Detailed information NTG0000657

Experimentally validated

Overview

• Name: recG/mmsA
• Type: Machinery gene
• Locus tag: SPR_RS07635
• Genome accession: NC_003098
• Coordinates: 1520712..1522727 (-)
• Length: 671 a.a.
• NCBI ID: WP_001048745.1
• Uniprot ID: Q7ZAK6
• Organism: Streptococcus pneumoniae R6
• Function: homologous recombination Homologous recombination

Function

MmsA recognizes and branch migrates three-strand transformation intermediates to extend donor-recipient heteroduplex regions. The mmsA-null mutant exhibited the other phenotypes of the original mutant, apart from mismatch-repair deficiency and, in addition, an alteration in colony-forming ability was noticed.

Genomic Context

Location: 1515712..1527727

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS07620 (spr1537)	-	1518294..1518521 (-)	228	WP_000576349.1	hypothetical protein	-
SPR_RS07625	-	1518922..1519062 (+)	141	WP_001808987.1	hypothetical protein	-
SPR_RS07630 (spr1538)	-	1519416..1520396 (-)	981	WP_000795124.1	acetylxylan esterase	-
SPR_RS07635 (spr1539)	recG/mmsA	1520712..1522727 (-)	2016	WP_001048745.1	ATP-dependent DNA helicase RecG	Machinery gene
SPR_RS07640 (spr1540)	alr	1522746..1523849 (-)	1104	WP_000648075.1	alanine racemase	-
SPR_RS07645 (spr1541)	acpS	1523839..1524201 (-)	363	WP_000635008.1	holo-ACP synthase	-
SPR_RS07650 (spr1542)	-	1524246..1525277 (-)	1032	WP_000864060.1	3-deoxy-7-phosphoheptulonate synthase	-
SPR_RS07655 (spr1543)	-	1525279..1526310 (-)	1032	WP_000232212.1	3-deoxy-7-phosphoheptulonate synthase	-

Sequence

Protein

• Length: 671 a.a.
• Molecular weight: 75188.76 Da
• Isoelectric Point: 6.1845

>NTDB_id=657 SPR_RS07635 WP_001048745.1 1520712..1522727(-) (recG/mmsA) [Streptococcus pneumoniae R6]
MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFKTKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLR
FSLKQGEVVFAVNFFNQPYLADKIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFAELFYFQMQLQTLKSENRVQGSGLVLNWSQE
KVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNRLLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFES
LQNLFPNLKLALLTGSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRILREKGDNPD
VLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVLTWLEGEIQKGSQAYVISPLIEESEALDLKN
AIALSEELTTHFAGKAEVALLHGRMKSDEKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLR
GRVGRGDKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQVADIIEDFPILEEARK
VASYISSIEAWQEDPEWRMIALHLEKKEHLD

Nucleotide

• Length: 2016 bp

>NTDB_id=657 SPR_RS07635 WP_001048745.1 1520712..1522727(-) (recG/mmsA) [Streptococcus pneumoniae R6]
ATGAATCTACATCAACCCTTGCATGTCTTGCCTGGTGTGGGACCAAAGTCAGCAGAAAAATACGCCAAACTAGGAATTGA
AAACTTGCAAGATCTCTTGCTCTACTTTCCTTTCCGTTATGAAGACTTCAAAACCAAGCAGGTGCTGGAGCTGGAAGACG
GTGAGAAGGCAGTTCTTTCTGGTCAGGTAGTGACTCCTGCTAGTGTCCAGTATTATGGTTTCAAGCGCAATCGCCTGCGT
TTTAGTCTCAAGCAGGGAGAGGTCGTTTTTGCGGTGAATTTCTTTAACCAGCCCTATCTGGCTGATAAAATAGAGTTGGG
AGCAACCCTTGCTGTCTTTGGAAAATGGGACCGCGCTAAGGCTAGTCTGACTGGGATGAAGGTTCTGGCTCAGGTAGAAG
ATGACCTCCAGCCTGTCTATCGTCTGGCTCAGGGAATCAGTCAGGCCAGTCTGGTTAAGGTCATCAAGACGGCTTTTGAT
CAGGGACTGGACCTCTTGATAGAAGAAAATCTGCCCCAGTCTTTACTAGACAAATACAAACTCATGTCCCGTTGTCAGGC
AGTCCGAGCTATGCATTTTCCAAAGGATTTGGCAGAATACAAGCAGGCTCTTCGCCGTATCAAGTTTGCGGAACTCTTTT
ATTTCCAAATGCAGTTGCAGACGCTCAAGTCTGAAAATAGAGTTCAGGGAAGTGGTCTGGTTCTGAATTGGTCTCAGGAA
AAAGTGACAGCAGTTAAAGCAAGTCTTCCTTTTGCCCTGACCCAAGCTCAGGAAAAGAGTTTGCAGGAAATTTTAACTGA
TATGAAGTCCGACCACCACATGAATCGTCTCCTACAAGGGGATGTGGGGAGTGGAAAAACGGTAGTCGCTGGCTTGGCCA
TGTTTGCGGCAGTGACAGCAGGTTATCAGGCTGCCCTAATGGTACCAACAGAAATCCTCGCAGAGCAACACTTTGAGAGT
TTACAGAACCTTTTTCCCAATTTGAAACTGGCTCTCTTGACAGGTTCCTTGAAAGCTGCAGAAAAGAGAGAAGTCTTGGA
GACCATTGCCAAGGGTGAGGCTGATTTGATTATAGGAACTCACGCTCTGATACAAGATGGGGTGGAGTATGCTCGTCTTG
GTTTGATTATTATCGATGAGCAGCACCGTTTTGGTGTAGGGCAAAGGCGTATTTTACGGGAAAAAGGTGACAATCCAGAT
GTCCTCATGATGACGGCGACTCCCATTCCACGGACGCTTGCCATCACAGCCTTTGGAGATATGGATGTTTCCATTATCGA
CCAGATGCCAGCAGGTCGGAAGCCTATTGTGACGCGCTGGATCAAACATGAGCAACTACCTCAGGTCTTGACTTGGTTAG
AGGGGGAAATTCAAAAAGGTTCCCAAGCCTATGTCATCTCTCCTTTGATTGAAGAATCAGAAGCTCTAGATTTGAAAAAT
GCCATTGCCTTATCAGAGGAGTTGACGACTCATTTTGCAGGCAAGGCAGAGGTGGCTCTTCTACATGGTAGGATGAAGAG
TGACGAAAAAGACCAGATCATGCAGGATTTCAAGGAGAGAAAGACGGATATTCTGGTTTCGACGACGGTTATTGAGGTTG
GGGTCAACGTTCCCAATGCGACTGTCATGATTATCATGGATGCCGATCGCTTCGGTCTCAGTCAACTTCACCAGCTTAGA
GGTCGTGTCGGTCGGGGGGACAAGCAGTCCTACGCTGTTCTCGTTGCCAATCCCAAGACGGATTCTGGGAAAGACCGCAT
GCGCATCATGACAGAAACGACCAATGGATTTGTCCTTGCGGAGGAAGATTTGAAAATGCGTGGTTCTGGTGAGATTTTTG
GAACCAGACAGTCAGGACTTCCAGAGTTCCAAGTGGCTGATATTATCGAAGATTTTCCGATTTTAGAAGAAGCAAGAAAG
GTTGCTAGCTACATTAGTTCTATAGAAGCTTGGCAAGAAGATCCAGAGTGGCGCATGATTGCCCTTCATCTGGAAAAGAA
AGAACATCTGGATTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (456-566)

• DEAD/DEAH box helicase (DEAD) (254-411)

• RecG wedge domain (RecG_wedge) (11-158)

• ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C) (593-665)

3D structure

• AlphaFold DB: Q7ZAK6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recG/mmsA	Streptococcus pneumoniae R36A	100	100	1
recG	Bacillus subtilis subsp. subtilis str. 168	48.968	100	0.495
recG	Neisseria meningitidis strain C311	38.872	100	0.39

References

• [1]: Mohammad A Hedayati et al. (2002) Effect of the Streptococcus pneumoniae MmsA protein on the RecA protein-promoted three-strand exchange reaction. Implications for the mechanism of transformational recombination. The Journal of Biological Chemistry 277(28):24863-9. [PMID: 11960988]
• [2]: I Mortier-Barriere et al. (1997) Control of recombination rate during transformation of Streptococcus pneumoniae: an overview. Microbial Drug Resistance (Larchmont, N.Y.) 3(3):233-42. [PMID: 9270992]
• [3]: B Martin et al. (1996) The mmsA locus of Streptococcus pneumoniae encodes a RecG-like protein involved in DNA repair and in three-strand recombination. Molecular Microbiology 19(5):1035-45. [PMID: 8830261]