Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLJM4_RS11495 Genome accession   NZ_CP015909
Coordinates   2228077..2229057 (-) Length   326 a.a.
NCBI ID   WP_162494683.1    Uniprot ID   -
Organism   Lactococcus cremoris strain JM4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2223077..2234057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM4_RS11450 (LLJM4_2229) - 2223095..2223832 (-) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLJM4_RS11455 (LLJM4_2230) - 2224011..2224853 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLJM4_RS11460 (LLJM4_2231) - 2224850..2225287 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLJM4_RS11465 (LLJM4_04445) comGG 2225367..2225594 (-) 228 WP_228764408.1 competence protein ComGG Machinery gene
  LLJM4_RS11470 (LLJM4_2233) comGF 2225690..2226115 (-) 426 WP_375339262.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLJM4_RS11475 (LLJM4_2234) comGE 2226099..2226335 (-) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLJM4_RS11480 (LLJM4_2235) comGD 2226367..2226555 (-) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLJM4_RS11485 (LLJM4_2236) comGC 2226757..2227134 (-) 378 Protein_2238 competence type IV pilus major pilin ComGC -
  LLJM4_RS11490 (LLJM4_2237) comGB 2227152..2228177 (-) 1026 WP_050574185.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLJM4_RS11495 (LLJM4_2238) comGA 2228077..2229057 (-) 981 WP_162494683.1 competence type IV pilus ATPase ComGA Machinery gene
  LLJM4_RS11500 (LLJM4_2239) - 2229172..2232954 (-) 3783 Protein_2241 PolC-type DNA polymerase III -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 37019.32 Da        Isoelectric Point: 5.9464

>NTDB_id=183434 LLJM4_RS11495 WP_162494683.1 2228077..2229057(-) (comGA) [Lactococcus cremoris strain JM4]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=183434 LLJM4_RS11495 WP_162494683.1 2228077..2229057(-) (comGA) [Lactococcus cremoris strain JM4]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

99.08

100

0.991
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.452

95.092

0.537
  comYA Streptococcus gordonii str. Challis substr. CH1

54.43

96.933

0.528
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.487

95.706

0.521
  comYA Streptococcus mutans UA159

52.733

95.399

0.503
  comYA Streptococcus mutans UA140

52.733

95.399

0.503

Multiple sequence alignment