Detailed information NTG0000630

Experimentally validated

Overview

• Name: recG
• Type: Machinery gene
• Locus tag: BSU_15870
• Genome accession: NC_000964
• Coordinates: 1659810..1661858 (+)
• Length: 682 a.a.
• NCBI ID: NP_389469.1
• Uniprot ID: O34942
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: homologous recombination Homologous recombination

Function

Intramolecular homologous recombination was dependent on RecF, RecB, RecG, RecP, RecH and AddAB functions.

Genomic Context

Location: 1654810..1666858

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_15810 (BSU15810)	spoVM	1655446..1655526 (+)	81	NP_389463.1	factor required for normal spore cortex and coat synthesis (stage V sporulation)	-
BSU_15820 (BSU15820)	rpmB	1655599..1655787 (-)	189	NP_389464.1	ribosomal protein L28	-
BSU_15830 (BSU15830)	yloU	1656064..1656426 (+)	363	NP_389465.1	putative factor involved in malonyl-CoA synthesis	-
BSU_15840 (BSU15840)	yloV	1656442..1658103 (+)	1662	NP_389466.1	putative enzyme structurally related to dihydroxyacetone/glyceraldehyde kinase	-
BSU_15850 (BSU15850)	sdaAB	1658242..1658904 (+)	663	NP_389467.1	L-serine dehydratase (beta chain)	-
BSU_15860 (BSU15860)	sdaAA	1658930..1659832 (+)	903	NP_389468.1	L-serine dehydratase (alpha chain)	-
BSU_15870 (BSU15870)	recG	1659810..1661858 (+)	2049	NP_389469.1	branch migrating ATP-dependent DNA helicase involved in DNA recombination and repair	Machinery gene
BSU_15880 (BSU15880)	fapR	1661967..1662533 (+)	567	NP_389470.1	transcription factor controlling fatty acid and phospholipid metabolism (FapR-malonyl-Coa)	-
BSU_15890 (BSU15890)	plsX	1662547..1663548 (+)	1002	NP_389471.1	phosphate:acyl-ACP acyltransferase	-
BSU_15900 (BSU15900)	fabD	1663567..1664520 (+)	954	NP_389472.1	malonyl CoA:acyl carrier protein transacylase	-
BSU_15910 (BSU15910)	fabG	1664513..1665253 (+)	741	NP_389473.1	beta-ketoacyl-acyl carrier protein reductase	-
BSU_15920 (BSU15920)	acpA	1665337..1665570 (+)	234	NP_389474.1	acyl carrier protein	-
BSU_15930 (BSU15930)	rnc	1665710..1666459 (+)	750	NP_389475.1	ribonuclease III	-

Sequence

Protein

• Length: 682 a.a.
• Molecular weight: 78140.72 Da
• Isoelectric Point: 7.4260

>NTDB_id=630 BSU_15870 NP_389469.1 1659810..1661858(+) (recG) [Bacillus subtilis subsp. subtilis str. 168]
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide

• Length: 2049 bp

>NTDB_id=630 BSU_15870 NP_389469.1 1659810..1661858(+) (recG) [Bacillus subtilis subsp. subtilis str. 168]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGAAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCATGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGTTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTATTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGATCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTAAATGTTCCGAATGCAACAATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (260-415)

• Helicase conserved C-terminal domain (Helicase_C) (452-572)

• ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C) (599-670)

• RecG wedge domain (RecG_wedge) (9-165)

3D structure

• AlphaFold DB: O34942

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recG/mmsA	Streptococcus pneumoniae R36A	48.968	100	0.495
recG/mmsA	Streptococcus pneumoniae R6	48.968	100	0.495
recG	Neisseria meningitidis strain C311	39.542	96.324	0.381

References

• [1]: Rubén Torres et al. (2021) DisA Limits RecG Activities at Stalled or Reversed Replication Forks. Cells 10(6):1357. [PMID: 34073022]
• [2]: Rubén Torres et al. (2017) Interplay between Bacillus subtilis RecD2 and the RecG or RuvAB helicase in recombinational repair. DNA Repair 55:40-46. [PMID: 28527403]
• [3]: Humberto Sanchez et al. (2007) Bacillus subtilis RecG branch migration translocase is required for DNA repair and chromosomal segregation. Molecular Microbiology 65(4):920-35. [PMID: 17640277]
• [4]: Begoña Carrasco et al. (2004) Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation. Journal of Bacteriology 186(17):5557-66. [PMID: 15317759]
• [5]: J C Alonso et al. (1992) Intramolecular homologous recombination in Bacillus subtilis 168. Molecular & General Genetics : MGG 236(1):60-4. [PMID: 1494351]