Detailed information NTG0182996

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLJM2_RS11200
• Genome accession: NZ_CP015900
• Coordinates: 2167078..2167503 (-)
• Length: 141 a.a.
• NCBI ID: WP_014735174.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain JM2
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2162146..2170376	2167078..2167503	within	0

Gene organization within MGE regions

Location: 2162146..2170376

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJM2_RS11175 (LLJM2_2193)	-	2163681..2164490 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLJM2_RS11180 (LLJM2_2194)	-	2164483..2165220 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLJM2_RS11185 (LLJM2_2195)	-	2165399..2166241 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLJM2_RS11190 (LLJM2_2196)	-	2166238..2166675 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLJM2_RS11195 (LLJM2_03575)	comGG	2166755..2166982 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLJM2_RS11200 (LLJM2_2198)	comGF	2167078..2167503 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLJM2_RS11205 (LLJM2_2199)	comGE	2167487..2167723 (-)	237	WP_014573335.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLJM2_RS11210 (LLJM2_2200)	comGD	2167755..2167943 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLJM2_RS11215 (LLJM2_2201)	comGC	2168145..2168522 (-)	378	Protein_2171	competence type IV pilus major pilin ComGC	-
LLJM2_RS11220 (LLJM2_2202)	comGB	2168540..2169565 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16210.78 Da
• Isoelectric Point: 8.4549

>NTDB_id=182996 LLJM2_RS11200 WP_014735174.1 2167078..2167503(-) (comGF) [Lactococcus cremoris strain JM2]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=182996 LLJM2_RS11200 WP_014735174.1 2167078..2167503(-) (comGF) [Lactococcus cremoris strain JM2]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	100	100	1
comYF	Streptococcus mutans UA140	43.972	100	0.44
comYF	Streptococcus mutans UA159	43.262	100	0.433
comGF/cglF	Streptococcus mitis NCTC 12261	39.286	99.291	0.39
comGF/cglF	Streptococcus mitis SK321	39.286	99.291	0.39
comGF/cglF	Streptococcus pneumoniae Rx1	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae D39	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae R6	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae TIGR4	37.857	99.291	0.376