Detailed information    

experimental Experimentally validated

Overview

Name   recG   Type   Machinery gene
Locus tag   KZH42_RS10215 Genome accession   NZ_CP079941
Coordinates   2048188..2050230 (-) Length   680 a.a.
NCBI ID   WP_002225638.1    Uniprot ID   -
Organism   Neisseria meningitidis strain C311     
Function   homologous recombination   
Homologous recombination

Function

recG mutant has a substantially reduced transformation frequency.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2003102..2059748 2048188..2050230 within 0


Gene organization within MGE regions


Location: 2003102..2059748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH42_RS10040 (KZH42_10035) - 2004327..2005277 (+) 951 WP_002219741.1 IS110 family transposase -
  KZH42_RS10045 (KZH42_10040) - 2005437..2005592 (+) 156 Protein_1958 hemagglutinin repeat-containing protein -
  KZH42_RS10050 (KZH42_10045) - 2005663..2005920 (-) 258 Protein_1959 transposase -
  KZH42_RS11670 - 2006240..2006353 (-) 114 WP_002219744.1 helix-turn-helix domain-containing protein -
  KZH42_RS10060 (KZH42_10055) - 2007426..2007863 (+) 438 WP_002219746.1 virulence-associated protein VapD -
  KZH42_RS11675 - 2009398..2009595 (-) 198 WP_225905368.1 zinc ribbon domain-containing protein -
  KZH42_RS10070 (KZH42_10065) - 2010181..2011545 (+) 1365 WP_002222955.1 Fic family protein -
  KZH42_RS10075 (KZH42_10070) - 2012053..2012412 (+) 360 WP_002227070.1 hypothetical protein -
  KZH42_RS10080 (KZH42_10075) - 2012539..2013828 (+) 1290 WP_002225622.1 type II toxin-antitoxin system HipA family toxin -
  KZH42_RS10085 (KZH42_10080) - 2014153..2015940 (-) 1788 WP_002225623.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -
  KZH42_RS10090 (KZH42_10085) - 2015995..2016453 (-) 459 WP_002224037.1 protein-lysine palmitoyltransferase -
  KZH42_RS10095 (KZH42_10090) - 2016619..2016933 (-) 315 WP_002225624.1 hypothetical protein -
  KZH42_RS10100 (KZH42_10095) - 2016945..2017253 (-) 309 WP_002233812.1 hypothetical protein -
  KZH42_RS10105 (KZH42_10100) - 2017225..2018226 (-) 1002 WP_225905348.1 hemagglutinin -
  KZH42_RS10110 (KZH42_10105) - 2018307..2018723 (-) 417 WP_002224040.1 hypothetical protein -
  KZH42_RS12055 - 2018727..2019329 (-) 603 WP_372238191.1 DUF6973 domain-containing protein -
  KZH42_RS10115 (KZH42_10110) - 2019324..2026271 (-) 6948 Protein_1973 hemagglutinin repeat-containing protein -
  KZH42_RS10120 (KZH42_10115) - 2026427..2026585 (+) 159 Protein_1974 transposase -
  KZH42_RS10125 (KZH42_10120) - 2026850..2027815 (+) 966 WP_002221064.1 IS30-like element IS1655 family transposase -
  KZH42_RS10130 (KZH42_10125) - 2027883..2028317 (-) 435 WP_010980984.1 hypothetical protein -
  KZH42_RS11680 - 2028314..2030101 (-) 1788 WP_225905349.1 DUF637 domain-containing protein -
  KZH42_RS10145 (KZH42_10140) - 2030187..2030561 (-) 375 WP_002224016.1 hypothetical protein -
  KZH42_RS10150 (KZH42_10145) - 2030839..2031216 (-) 378 WP_002217880.1 hypothetical protein -
  KZH42_RS10155 (KZH42_10150) - 2031225..2032904 (-) 1680 WP_050542144.1 S-layer family protein -
  KZH42_RS10160 (KZH42_10155) - 2032973..2033482 (-) 510 WP_002219760.1 hypothetical protein -
  KZH42_RS10165 (KZH42_10160) hrpA 2033489..2039425 (-) 5937 WP_002225630.1 two-partner secretion exoprotein HrpA -
  KZH42_RS10170 (KZH42_10165) hrpB 2039534..2041276 (-) 1743 WP_002225635.1 two-partner secretion system transporter HrpB -
  KZH42_RS11685 - 2041424..2041576 (-) 153 Protein_1984 cysteine peptidase family C39 domain-containing protein -
  KZH42_RS10180 (KZH42_10175) - 2041860..2042162 (-) 303 WP_002221436.1 hypothetical protein -
  KZH42_RS10185 (KZH42_10180) - 2042528..2042971 (-) 444 WP_002232972.1 HlyD family efflux transporter periplasmic adaptor subunit -
  KZH42_RS10190 (KZH42_10185) - 2043036..2043617 (-) 582 WP_225905350.1 HlyD family secretion protein -
  KZH42_RS10195 (KZH42_10190) - 2043813..2044166 (-) 354 WP_002234250.1 hypothetical protein -
  KZH42_RS11690 - 2044159..2044386 (-) 228 WP_002234251.1 hypothetical protein -
  KZH42_RS10200 (KZH42_10195) - 2044395..2045549 (-) 1155 WP_002225636.1 cupin domain-containing protein -
  KZH42_RS12060 (KZH42_10200) - 2045804..2046085 (+) 282 Protein_1991 hypothetical protein -
  KZH42_RS10210 (KZH42_10205) argC 2046082..2047125 (+) 1044 WP_002225637.1 N-acetyl-gamma-glutamyl-phosphate reductase -
  KZH42_RS10215 (KZH42_10210) recG 2048188..2050230 (-) 2043 WP_002225638.1 ATP-dependent DNA helicase RecG Machinery gene
  KZH42_RS10220 (KZH42_10215) secB 2050316..2050759 (-) 444 WP_002225639.1 protein-export chaperone SecB -
  KZH42_RS10225 (KZH42_10220) grxC 2050780..2051037 (-) 258 WP_002221441.1 glutaredoxin 3 -
  KZH42_RS10230 (KZH42_10225) rng 2051250..2052743 (+) 1494 WP_002214463.1 ribonuclease G -
  KZH42_RS10235 (KZH42_10230) - 2052778..2054028 (-) 1251 WP_002225641.1 ATP-binding protein -
  KZH42_RS10240 (KZH42_10235) - 2054024..2054199 (-) 176 Protein_1998 response regulator -
  KZH42_RS10245 (KZH42_10240) - 2054273..2054503 (+) 231 WP_124202315.1 hypothetical protein -
  KZH42_RS10250 (KZH42_10245) - 2054422..2055735 (-) 1314 WP_002222940.1 CitMHS family transporter -
  KZH42_RS10255 (KZH42_10250) - 2056175..2056315 (-) 141 WP_002214468.1 hypothetical protein -
  KZH42_RS10260 (KZH42_10255) - 2056305..2056871 (-) 567 WP_002222966.1 NADPH-dependent FMN reductase -
  KZH42_RS10265 (KZH42_10260) dacB 2057036..2058445 (+) 1410 WP_002225643.1 D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase -
  KZH42_RS10270 (KZH42_10265) - 2058492..2059163 (-) 672 Protein_2004 IS1595 family transposase -

Sequence

Protein


Download         Length: 680 a.a.        Molecular weight: 74859.92 Da        Isoelectric Point: 8.0873

>NTDB_id=1095 KZH42_RS10215 WP_002225638.1 2048188..2050230(-) (recG) [Neisseria meningitidis strain C311]
MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETHIMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQI
ADGSGSVLFLRFIHFYASHQKQTAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKFDELLAQQLSMRLARQKRIGGTA
AALGGDGTLTQALRQALPFALTDAQEKVVSEICRDMAQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEI
LAEQHFIKFKQWLEPLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQHRFGVAQRL
ALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRLVNNVRRAEVEGFVLGTCRKGRQAYWVCPLI
EESETLQLQTAAETLARLQTALPELNIGLVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERM
GLAQLHQLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLGARQSGVPMLRFAKLEE
DLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV

Nucleotide


Download         Length: 2043 bp        

>NTDB_id=1095 KZH42_RS10215 WP_002225638.1 2048188..2050230(-) (recG) [Neisseria meningitidis strain C311]
ATGATGTCGCCCGAAACCCGAAAACAGCTCAAAATCACCGATGTTTCCGCCAAGAAGCTCGACAAACTCAACCTCCATAC
CGCGTGGGATTTGGTGTTGCACCTGCCGCTGCGTTACGAGGACGAGACGCACATTATGCCGATTAAGGACGCGCCGATTG
GCGTGCCGTGTCAGGTCGAGGGGGAGGTTATCCATCAGGAAGTAACGTTCAAACCGCGCAAGCAGCTGATTGTCCAAATC
GCCGACGGCTCCGGCAGCGTCCTTTTTCTGCGCTTCATCCACTTTTACGCCAGCCATCAGAAACAGACGGCGGTCGGCAA
ACGCATCCGCGCCGTGGGCGAAATCAAACACGGCTTTTACGGCGACGAGATGATTCATCCCAAAATCCGCGATGCCGAGG
GCGGCGGTTTGGCGGAAAGCCTCACGCCGGTTTACCCGACCGTAAACGGTTTGAACCAGCCCACTTTGCGCCGTATTATT
CAGACGGCGTTGGACGTTACGCCGCTGCACGACACGCTGCCCGATGCCCTGCTGTGCCGTCTGAAGCTGCCACACCTCGC
CGAAAGCCTGCGTCTTTTGCATTCGCCGCCGCCGAGTTTCACGATTCATCAGCTTTCAGACGGCACGCTGCCTGCATGGC
AACGGCTCAAATTCGATGAACTTTTGGCGCAACAGCTTTCGATGCGCTTGGCGCGACAGAAGCGTATCGGCGGCACGGCG
GCGGCATTGGGCGGCGACGGCACATTGACCCAAGCCCTGCGCCAAGCCCTGCCGTTTGCCCTGACCGATGCGCAAGAAAA
AGTTGTTTCCGAAATCTGCCGCGATATGGCGCAAACCTACCCGATGCACCGCCTGCTGCAGGGCGATGTCGGCAGCGGCA
AAACCATTGTGGCTGCTTTGTCTGCTTTGACGGCTATCGAATCCGGTGCGCAGGTGGCTGTAATGGCGCCCACTGAAATC
CTTGCCGAACAACATTTTATTAAATTTAAACAATGGCTCGAACCTTTGGGCATTGAAGTTGTCCGCCTTTTTGGCAGTTT
GCGTAAAAAAGCCAAAGACGAAGCCAAAGCCAAACTCGCCGACGGCAGCGTCAAAATCGCCGTCGGCACGCACGCCCTGT
TTTCAGACGGCGTGGCGTTTCACAATTTGGGCTTGACCATTGTGGACGAACAGCACCGTTTCGGCGTTGCCCAACGCCTC
GCCCTCAAAAACAAAGGGCGCGAAGTCCATCAGCTGATGATGTCCGCCACGCCCATCCCGCGCACGCTTGCGATGAGTTT
TTTCGCCGATTTGGACGTGTCCGTCATCGACGAATTGCCGCCCGGGCGCACACCGATTAAAACGCGCCTCGTCAACAACG
TCCGCCGCGCCGAAGTCGAAGGCTTCGTCCTCGGCACTTGCCGAAAAGGGCGGCAGGCGTATTGGGTCTGCCCATTGATT
GAAGAAAGCGAAACCCTGCAACTGCAAACCGCCGCCGAAACCCTCGCCCGGCTTCAGACGGCATTGCCCGAACTCAATAT
CGGACTGGTACACGGGCGCATGAAGGCCGCCGAAAAGGCCGAAGTGATGGCGCGGTTTTCTTCGGGGGGGCTGAACGTCT
TGGTCGCCACCACCGTCATCGAAGTCGGCGTAGATGTGCCCAATGCCGCCCTGATGGTCATCGAACACGCCGAGCGCATG
GGCTTGGCGCAGCTGCACCAATTACGCGGTCGGGTCGGGCGCGGCGCGGCAGAAAGCGTGTGCGTCCTCCTGTTTGCCGA
ACCCTTGGGCGAACTCGCCAAAGCGCGGCTGAAAGTCATCTACGAACACACCGACGGCTTTGAAATCGCCCGCCAAGACC
TCAACATCCGCGGCCCCGGCGAATTTCTCGGCGCGCGCCAAAGCGGCGTGCCTATGCTGCGTTTCGCCAAGCTCGAAGAA
GACCTGCACCTCTTGGAACAAGCGCGCGAAACCGCCCCGATGCTGATTGAACAAAACCCCGAAATCGTCGAAGCGCATTT
GGCAAGGTGGCTTTCCGGCAGGGAAGGGTATTTGGGCGTGTGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(262-421)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(604-666)

Helicase conserved C-terminal domain (Helicase_C)

(468-575)

RecG wedge domain (RecG_wedge)

(15-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yao-Hui Sun et al. (2005) Identification and characterization of genes required for competence in Neisseria meningitidis. Journal of Bacteriology 187(9):3273-6. [PMID: 15838056]