Detailed information NTG0200663

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CGZ53_RS00805
• Genome accession: NZ_CP022435
• Coordinates: 132549..133004 (+)
• Length: 151 a.a.
• NCBI ID: WP_012657678.1
• Uniprot ID: B9DT03
• Organism: Streptococcus uberis strain NZ01
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 127549..138004

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CGZ53_RS00775 (CGZ53_00775)	-	129257..129622 (+)	366	WP_100911837.1	DUF1033 family protein	-
CGZ53_RS00780 (CGZ53_00780)	comGA/cglA	129695..130636 (+)	942	WP_012657673.1	competence type IV pilus ATPase ComGA	Machinery gene
CGZ53_RS00785 (CGZ53_00785)	comGB	130569..131603 (+)	1035	WP_398583501.1	competence type IV pilus assembly protein ComGB	Machinery gene
CGZ53_RS00790 (CGZ53_00790)	comGC/cglC	131604..131927 (+)	324	WP_012657675.1	competence type IV pilus major pilin ComGC	Machinery gene
CGZ53_RS00795 (CGZ53_00795)	comGD	131881..132318 (+)	438	WP_012657676.1	competence type IV pilus minor pilin ComGD	Machinery gene
CGZ53_RS00800 (CGZ53_00800)	comGE	132359..132589 (+)	231	WP_230081134.1	competence type IV pilus minor pilin ComGE	-
CGZ53_RS00805 (CGZ53_00805)	comGF	132549..133004 (+)	456	WP_012657678.1	competence type IV pilus minor pilin ComGF	Machinery gene
CGZ53_RS00810 (CGZ53_00810)	comGG	132982..133341 (+)	360	WP_037592916.1	competence type IV pilus minor pilin ComGG	-
CGZ53_RS00815 (CGZ53_00815)	comGH	133386..134342 (+)	957	WP_046389809.1	class I SAM-dependent methyltransferase	Machinery gene
CGZ53_RS00820 (CGZ53_00820)	-	134398..135588 (+)	1191	WP_012657681.1	acetate kinase	-
CGZ53_RS00825 (CGZ53_00825)	-	135706..135909 (+)	204	WP_012657682.1	helix-turn-helix transcriptional regulator	-
CGZ53_RS00830 (CGZ53_00830)	-	135911..136363 (+)	453	WP_012657683.1	hypothetical protein	-
CGZ53_RS00835 (CGZ53_00835)	-	136372..137025 (+)	654	WP_012657684.1	CPBP family intramembrane glutamic endopeptidase	-
CGZ53_RS00840 (CGZ53_00840)	proC	137051..137821 (-)	771	WP_100911838.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 151 a.a.
• Molecular weight: 17647.48 Da
• Isoelectric Point: 10.0997

>NTDB_id=200663 CGZ53_RS00805 WP_012657678.1 132549..133004(+) (comGF) [Streptococcus uberis strain NZ01]
MIKKYSKLVACPTKAFTLLEALVALLVISGSLLLYQGLTKSIFSQMTYLRENNQDRWLLFSHQLRAELMGSRFQKVEKNK
IYVTKDGKDVAYGLSKRDDFRKTSANGHGYHPMLLHLSQATIEEKERTVTIHFVWKDGLERTWLYDFKENR

Nucleotide

• Length: 456 bp

>NTDB_id=200663 CGZ53_RS00805 WP_012657678.1 132549..133004(+) (comGF) [Streptococcus uberis strain NZ01]
ATGATCAAGAAATACTCAAAATTAGTCGCTTGTCCAACTAAAGCCTTTACACTTTTGGAAGCTCTAGTAGCCTTATTAGT
AATATCAGGCTCGCTGTTACTCTATCAAGGCCTGACTAAATCTATTTTTTCTCAGATGACTTATTTAAGAGAAAACAATC
AGGATAGATGGTTACTCTTTTCTCATCAGTTGCGAGCGGAGTTAATGGGATCTCGTTTTCAGAAAGTTGAAAAGAATAAA
ATTTATGTGACCAAGGATGGTAAGGATGTAGCATATGGGCTTTCAAAAAGGGACGACTTTCGCAAAACATCGGCAAATGG
TCATGGCTATCATCCCATGTTATTACATTTAAGTCAGGCCACAATTGAAGAAAAAGAAAGAACTGTCACCATTCATTTTG
TTTGGAAGGATGGTCTGGAGAGGACATGGCTATATGATTTTAAAGAAAACCGTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-140)

3D structure

• AlphaFold DB: B9DT03

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA140	50	90.066	0.45
comGF/cglF	Streptococcus mitis NCTC 12261	51.515	87.417	0.45
comGF	Streptococcus mutans UA159	49.265	90.066	0.444
comGF/cglF	Streptococcus pneumoniae D39	50.376	88.079	0.444
comGF/cglF	Streptococcus pneumoniae TIGR4	50.376	88.079	0.444
comGF/cglF	Streptococcus pneumoniae R6	50.376	88.079	0.444
comGF/cglF	Streptococcus mitis SK321	50.376	88.079	0.444
comGF/cglF	Streptococcus pneumoniae Rx1	50.376	88.079	0.444
comGF	Lactococcus lactis subsp. cremoris KW2	43.478	91.391	0.397