Detailed information NTG0183430

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLJM4_RS11470
• Genome accession: NZ_CP015909
• Coordinates: 2225690..2226115 (-)
• Length: 141 a.a.
• NCBI ID: WP_375339262.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain JM4
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2220690..2231115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJM4_RS11440 (LLJM4_04440)	-	2220826..2222108 (-)	1283	Protein_2229	IS3 family transposase	-
LLJM4_RS11445 (LLJM4_2228)	-	2222293..2223102 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLJM4_RS11450 (LLJM4_2229)	-	2223095..2223832 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLJM4_RS11455 (LLJM4_2230)	-	2224011..2224853 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLJM4_RS11460 (LLJM4_2231)	-	2224850..2225287 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLJM4_RS11465 (LLJM4_04445)	comGG	2225367..2225594 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLJM4_RS11470 (LLJM4_2233)	comGF	2225690..2226115 (-)	426	WP_375339262.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLJM4_RS11475 (LLJM4_2234)	comGE	2226099..2226335 (-)	237	WP_014573335.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLJM4_RS11480 (LLJM4_2235)	comGD	2226367..2226555 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLJM4_RS11485 (LLJM4_2236)	comGC	2226757..2227134 (-)	378	Protein_2238	competence type IV pilus major pilin ComGC	-
LLJM4_RS11490 (LLJM4_2237)	comGB	2227152..2228177 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLJM4_RS11495 (LLJM4_2238)	comGA	2228077..2229057 (-)	981	WP_162494683.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16111.64 Da
• Isoelectric Point: 8.4549

>NTDB_id=183430 LLJM4_RS11470 WP_375339262.1 2225690..2226115(-) (comGF) [Lactococcus cremoris strain JM4]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDSQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=183430 LLJM4_RS11470 WP_375339262.1 2225690..2226115(-) (comGF) [Lactococcus cremoris strain JM4]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTCGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	99.291	100	0.993
comYF	Streptococcus mutans UA140	43.262	100	0.433
comYF	Streptococcus mutans UA159	42.553	100	0.426
comGF/cglF	Streptococcus mitis NCTC 12261	38.571	99.291	0.383
comGF/cglF	Streptococcus mitis SK321	38.571	99.291	0.383
comGF/cglF	Streptococcus pneumoniae Rx1	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae D39	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae R6	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae TIGR4	37.143	99.291	0.369