Detailed information NTG0182984

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLJM2_RS09270
• Genome accession: NZ_CP015900
• Coordinates: 1808571..1809017 (-)
• Length: 148 a.a.
• NCBI ID: WP_228763864.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain JM2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1803571..1814017

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJM2_RS09240 (LLJM2_1819)	-	1803915..1804130 (-)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-
LLJM2_RS09245 (LLJM2_1820)	-	1804313..1805089 (-)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
LLJM2_RS09250 (LLJM2_1821)	-	1805373..1805678 (-)	306	Protein_1795	ComEC/Rec2 family competence protein	-
LLJM2_RS09255 (LLJM2_03370)	-	1805699..1806589 (-)	891	WP_281634793.1	IS982-like element ISLll1 family transposase	-
LLJM2_RS09260 (LLJM2_03375)	comEC	1806666..1807175 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLJM2_RS09265 (LLJM2_1824)	comEC	1807172..1808443 (-)	1272	WP_324187043.1	ComEC/Rec2 family competence protein	Machinery gene
LLJM2_RS09270 (LLJM2_1825)	comEA	1808571..1809017 (-)	447	WP_228763864.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLJM2_RS09275 (LLJM2_1826)	-	1809283..1810644 (-)	1362	WP_081196831.1	ABC transporter permease	-
LLJM2_RS09280 (LLJM2_1827)	-	1810641..1811573 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
LLJM2_RS09285 (LLJM2_1828)	-	1811679..1812077 (-)	399	WP_011676775.1	hypothetical protein	-
LLJM2_RS09290 (LLJM2_1829)	-	1812169..1812753 (-)	585	WP_081196832.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 16038.01 Da
• Isoelectric Point: 4.6909

>NTDB_id=182984 LLJM2_RS09270 WP_228763864.1 1808571..1809017(-) (comEA) [Lactococcus cremoris strain JM2]
MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVS
TTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 447 bp

>NTDB_id=182984 LLJM2_RS09270 WP_228763864.1 1808571..1809017(-) (comEA) [Lactococcus cremoris strain JM2]
ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG
GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTT
ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCA
ACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA
AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG
GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (1-56)

• Helix-hairpin-helix motif (HHH_3) (84-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	96.622	100	0.966
comEA/celA/cilE	Streptococcus mitis SK321	51.02	99.324	0.507
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.493
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.98	99.324	0.486
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae D39	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae R6	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.619	99.324	0.473
comEA	Streptococcus thermophilus LMD-9	43.421	100	0.446
comEA	Staphylococcus aureus N315	41.781	98.649	0.412
comEA	Staphylococcus aureus MW2	41.781	98.649	0.412
comEA	Bacillus subtilis subsp. subtilis str. 168	40.816	99.324	0.405