Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BI364_RS14400 Genome accession   NZ_CP017415
Coordinates   2977870..2978340 (+) Length   156 a.a.
NCBI ID   WP_070079337.1    Uniprot ID   A0A1D8IRI0
Organism   Acidihalobacter yilgarnensis strain F5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2972870..2983340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI364_RS14380 (BI364_14380) - 2973849..2974079 (+) 231 WP_070080101.1 PP0621 family protein -
  BI364_RS14385 (BI364_14385) - 2974083..2975510 (+) 1428 WP_070079335.1 nitrogen regulation protein NR(II) -
  BI364_RS14390 (BI364_14390) pilR 2975612..2976967 (+) 1356 WP_070079336.1 sigma-54 dependent transcriptional regulator Regulator
  BI364_RS19010 (BI364_14395) pilA2 2977153..2977431 (+) 279 WP_083251559.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  BI364_RS17625 - 2977365..2977649 (+) 285 WP_197495732.1 pilin -
  BI364_RS14400 (BI364_14400) pilA 2977870..2978340 (+) 471 WP_070079337.1 pilin Machinery gene
  BI364_RS14405 (BI364_14405) - 2978411..2980399 (+) 1989 WP_070079338.1 hypothetical protein -
  BI364_RS14410 (BI364_14410) - 2980392..2981267 (+) 876 WP_070079339.1 glycosyltransferase family 2 protein -
  BI364_RS14415 (BI364_14415) - 2981264..2982418 (+) 1155 WP_070079340.1 glycosyltransferase family 4 protein -
  BI364_RS14420 (BI364_14420) - 2982415..2983155 (+) 741 WP_070079341.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16269.75 Da        Isoelectric Point: 9.3384

>NTDB_id=197996 BI364_RS14400 WP_070079337.1 2977870..2978340(+) (pilA) [Acidihalobacter yilgarnensis strain F5]
MSEQRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAKVTEGLNLAGAAKSAIWDTYGNLGTFPTVGGNASYGLPATI
AGTNTSNVTIGSQGLITITYKNNLGGAANGKTLLLQPWTHGGVIEWICYAANKPAQTPLSTNTATLPGKYAPAICR

Nucleotide


Download         Length: 471 bp        

>NTDB_id=197996 BI364_RS14400 WP_070079337.1 2977870..2978340(+) (pilA) [Acidihalobacter yilgarnensis strain F5]
ATGAGCGAACAACGACAAAAAGGCTTCACCCTCATCGAACTGATGATCGTAGTGGCCATCATCGGCATTCTGGCCGCCAT
CGCCATTCCTGCTTACCAGGACTACACCATCCGCGCCAAGGTGACGGAGGGCCTCAATCTCGCCGGCGCGGCCAAATCGG
CCATCTGGGACACCTACGGCAACCTCGGCACTTTCCCCACAGTCGGCGGCAACGCAAGCTATGGGCTCCCCGCGACGATT
GCCGGCACCAACACCAGTAACGTCACCATCGGCTCGCAAGGCCTGATCACCATCACCTACAAAAACAATCTTGGCGGCGC
AGCCAATGGCAAAACCCTGCTGTTGCAGCCTTGGACGCACGGCGGCGTGATCGAATGGATCTGCTACGCGGCAAACAAGC
CCGCGCAAACACCCTTGAGCACCAACACAGCCACCCTGCCCGGAAAATACGCGCCTGCGATCTGCCGCTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-156)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8IRI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

47.093

100

0.519
  pilA2 Legionella pneumophila strain ERS1305867

49.673

98.077

0.487
  pilA2 Legionella pneumophila str. Paris

49.673

98.077

0.487
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.442
  comP Acinetobacter baylyi ADP1

41.25

100

0.423
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.415

100

0.404
  pilE Neisseria gonorrhoeae MS11

39.241

100

0.397
  pilE Neisseria gonorrhoeae strain FA1090

38.462

100

0.385
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.312

98.718

0.378

Multiple sequence alignment