Detailed information    

experimental Experimentally validated

Overview

Name   recG   Type   Machinery gene
Locus tag   KZH42_RS10215 Genome accession   NZ_CP079941
Coordinates   2048188..2050230 (-) Length   680 a.a.
NCBI ID   WP_002225638.1    Uniprot ID   Q9JY17
Organism   Neisseria meningitidis strain C311     
Function   homologous recombination   
Homologous recombination

Function

recG mutant has a substantially reduced transformation frequency.


Genomic Context

Location: 2043188..2055230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH42_RS10195 (KZH42_10190) - 2043813..2044166 (-) 354 WP_002234250.1 hypothetical protein -
  KZH42_RS11690 - 2044159..2044386 (-) 228 WP_002234251.1 hypothetical protein -
  KZH42_RS10200 (KZH42_10195) - 2044395..2045549 (-) 1155 WP_002225636.1 cupin domain-containing protein -
  KZH42_RS12060 (KZH42_10200) - 2045804..2046085 (+) 282 Protein_1991 hypothetical protein -
  KZH42_RS10210 (KZH42_10205) argC 2046082..2047125 (+) 1044 WP_002225637.1 N-acetyl-gamma-glutamyl-phosphate reductase -
  KZH42_RS10215 (KZH42_10210) recG 2048188..2050230 (-) 2043 WP_002225638.1 ATP-dependent DNA helicase RecG Machinery gene
  KZH42_RS10220 (KZH42_10215) secB 2050316..2050759 (-) 444 WP_002225639.1 protein-export chaperone SecB -
  KZH42_RS10225 (KZH42_10220) grxC 2050780..2051037 (-) 258 WP_002221441.1 glutaredoxin 3 -
  KZH42_RS10230 (KZH42_10225) rng 2051250..2052743 (+) 1494 WP_002214463.1 ribonuclease G -
  KZH42_RS10235 (KZH42_10230) - 2052778..2054028 (-) 1251 WP_002225641.1 ATP-binding protein -
  KZH42_RS10240 (KZH42_10235) - 2054024..2054199 (-) 176 Protein_1998 response regulator -
  KZH42_RS10245 (KZH42_10240) - 2054273..2054503 (+) 231 WP_124202315.1 hypothetical protein -

Sequence

Protein


Download         Length: 680 a.a.        Molecular weight: 74859.92 Da        Isoelectric Point: 8.0873

>NTDB_id=1095 KZH42_RS10215 WP_002225638.1 2048188..2050230(-) (recG) [Neisseria meningitidis strain C311]
MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETHIMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQI
ADGSGSVLFLRFIHFYASHQKQTAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKFDELLAQQLSMRLARQKRIGGTA
AALGGDGTLTQALRQALPFALTDAQEKVVSEICRDMAQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEI
LAEQHFIKFKQWLEPLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQHRFGVAQRL
ALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRLVNNVRRAEVEGFVLGTCRKGRQAYWVCPLI
EESETLQLQTAAETLARLQTALPELNIGLVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERM
GLAQLHQLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLGARQSGVPMLRFAKLEE
DLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV

Nucleotide


Download         Length: 2043 bp        

>NTDB_id=1095 KZH42_RS10215 WP_002225638.1 2048188..2050230(-) (recG) [Neisseria meningitidis strain C311]
ATGATGTCGCCCGAAACCCGAAAACAGCTCAAAATCACCGATGTTTCCGCCAAGAAGCTCGACAAACTCAACCTCCATAC
CGCGTGGGATTTGGTGTTGCACCTGCCGCTGCGTTACGAGGACGAGACGCACATTATGCCGATTAAGGACGCGCCGATTG
GCGTGCCGTGTCAGGTCGAGGGGGAGGTTATCCATCAGGAAGTAACGTTCAAACCGCGCAAGCAGCTGATTGTCCAAATC
GCCGACGGCTCCGGCAGCGTCCTTTTTCTGCGCTTCATCCACTTTTACGCCAGCCATCAGAAACAGACGGCGGTCGGCAA
ACGCATCCGCGCCGTGGGCGAAATCAAACACGGCTTTTACGGCGACGAGATGATTCATCCCAAAATCCGCGATGCCGAGG
GCGGCGGTTTGGCGGAAAGCCTCACGCCGGTTTACCCGACCGTAAACGGTTTGAACCAGCCCACTTTGCGCCGTATTATT
CAGACGGCGTTGGACGTTACGCCGCTGCACGACACGCTGCCCGATGCCCTGCTGTGCCGTCTGAAGCTGCCACACCTCGC
CGAAAGCCTGCGTCTTTTGCATTCGCCGCCGCCGAGTTTCACGATTCATCAGCTTTCAGACGGCACGCTGCCTGCATGGC
AACGGCTCAAATTCGATGAACTTTTGGCGCAACAGCTTTCGATGCGCTTGGCGCGACAGAAGCGTATCGGCGGCACGGCG
GCGGCATTGGGCGGCGACGGCACATTGACCCAAGCCCTGCGCCAAGCCCTGCCGTTTGCCCTGACCGATGCGCAAGAAAA
AGTTGTTTCCGAAATCTGCCGCGATATGGCGCAAACCTACCCGATGCACCGCCTGCTGCAGGGCGATGTCGGCAGCGGCA
AAACCATTGTGGCTGCTTTGTCTGCTTTGACGGCTATCGAATCCGGTGCGCAGGTGGCTGTAATGGCGCCCACTGAAATC
CTTGCCGAACAACATTTTATTAAATTTAAACAATGGCTCGAACCTTTGGGCATTGAAGTTGTCCGCCTTTTTGGCAGTTT
GCGTAAAAAAGCCAAAGACGAAGCCAAAGCCAAACTCGCCGACGGCAGCGTCAAAATCGCCGTCGGCACGCACGCCCTGT
TTTCAGACGGCGTGGCGTTTCACAATTTGGGCTTGACCATTGTGGACGAACAGCACCGTTTCGGCGTTGCCCAACGCCTC
GCCCTCAAAAACAAAGGGCGCGAAGTCCATCAGCTGATGATGTCCGCCACGCCCATCCCGCGCACGCTTGCGATGAGTTT
TTTCGCCGATTTGGACGTGTCCGTCATCGACGAATTGCCGCCCGGGCGCACACCGATTAAAACGCGCCTCGTCAACAACG
TCCGCCGCGCCGAAGTCGAAGGCTTCGTCCTCGGCACTTGCCGAAAAGGGCGGCAGGCGTATTGGGTCTGCCCATTGATT
GAAGAAAGCGAAACCCTGCAACTGCAAACCGCCGCCGAAACCCTCGCCCGGCTTCAGACGGCATTGCCCGAACTCAATAT
CGGACTGGTACACGGGCGCATGAAGGCCGCCGAAAAGGCCGAAGTGATGGCGCGGTTTTCTTCGGGGGGGCTGAACGTCT
TGGTCGCCACCACCGTCATCGAAGTCGGCGTAGATGTGCCCAATGCCGCCCTGATGGTCATCGAACACGCCGAGCGCATG
GGCTTGGCGCAGCTGCACCAATTACGCGGTCGGGTCGGGCGCGGCGCGGCAGAAAGCGTGTGCGTCCTCCTGTTTGCCGA
ACCCTTGGGCGAACTCGCCAAAGCGCGGCTGAAAGTCATCTACGAACACACCGACGGCTTTGAAATCGCCCGCCAAGACC
TCAACATCCGCGGCCCCGGCGAATTTCTCGGCGCGCGCCAAAGCGGCGTGCCTATGCTGCGTTTCGCCAAGCTCGAAGAA
GACCTGCACCTCTTGGAACAAGCGCGCGAAACCGCCCCGATGCTGATTGAACAAAACCCCGAAATCGTCGAAGCGCATTT
GGCAAGGTGGCTTTCCGGCAGGGAAGGGTATTTGGGCGTGTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(262-421)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(604-666)

Helicase conserved C-terminal domain (Helicase_C)

(468-575)

RecG wedge domain (RecG_wedge)

(15-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9JY17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG/mmsA Streptococcus pneumoniae R36A

38.872

100

0.39
  recG/mmsA Streptococcus pneumoniae R6

38.872

100

0.39
  recG Bacillus subtilis subsp. subtilis str. 168

39.542

96.324

0.381

References

[1] Yao-Hui Sun et al. (2005) Identification and characterization of genes required for competence in Neisseria meningitidis. Journal of Bacteriology 187(9):3273-6. [PMID: 15838056]