Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   Q5O_RS24945 Genome accession   NZ_CP016212
Coordinates   5491028..5492290 (-) Length   420 a.a.
NCBI ID   WP_019439130.1    Uniprot ID   -
Organism   Pseudomonas putida JB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5486028..5497290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5O_RS24930 (Q5O_24930) - 5487706..5489295 (-) 1590 WP_019439128.1 SPOR domain-containing protein -
  Q5O_RS24935 (Q5O_24935) aroB 5489311..5490408 (-) 1098 WP_041166613.1 3-dehydroquinate synthase -
  Q5O_RS24940 (Q5O_24940) aroK 5490505..5491023 (-) 519 WP_010955625.1 shikimate kinase AroK -
  Q5O_RS24945 (Q5O_24945) pilQ 5491028..5492290 (-) 1263 WP_019439130.1 type IV pilus secretin PilQ Machinery gene
  Q5O_RS24950 (Q5O_24950) - 5492214..5493215 (-) 1002 WP_014592711.1 pilus assembly protein PilP -
  Q5O_RS24955 (Q5O_24955) - 5493227..5493763 (-) 537 WP_010955628.1 PilN domain-containing protein -
  Q5O_RS24960 (Q5O_24960) pilM 5493760..5494641 (-) 882 WP_019439131.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45699.41 Da        Isoelectric Point: 4.9883

>NTDB_id=186222 Q5O_RS24945 WP_019439130.1 5491028..5492290(-) (pilQ) [Pseudomonas putida JB]
MKTWMNYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVKGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQALDAHLQPLRRELLPIHHAKAADLAQLLLAGLEGDGIPPASLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=186222 Q5O_RS24945 WP_019439130.1 5491028..5492290(-) (pilQ) [Pseudomonas putida JB]
ATGAAAACGTGGATGAACTACCTGTTGGCAACCCTGCTGTCGATACCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTTGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCTGGCGTCAACCTGGTCG
CCAGTGATGCAGTAAAAGGCAGTGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCACCTGCAGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGTCAGGCGCTGGATGCGCACCTGCAGCCATTGCGTCGAGAGCTGCTGCCCATTCACCACGCCAAGGCCG
CCGATCTTGCGCAGTTGCTGCTGGCGGGCCTGGAGGGTGACGGCATCCCTCCGGCTAGCTTGAGTGTCGATGAGCGCACC
AATACGTTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGCTGGTGGCGCAGTTGGATGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAAGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGTGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCGAGGATACTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACCCCGGACAACAAGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTGCGCGTGGCTGATGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.614
  comQ Acinetobacter baylyi ADP1

42.316

100

0.452
  pilQ Acinetobacter baumannii strain A118

40.998

100

0.45
  pilQ Acinetobacter baumannii D1279779

40.998

100

0.45
  pilQ Legionella pneumophila strain ERS1305867

42.788

99.048

0.424
  pilQ Legionella pneumophila strain Lp02

42.788

99.048

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.943

100

0.421
  pilQ Vibrio cholerae strain A1552

41.943

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.46

100

0.419
  pilQ Neisseria gonorrhoeae strain FA1090

35.765

100

0.362
  pilQ Neisseria meningitidis MC58

35.765

100

0.362
  pilQ Neisseria meningitidis M1080

35.765

100

0.362

Multiple sequence alignment