Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   CGZ53_RS00785 Genome accession   NZ_CP022435
Coordinates   130569..131603 (+) Length   344 a.a.
NCBI ID   WP_398583501.1    Uniprot ID   -
Organism   Streptococcus uberis strain NZ01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 125569..136603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ53_RS00775 (CGZ53_00775) - 129257..129622 (+) 366 WP_100911837.1 DUF1033 family protein -
  CGZ53_RS00780 (CGZ53_00780) comGA/cglA 129695..130636 (+) 942 WP_012657673.1 competence type IV pilus ATPase ComGA Machinery gene
  CGZ53_RS00785 (CGZ53_00785) comGB 130569..131603 (+) 1035 WP_398583501.1 competence type IV pilus assembly protein ComGB Machinery gene
  CGZ53_RS00790 (CGZ53_00790) comGC/cglC 131604..131927 (+) 324 WP_012657675.1 competence type IV pilus major pilin ComGC Machinery gene
  CGZ53_RS00795 (CGZ53_00795) comGD 131881..132318 (+) 438 WP_012657676.1 competence type IV pilus minor pilin ComGD Machinery gene
  CGZ53_RS00800 (CGZ53_00800) comGE 132359..132589 (+) 231 WP_230081134.1 competence type IV pilus minor pilin ComGE -
  CGZ53_RS00805 (CGZ53_00805) comGF 132549..133004 (+) 456 WP_012657678.1 competence type IV pilus minor pilin ComGF Machinery gene
  CGZ53_RS00810 (CGZ53_00810) comGG 132982..133341 (+) 360 WP_037592916.1 competence type IV pilus minor pilin ComGG -
  CGZ53_RS00815 (CGZ53_00815) comGH 133386..134342 (+) 957 WP_046389809.1 class I SAM-dependent methyltransferase Machinery gene
  CGZ53_RS00820 (CGZ53_00820) - 134398..135588 (+) 1191 WP_012657681.1 acetate kinase -
  CGZ53_RS00825 (CGZ53_00825) - 135706..135909 (+) 204 WP_012657682.1 helix-turn-helix transcriptional regulator -
  CGZ53_RS00830 (CGZ53_00830) - 135911..136363 (+) 453 WP_012657683.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39847.51 Da        Isoelectric Point: 9.5535

>NTDB_id=200660 CGZ53_RS00785 WP_398583501.1 130569..131603(+) (comGB) [Streptococcus uberis strain NZ01]
MSNFLNKDISITKKPSWKKLKAKQQYQLIKLLHNLSLSGFNLNEMVSFLEKAHLLEDHFVQRMKVDLMNGRDISEMMAHL
GFSDALVTQLSLAEVHGNTTNCLAKIEDYMAQSNRIRQKTIEVITYPMVLMTFLIVILMGLRHYLLPQIGEENQLTHFLN
LFPTVLLLTFFAMIAVVIFLRLCWRKKSQLKQLLVYSKIPLLSAVLQLYLTAYYAREWGNLIAQGVELATILQLMQKEKS
VLMREMGSTMEKALLEGKSFDQSVGIFPFFKRELSLMIEYGEIKGKLGQELDIYAQVSWENYFRKLRQMTQWLQPLIFIL
VAIIIVMIYAAMLLPMYQTIGGEI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=200660 CGZ53_RS00785 WP_398583501.1 130569..131603(+) (comGB) [Streptococcus uberis strain NZ01]
TTGAGCAACTTCTTAAACAAGGATATATCGATAACGAAAAAGCCAAGTTGGAAAAAATTAAAGGCTAAACAACAATACCA
GCTGATCAAATTATTACATAATCTTAGTCTAAGTGGGTTTAACTTGAACGAAATGGTATCTTTTCTTGAGAAGGCACATC
TCTTGGAGGATCATTTTGTTCAAAGAATGAAAGTAGATTTAATGAATGGACGAGATATTTCGGAAATGATGGCCCATCTT
GGATTCTCAGATGCATTGGTAACACAGCTAAGTTTAGCTGAAGTCCATGGGAACACCACCAACTGTTTAGCAAAGATAGA
AGATTATATGGCTCAATCCAATCGGATTAGACAAAAAACCATTGAAGTCATTACTTATCCTATGGTCTTGATGACCTTCT
TAATCGTGATTTTGATGGGCTTACGCCATTATTTACTTCCGCAAATAGGAGAGGAAAACCAGCTAACACATTTTCTAAAT
TTATTTCCAACAGTATTGCTTTTGACTTTCTTTGCTATGATTGCGGTAGTAATCTTCCTCAGACTCTGCTGGCGAAAAAA
ATCCCAGTTAAAACAATTATTAGTGTATAGTAAAATCCCTCTTTTATCAGCTGTTTTACAATTGTATCTAACTGCCTATT
ATGCGAGAGAGTGGGGCAATCTTATTGCACAAGGTGTTGAACTAGCAACTATTTTGCAACTGATGCAAAAGGAAAAATCC
GTATTGATGCGGGAAATGGGTTCAACAATGGAAAAAGCTTTGTTGGAAGGGAAATCTTTTGACCAGTCTGTGGGCATCTT
CCCATTTTTTAAAAGGGAATTGAGCTTAATGATTGAATATGGAGAAATCAAAGGAAAACTCGGTCAAGAGTTAGACATTT
ATGCACAAGTCAGTTGGGAAAACTATTTTAGAAAGTTACGACAGATGACTCAATGGTTACAACCCCTCATTTTCATACTT
GTAGCCATTATAATCGTTATGATTTATGCGGCAATGTTATTGCCAATGTATCAAACCATCGGAGGTGAAATATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(31-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA159

53.254

98.256

0.523
  comGB Streptococcus mutans UA140

53.254

98.256

0.523
  comGB Streptococcus gordonii str. Challis substr. CH1

47.353

98.837

0.468
  comGB/cglB Streptococcus mitis NCTC 12261

47.305

97.093

0.459
  comGB Lactococcus lactis subsp. cremoris KW2

45.161

99.128

0.448
  comGB/cglB Streptococcus mitis SK321

45.946

96.802

0.445
  comGB/cglB Streptococcus pneumoniae Rx1

45.045

96.802

0.436
  comGB/cglB Streptococcus pneumoniae D39

45.045

96.802

0.436
  comGB/cglB Streptococcus pneumoniae R6

45.045

96.802

0.436
  comGB/cglB Streptococcus pneumoniae TIGR4

45.045

96.802

0.436