Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLJM2_RS11225 Genome accession   NZ_CP015900
Coordinates   2169465..2170445 (-) Length   326 a.a.
NCBI ID   WP_162494683.1    Uniprot ID   -
Organism   Lactococcus cremoris strain JM2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2162146..2170376 2169465..2170445 flank -911


Gene organization within MGE regions


Location: 2162146..2170445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM2_RS11175 (LLJM2_2193) - 2163681..2164490 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLJM2_RS11180 (LLJM2_2194) - 2164483..2165220 (-) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLJM2_RS11185 (LLJM2_2195) - 2165399..2166241 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLJM2_RS11190 (LLJM2_2196) - 2166238..2166675 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLJM2_RS11195 (LLJM2_03575) comGG 2166755..2166982 (-) 228 WP_228764408.1 competence protein ComGG Machinery gene
  LLJM2_RS11200 (LLJM2_2198) comGF 2167078..2167503 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLJM2_RS11205 (LLJM2_2199) comGE 2167487..2167723 (-) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLJM2_RS11210 (LLJM2_2200) comGD 2167755..2167943 (-) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLJM2_RS11215 (LLJM2_2201) comGC 2168145..2168522 (-) 378 Protein_2171 competence type IV pilus major pilin ComGC -
  LLJM2_RS11220 (LLJM2_2202) comGB 2168540..2169565 (-) 1026 WP_050574185.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLJM2_RS11225 (LLJM2_2203) comGA 2169465..2170445 (-) 981 WP_162494683.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 37019.32 Da        Isoelectric Point: 5.9464

>NTDB_id=183000 LLJM2_RS11225 WP_162494683.1 2169465..2170445(-) (comGA) [Lactococcus cremoris strain JM2]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=183000 LLJM2_RS11225 WP_162494683.1 2169465..2170445(-) (comGA) [Lactococcus cremoris strain JM2]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

99.08

100

0.991
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.452

95.092

0.537
  comYA Streptococcus gordonii str. Challis substr. CH1

54.43

96.933

0.528
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.487

95.706

0.521
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.487

95.706

0.521
  comYA Streptococcus mutans UA159

52.733

95.399

0.503
  comYA Streptococcus mutans UA140

52.733

95.399

0.503

Multiple sequence alignment