Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   CGZ53_RS00780 Genome accession   NZ_CP022435
Coordinates   129695..130636 (+) Length   313 a.a.
NCBI ID   WP_012657673.1    Uniprot ID   B9DSZ8
Organism   Streptococcus uberis strain NZ01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 124695..135636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ53_RS00770 (CGZ53_00770) rpoC 125498..129136 (+) 3639 WP_012657671.1 DNA-directed RNA polymerase subunit beta' -
  CGZ53_RS00775 (CGZ53_00775) - 129257..129622 (+) 366 WP_100911837.1 DUF1033 family protein -
  CGZ53_RS00780 (CGZ53_00780) comGA/cglA 129695..130636 (+) 942 WP_012657673.1 competence type IV pilus ATPase ComGA Machinery gene
  CGZ53_RS00785 (CGZ53_00785) comGB 130569..131603 (+) 1035 WP_398583501.1 competence type IV pilus assembly protein ComGB Machinery gene
  CGZ53_RS00790 (CGZ53_00790) comGC/cglC 131604..131927 (+) 324 WP_012657675.1 competence type IV pilus major pilin ComGC Machinery gene
  CGZ53_RS00795 (CGZ53_00795) comGD 131881..132318 (+) 438 WP_012657676.1 competence type IV pilus minor pilin ComGD Machinery gene
  CGZ53_RS00800 (CGZ53_00800) comGE 132359..132589 (+) 231 WP_230081134.1 competence type IV pilus minor pilin ComGE -
  CGZ53_RS00805 (CGZ53_00805) comGF 132549..133004 (+) 456 WP_012657678.1 competence type IV pilus minor pilin ComGF Machinery gene
  CGZ53_RS00810 (CGZ53_00810) comGG 132982..133341 (+) 360 WP_037592916.1 competence type IV pilus minor pilin ComGG -
  CGZ53_RS00815 (CGZ53_00815) comGH 133386..134342 (+) 957 WP_046389809.1 class I SAM-dependent methyltransferase Machinery gene
  CGZ53_RS00820 (CGZ53_00820) - 134398..135588 (+) 1191 WP_012657681.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35648.93 Da        Isoelectric Point: 6.7725

>NTDB_id=200659 CGZ53_RS00780 WP_012657673.1 129695..130636(+) (comGA/cglA) [Streptococcus uberis strain NZ01]
MIQSLGMQLIQEASLKQAQDIYLIPQKDSYQVFMRVGDSRQKIGDYEWKDATNLISHFKFVAGMNVGEKRRTQLGSCDYH
IDQQGLVSLRLSTVGDYRGHESLVIRLLATQESQLHYWFDSFERIEKAIHGRGLYLFAGPVGSGKTRLMYALMEKYMQDS
QIITIEDPVEIKQDAILQLQVNNSIGMTYDNLIKLSLRHRPDLVIIGEIRDTETAQAVIRASLTGAMVFSTIHAKSIPGV
FSRLIELGVKRDELLNCLNLIAYQRLIAGGGLIDFATEKFEEHTHNQWNQEVEQLLKQGYIDNEKAKLEKIKG

Nucleotide


Download         Length: 942 bp        

>NTDB_id=200659 CGZ53_RS00780 WP_012657673.1 129695..130636(+) (comGA/cglA) [Streptococcus uberis strain NZ01]
ATGATACAAAGCTTAGGCATGCAATTGATTCAAGAAGCCAGTCTCAAACAAGCACAAGATATTTATCTCATACCTCAAAA
AGACTCTTATCAAGTTTTTATGAGAGTTGGAGATAGTCGGCAAAAAATAGGAGATTATGAGTGGAAGGATGCGACAAATC
TCATTAGTCATTTTAAATTTGTCGCAGGTATGAATGTTGGCGAGAAAAGAAGAACACAACTAGGGTCCTGTGATTATCAC
ATAGATCAGCAAGGCCTTGTCTCCTTGAGACTGTCAACAGTGGGTGACTATCGTGGGCATGAAAGTTTGGTTATCCGCCT
TTTAGCCACACAAGAGTCACAATTACATTATTGGTTTGACAGTTTTGAAAGGATTGAAAAAGCCATTCATGGTCGAGGTC
TTTATTTATTTGCTGGTCCAGTAGGTTCAGGTAAAACACGGTTGATGTACGCGTTAATGGAAAAATATATGCAGGACAGC
CAAATTATTACGATTGAAGATCCTGTTGAGATTAAGCAAGATGCTATTTTACAACTCCAAGTCAATAATAGTATTGGAAT
GACTTATGATAACCTGATTAAATTGTCTTTGAGGCATCGGCCTGATTTAGTGATTATAGGTGAAATACGTGATACAGAAA
CAGCACAGGCTGTTATTAGAGCAAGTTTGACGGGGGCCATGGTTTTCTCGACCATTCATGCTAAGAGCATTCCAGGAGTT
TTCTCTCGACTGATTGAACTCGGTGTAAAACGAGATGAATTGTTAAACTGTCTCAATTTGATAGCTTATCAACGGTTAAT
TGCAGGAGGAGGGCTAATTGATTTTGCCACAGAAAAATTCGAAGAGCATACCCATAATCAATGGAATCAAGAGGTTGAGC
AACTTCTTAAACAAGGATATATCGATAACGAAAAAGCCAAGTTGGAAAAAATTAAAGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9DSZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.023

99.361

0.626
  comGA Streptococcus mutans UA140

62.5

99.681

0.623
  comGA Streptococcus mutans UA159

62.5

99.681

0.623
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.058

99.361

0.617
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.415

99.361

0.61
  comGA Streptococcus gordonii str. Challis substr. CH1

58.654

99.681

0.585
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508