Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   LLJM3_RS10370 Genome accession   NZ_CP015901
Coordinates   2020847..2021272 (-) Length   141 a.a.
NCBI ID   WP_011676939.1    Uniprot ID   A0AA34TL31
Organism   Lactococcus cremoris strain JM3     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1985619..2031174 2020847..2021272 within 0


Gene organization within MGE regions


Location: 1985619..2031174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM3_RS10140 (LLJM3_1995) - 1987464..1988240 (-) 777 WP_011676898.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LLJM3_RS10145 (LLJM3_1996) rsmB 1988363..1989637 (-) 1275 WP_011676899.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LLJM3_RS10150 (LLJM3_1997) - 1989925..1990551 (-) 627 WP_011676900.1 hypothetical protein -
  LLJM3_RS10155 (LLJM3_1998) - 1990662..1991600 (-) 939 WP_041167469.1 glycosyltransferase family 2 protein -
  LLJM3_RS10160 (LLJM3_1999) - 1991518..1993119 (-) 1602 WP_011676902.1 glycosyltransferase family 39 protein -
  LLJM3_RS10165 (LLJM3_2000) - 1993259..1994038 (-) 780 WP_063284079.1 peptidoglycan amidohydrolase family protein -
  LLJM3_RS10170 (LLJM3_2001) - 1994025..1994327 (-) 303 WP_063284078.1 phage holin -
  LLJM3_RS10175 (LLJM3_2002) - 1994340..1994711 (-) 372 WP_011676904.1 hypothetical protein -
  LLJM3_RS13915 - 1994729..1995073 (-) 345 WP_371857207.1 hypothetical protein -
  LLJM3_RS13920 - 1995266..1995847 (-) 582 Protein_1985 hypothetical protein -
  LLJM3_RS10185 (LLJM3_2004) - 1996186..1996323 (-) 138 WP_011676905.1 hypothetical protein -
  LLJM3_RS10190 (LLJM3_2005) - 1996320..1998821 (-) 2502 WP_041167470.1 gp58-like family protein -
  LLJM3_RS10195 (LLJM3_2006) - 1998833..1999198 (-) 366 WP_011676906.1 DUF6711 family protein -
  LLJM3_RS10200 (LLJM3_2007) - 1999210..2003910 (-) 4701 WP_011676907.1 tail protein -
  LLJM3_RS10205 (LLJM3_2008) - 2003942..2004313 (-) 372 WP_011676908.1 hypothetical protein -
  LLJM3_RS10210 (LLJM3_2009) - 2004337..2004747 (-) 411 WP_011676909.1 DUF6096 family protein -
  LLJM3_RS10215 (LLJM3_2010) - 2004821..2005234 (-) 414 WP_011676910.1 phage tail tube protein -
  LLJM3_RS10220 (LLJM3_2011) - 2005247..2005609 (-) 363 WP_011676911.1 hypothetical protein -
  LLJM3_RS10225 (LLJM3_2012) - 2005609..2006157 (-) 549 WP_011676912.1 hypothetical protein -
  LLJM3_RS10230 (LLJM3_2013) - 2006141..2006494 (-) 354 WP_041167471.1 hypothetical protein -
  LLJM3_RS10235 (LLJM3_2014) - 2006475..2006801 (-) 327 WP_011676914.1 phage head-tail connector protein -
  LLJM3_RS10240 (LLJM3_2015) - 2006822..2007940 (-) 1119 WP_011676915.1 Ig-like domain-containing protein -
  LLJM3_RS10245 (LLJM3_2016) - 2007952..2008608 (-) 657 WP_041167616.1 DUF4355 domain-containing protein -
  LLJM3_RS10250 (LLJM3_2017) - 2008773..2009879 (-) 1107 WP_011676917.1 minor capsid protein -
  LLJM3_RS10255 (LLJM3_2018) - 2009885..2010172 (-) 288 WP_011676918.1 ribosomal-processing cysteine protease Prp -
  LLJM3_RS10260 (LLJM3_2019) - 2010169..2010399 (-) 231 WP_041167618.1 hypothetical protein -
  LLJM3_RS10265 (LLJM3_2020) - 2010441..2010605 (-) 165 WP_011676920.1 hypothetical protein -
  LLJM3_RS10270 (LLJM3_2021) - 2010562..2012070 (-) 1509 WP_011676921.1 phage portal protein -
  LLJM3_RS10275 (LLJM3_2022) - 2012079..2013314 (-) 1236 WP_011676922.1 PBSX family phage terminase large subunit -
  LLJM3_RS10280 (LLJM3_2023) - 2013304..2013816 (-) 513 WP_011676923.1 terminase small subunit -
  LLJM3_RS10285 (LLJM3_2024) - 2014112..2014537 (-) 426 WP_003131928.1 RinA family protein -
  LLJM3_RS10290 (LLJM3_2025) - 2014614..2014922 (-) 309 WP_011676924.1 hypothetical protein -
  LLJM3_RS10295 (LLJM3_2026) - 2015255..2015467 (-) 213 WP_237024555.1 hypothetical protein -
  LLJM3_RS10300 (LLJM3_2027) - 2015448..2015642 (-) 195 WP_011676926.1 DUF1660 domain-containing protein -
  LLJM3_RS10305 (LLJM3_2028) - 2015639..2015881 (-) 243 WP_011676927.1 hypothetical protein -
  LLJM3_RS10310 (LLJM3_2029) - 2015932..2016618 (-) 687 WP_011676928.1 hypothetical protein -
  LLJM3_RS10315 (LLJM3_2030) - 2016605..2016916 (-) 312 WP_011676929.1 hypothetical protein -
  LLJM3_RS10320 (LLJM3_2031) dut 2016916..2017335 (-) 420 WP_011676930.1 dUTP diphosphatase -
  LLJM3_RS10325 (LLJM3_2032) - 2017332..2017688 (-) 357 WP_011676931.1 hypothetical protein -
  LLJM3_RS10330 (LLJM3_2033) - 2017685..2018206 (-) 522 WP_011676932.1 DUF1642 domain-containing protein -
  LLJM3_RS10335 (LLJM3_2034) - 2018199..2018405 (-) 207 WP_011676933.1 DUF1125 domain-containing protein -
  LLJM3_RS10340 (LLJM3_2035) - 2018419..2018943 (-) 525 WP_011676934.1 hypothetical protein -
  LLJM3_RS10345 (LLJM3_2036) - 2019050..2019289 (-) 240 WP_011676935.1 DUF1031 domain-containing protein -
  LLJM3_RS10350 (LLJM3_2037) - 2019290..2019421 (-) 132 WP_257590451.1 hypothetical protein -
  LLJM3_RS10355 (LLJM3_2038) - 2019456..2019710 (-) 255 WP_011676936.1 hypothetical protein -
  LLJM3_RS10360 (LLJM3_2039) - 2019700..2019921 (-) 222 WP_011676937.1 hypothetical protein -
  LLJM3_RS13835 (LLJM3_04720) - 2019902..2020704 (-) 803 Protein_2022 DnaD domain protein -
  LLJM3_RS10370 (LLJM3_2041) ssb 2020847..2021272 (-) 426 WP_011676939.1 single-stranded DNA-binding protein Machinery gene
  LLJM3_RS10375 (LLJM3_2042) - 2021265..2022023 (-) 759 WP_011676940.1 Rad52/Rad22 family DNA repair protein -
  LLJM3_RS10380 (LLJM3_2043) - 2022032..2022427 (-) 396 WP_011676941.1 hypothetical protein -
  LLJM3_RS10385 (LLJM3_2044) - 2022526..2022762 (-) 237 WP_011676942.1 DUF1408 domain-containing protein -
  LLJM3_RS10390 (LLJM3_2045) - 2022868..2023224 (+) 357 WP_011676943.1 DUF2513 domain-containing protein -
  LLJM3_RS10395 (LLJM3_2046) - 2023212..2023646 (-) 435 WP_011676944.1 hypothetical protein -
  LLJM3_RS10400 (LLJM3_2047) - 2023740..2024009 (-) 270 WP_011676945.1 hypothetical protein -
  LLJM3_RS10405 (LLJM3_2048) - 2024022..2024804 (-) 783 WP_011676946.1 phage repressor protein/antirepressor Ant -
  LLJM3_RS10410 (LLJM3_2049) - 2024817..2025056 (-) 240 WP_011676947.1 helix-turn-helix transcriptional regulator -
  LLJM3_RS10415 (LLJM3_2050) - 2025154..2025663 (+) 510 WP_011676948.1 hypothetical protein -
  LLJM3_RS10420 (LLJM3_2052) - 2026281..2026475 (-) 195 WP_011676952.1 putative transcriptional regulator -
  LLJM3_RS10425 (LLJM3_2053) - 2026738..2027265 (+) 528 WP_011676953.1 helix-turn-helix domain-containing protein -
  LLJM3_RS10430 (LLJM3_2054) - 2027271..2027705 (+) 435 WP_011676954.1 zinc peptidase -
  LLJM3_RS10435 (LLJM3_2055) - 2027719..2028054 (+) 336 WP_011676955.1 hypothetical protein -
  LLJM3_RS10440 (LLJM3_2056) - 2028121..2029302 (+) 1182 WP_011676956.1 site-specific integrase -
  LLJM3_RS10445 (LLJM3_2057) - 2029597..2030103 (-) 507 WP_011676957.1 Asp23/Gls24 family envelope stress response protein -
  LLJM3_RS10450 (LLJM3_2058) - 2030113..2030301 (-) 189 WP_011676958.1 DUF2273 domain-containing protein -
  LLJM3_RS10455 (LLJM3_2059) amaP 2030312..2030872 (-) 561 WP_011676959.1 alkaline shock response membrane anchor protein AmaP -
  LLJM3_RS10460 (LLJM3_2060) - 2030900..2031142 (-) 243 WP_011676960.1 GlsB/YeaQ/YmgE family stress response membrane protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15693.35 Da        Isoelectric Point: 5.1972

>NTDB_id=183034 LLJM3_RS10370 WP_011676939.1 2020847..2021272(-) (ssb) [Lactococcus cremoris strain JM3]
MINNVTLVGRITKEPELRYTQQNKAVASFTLAVNRAFKNANGEREADFINCVIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENQQGQRVYITEVVASNFQVLEKSNQANGERVSNPASKPQNNDSFGSDPMEVSDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=183034 LLJM3_RS10370 WP_011676939.1 2020847..2021272(-) (ssb) [Lactococcus cremoris strain JM3]
ATGATTAACAATGTCACTCTAGTAGGAAGAATCACTAAAGAACCTGAACTTAGATATACACAACAAAATAAAGCAGTTGC
TTCATTTACTCTTGCAGTTAATCGAGCATTTAAAAATGCTAATGGAGAAAGAGAAGCTGACTTTATCAATTGTGTTATTT
GGGGTAAATCAGCCGAAAACTTGGCCAATTGGACTCATAAAGGTCAATTAATTGGAGTTACTGGTAGTATTCAAACTCGA
AACTATGAGAACCAACAAGGGCAGCGTGTTTATATTACGGAGGTTGTCGCAAGTAATTTCCAAGTACTAGAAAAAAGTAA
TCAAGCAAATGGTGAACGAGTTAGTAATCCAGCTTCAAAACCACAAAATAACGATTCTTTTGGAAGTGATCCAATGGAAG
TTTCAGATGATGACCTACCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

52.353

100

0.631
  ssbA Bacillus subtilis subsp. subtilis str. 168

50.581

100

0.617
  ssbB/cilA Streptococcus mitis NCTC 12261

43.262

100

0.433
  ssbB/cilA Streptococcus pneumoniae TIGR4

43.262

100

0.433
  ssbB/cilA Streptococcus pneumoniae Rx1

42.553

100

0.426
  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.553

100

0.426
  ssbB/cilA Streptococcus pneumoniae D39

42.553

100

0.426
  ssbB/cilA Streptococcus pneumoniae R6

42.553

100

0.426
  ssbB/cilA Streptococcus mitis SK321

42.553

100

0.426
  ssbB Bacillus subtilis subsp. subtilis str. 168

57.692

73.759

0.426
  ssbA Streptococcus mutans UA159

39.007

100

0.39
  ssbB Lactococcus lactis subsp. cremoris KW2

50.485

73.05

0.369

Multiple sequence alignment