D9GMX6
Organism   Marinobacter salinus strain Hb8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2768398..2779519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKP64_RS12890 (BKP64_12890) - 2768720..2769124 (-) 405 WP_070970721.1 hypothetical protein -
  BKP64_RS12895 (BKP64_12895) - 2769330..2769668 (-) 339 WP_070970724.1 DUF2782 domain-containing protein -
  BKP64_RS12900 (BKP64_12900) polA 2769756..2772482 (+) 2727 WP_070970727.1 DNA polymerase I -
  BKP64_RS12905 (BKP64_12905) - 2772721..2773095 (+) 375 WP_070970730.1 hypothetical protein -
  BKP64_RS12910 (BKP64_12910) - 2773115..2773297 (-) 183 WP_070970733.1 hypothetical protein -
  BKP64_RS12915 (BKP64_12915) pilU 2773398..2774519 (-) 1122 WP_070970735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BKP64_RS12920 (BKP64_12920) pilT 2774557..2775591 (-) 1035 WP_070970739.1 type IV pilus twitching motility protein PilT Machinery gene
  BKP64_RS12925 (BKP64_12925) - 2775733..2776437 (+) 705 WP_070970742.1 YggS family pyridoxal phosphate-dependent enzyme -
  BKP64_RS12930 (BKP64_12930) proC 2776500..2777333 (+) 834 WP_070973679.1 pyrroline-5-carboxylate reductase -
  BKP64_RS12935 (BKP64_12935) - 2777356..2777940 (+) 585 WP_070970745.1 YggT family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41573.76 Da        Isoelectric Point: 6.6619

>NTDB_id=202208 BKP64_RS12915 WP_070970735.1 2773398..2774519(-) (pilU) [Marinobacter salinus strain Hb8]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGSMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLSLPEVIKDLAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTENFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRKAVIEVLINTPLVADMIRKGEVHKLKELMSKSNESGMQTFDQA
LYRLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDRLSIQED

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=202208 BKP64_RS12915 WP_070970735.1 2773398..2774519(-) (pilU) [Marinobacter salinus strain Hb8]
ATGGAATTTGAAAAACTGCTTCGTCTAATGGTTGAGAAAGGTGGGTCCGATCTGTTCATTACCGCCGGGGTTCCCCCCAG
CATGAAAGTGAATGGCAAGGTGCTCCCGGTTACCAAAAATGCCCTGACTCCAGAGCAGACTCGCGAGTTTGTTTACGGCT
CCATGAATGACAAACAGCGGGCCGAGTTTGAGGAAACCCACGAATGCAACTTTGCGATCAGTGCCCGCGGCATTGGTCGT
TTCCGTGTGAGTGCCTTTTTCCAGCGCAACCTCTGTGGCATGGTTTTGCGCCGTATCGAAGTGAAGATTCCCCAGATAGA
CGACCTGTCGTTGCCAGAAGTCATCAAGGACCTGGCCATGACCAAGCGTGGACTTATCATGTTCGTCGGGGCGACCGGTA
CGGGCAAGTCTACCTCGCTGGCGGCAATGCTCGGGCACCGGAATCGCAACAGTCGCGGTCACATCATCTCCATCGAAGAC
CCCATCGAATTCGTGCATCAGCACCAGGGGTGTATTGTGACCCAGCGGGAAGTGGGTATTGATACCGAAAACTTCGAAGT
TGCCCTCAAAAATACGCTGCGGCAGGCGCCGGACGTTATTCTGATCGGCGAGGTTCGGACCCGCCAGACCATGGAATATT
CCGTGCAGTTTGCGGAAACGGGGCACCTGTGCCTGGCAACCCTGCACGCCAACAATGCGAACCAGGCGCTGGACCGGATC
ATCCAGTTTTTCCCGCCTGAGCAGCATAACCAGATCTGGATGGACCTCTCCCTCAACCTCAAGGCGATTGTTGCGCAACA
GCTTGTGCCAACGCCTGATGGCAAGGGACGCAAGGCGGTCATTGAAGTCCTGATCAATACGCCCCTGGTGGCAGACATGA
TTCGTAAGGGCGAGGTGCACAAGCTCAAAGAGCTGATGTCCAAATCCAACGAATCCGGCATGCAGACCTTCGATCAGGCG
CTCTACCGGCTGTATGCGGAAGGCTCTATCACCTATGAGGACGCACTCGCCCATGCCGATTCTGCCAATGACCTGCGCCT
GATGATCAAGTTGGGTGCTGACGCCCAGGGCGCGGACAAACTGTCCTCGTCGGTCGACAGGTTATCGATACAGGAGGATT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9GMX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.976

99.464

0.786
  pilU Acinetobacter baylyi ADP1

64.326

95.442

0.614
  pilU Vibrio cholerae strain A1552

56.757

99.196

0.563
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

90.885

0.383
  pilT Pseudomonas aeruginosa PAK

40.597

89.812

0.365
  pilT Acinetobacter baumannii D1279779

40.597

89.812

0.365
  pilT Acinetobacter baumannii strain A118

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.299

89.812

0.362
  pilT Acinetobacter nosocomialis M2

40.299

89.812

0.362

Multiple sequence alignment