Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLJM2_RS11220 Genome accession   NZ_CP015900
Coordinates   2168540..2169565 (-) Length   341 a.a.
NCBI ID   WP_050574185.1    Uniprot ID   -
Organism   Lactococcus cremoris strain JM2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2162146..2170376 2168540..2169565 within 0


Gene organization within MGE regions


Location: 2162146..2170376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM2_RS11175 (LLJM2_2193) - 2163681..2164490 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLJM2_RS11180 (LLJM2_2194) - 2164483..2165220 (-) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLJM2_RS11185 (LLJM2_2195) - 2165399..2166241 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLJM2_RS11190 (LLJM2_2196) - 2166238..2166675 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLJM2_RS11195 (LLJM2_03575) comGG 2166755..2166982 (-) 228 WP_228764408.1 competence protein ComGG Machinery gene
  LLJM2_RS11200 (LLJM2_2198) comGF 2167078..2167503 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLJM2_RS11205 (LLJM2_2199) comGE 2167487..2167723 (-) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLJM2_RS11210 (LLJM2_2200) comGD 2167755..2167943 (-) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLJM2_RS11215 (LLJM2_2201) comGC 2168145..2168522 (-) 378 Protein_2171 competence type IV pilus major pilin ComGC -
  LLJM2_RS11220 (LLJM2_2202) comGB 2168540..2169565 (-) 1026 WP_050574185.1 competence type IV pilus assembly protein ComGB Machinery gene

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38393.18 Da        Isoelectric Point: 9.9840

>NTDB_id=182999 LLJM2_RS11220 WP_050574185.1 2168540..2169565(-) (comGB) [Lactococcus cremoris strain JM2]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILDSLSCSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLLVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKVPLVSSFIRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKIRIF
AEVGIKLDLGLKAGQNFEQAVSHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMSLPIYSNMGSGI

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=182999 LLJM2_RS11220 WP_050574185.1 2168540..2169565(-) (comGB) [Lactococcus cremoris strain JM2]
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGATAGTCTCTCTTGTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTGGTATTTCTCATTGGAATTA
TGTGGGGCTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTGACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTTAAAGTTCCATTAGTAAGTTCTTTTATCCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGAGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAATTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGCTGGTCAGAATTTTGAGCAAGCTGTCAGTCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCGATGTCGTTACCCATTTATTCTAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(211-332)

Type II secretion system (T2SS), protein F (T2SSF)

(25-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

97.654

100

0.977
  comYB Streptococcus gordonii str. Challis substr. CH1

51.039

98.827

0.504
  comGB/cglB Streptococcus pneumoniae D39

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae R6

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae Rx1

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae TIGR4

50.299

97.947

0.493
  comGB/cglB Streptococcus mitis SK321

50

97.947

0.49
  comGB/cglB Streptococcus mitis NCTC 12261

49.102

97.947

0.481
  comYB Streptococcus mutans UA140

50.955

92.082

0.469
  comYB Streptococcus mutans UA159

50.318

92.082

0.463

Multiple sequence alignment