Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LL158_RS10245 Genome accession   NZ_CP015894
Coordinates   2029031..2030392 (-) Length   453 a.a.
NCBI ID   WP_011677138.1    Uniprot ID   A0A165G707
Organism   Lactococcus cremoris strain 158     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2024031..2035392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL158_RS10210 (LL158_1972) - 2024132..2024455 (-) 324 WP_011677132.1 DUF1048 domain-containing protein -
  LL158_RS10215 (LL158_1973) - 2024452..2024790 (-) 339 WP_011677133.1 DUF1048 domain-containing protein -
  LL158_RS10220 (LL158_1974) - 2024783..2025139 (-) 357 WP_011677134.1 PadR family transcriptional regulator -
  LL158_RS10225 (LL158_1975) - 2025261..2025890 (-) 630 WP_011677135.1 hypothetical protein -
  LL158_RS10230 (LL158_1976) - 2025948..2027033 (-) 1086 WP_015082908.1 PIN/TRAM domain-containing protein -
  LL158_RS10235 (LL158_1977) - 2027300..2027983 (+) 684 WP_015082909.1 YoaK family protein -
  LL158_RS10240 (LL158_1978) - 2028040..2028930 (+) 891 WP_015082910.1 IS982 family transposase -
  LL158_RS10245 (LL158_1979) radA 2029031..2030392 (-) 1362 WP_011677138.1 DNA repair protein RadA Machinery gene
  LL158_RS10250 (LL158_1980) - 2030562..2031575 (-) 1014 WP_021164998.1 glycosyltransferase family 2 protein -
  LL158_RS10255 (LL158_1981) - 2031557..2032102 (-) 546 WP_015082912.1 HAD-IIIA family hydrolase -
  LL158_RS10260 (LL158_1982) - 2032099..2032674 (-) 576 WP_015082913.1 SIS domain-containing protein -
  LL158_RS10265 (LL158_1983) - 2032736..2033530 (-) 795 WP_014573315.1 GHMP kinase -
  LL158_RS10270 (LL158_1984) - 2033739..2034746 (-) 1008 WP_228763944.1 lipopolysaccharide biosynthesis protein -
  LL158_RS10275 (LL158_03210) - 2034800..2035261 (-) 462 WP_228763943.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49897.33 Da        Isoelectric Point: 5.2965

>NTDB_id=182845 LL158_RS10245 WP_011677138.1 2029031..2030392(-) (radA) [Lactococcus cremoris strain 158]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMSGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIKVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=182845 LL158_RS10245 WP_011677138.1 2029031..2030392(-) (radA) [Lactococcus cremoris strain 158]
ATGGCGAAGAAAAAATCATCTTTTGTTTGTCAAAATTGTGGTTATAAATCTGCAAAGTATCTTGGGCGATGCCCAAATTG
TGGAGAGTGGTCCTCTTTTGTTGAAGAAGTTGAAGTACAGGAAGTCAAAAATCAGCGAGTGTCGATGTCTGGTGAACGTT
CAAAACCAATGAAATTAGATGAAGTTGAGCTTTTTGATACACCACGTATTGAAACAGATCTTGACGAATTTAATCGTGTT
TTAGGTGGTGGTGTTGTTCCTGGTTCACTCGTTTTGATTGGTGGAGACCCAGGAATAGGAAAATCTACGTTGCTTTTGCA
AGTATCGACTCAGCTTGCATCAAGAGGACGGGTTCTCTATGTGAGTGGTGAAGAATCTGCTCAACAAATTAAGCTTAGAG
CGGAACGTTTGGGCGATATTGATCGAGATTTTTATCTTTACGCCGAGACTAACATGCAGTCAATTCGTGCTGAAGTTGAA
CGTTTGAAGCCAAATTTTTTGATTATTGATTCGATCCAAACTATTATGACACCTGAAATTCAATCAACTCAAGGATCTGT
GAGTCAAGTTCGAGAAGTGACAGGTGAACTCATGCAAATTGCCAAAACGAATGATATTGCTACTTTTATCGTTGGTCATG
TGACGAAAGAAGGTCAATTGGCAGGACCAAGAATGCTAGAGCACATGGTTGATACCGTTCTTTATTTTGAAGGAGAAAGA
AATAATACTTTTAGAATTCTTCGAGCAGTTAAAAACCGGTTTGGGTCAACCAATGAAATTGGTATATTCGAAATGCAAGG
GCATGGTTTAGTTGAAGTAACTAATCCTTCTGAGGTTTTCTTGGAAGAGCGATTAGAGGGTTCAACTGGTTCTGCTATTG
TTTGTGCACTCGAAGGGACACGTCCGATATTAGTTGAAATACAGGCTTTAACTACACCAACAATGTTTGGAAATGCTAAG
AGAACAACTTCTGGTTTAGATTTTAATAGAGTGAGCCTGATTATGGCAGTACTTGAAAAACGGACAGGCTTATTGATGCA
AAATCAAGATGCTTACCTTAAATCAGCTGGGGGAGTAAAATTAGATGAGCCAGCCATTGACCTTGCGGTTGCTGTCGCTG
TAGCCTCAAGTTATAAAGAACTTCCGACGGATGCGCGTGAATGTTTTATTGGTGAAATTGGCTTAACAGGAGAGATTCGT
CGTGTGACGCGAATTGAACAAAGACTGAATGAAGCTGCTAAATTAGGATTCAAAAAAGTCTATGCTCCTAAAAATTCAAT
TGTGGGAATTGATATTCCTGAACAAATAAAAGTAATTGGGGTAACAACCCTAACTGAATGTCTAAAATTAGTATTTGGCT
AA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.707

100

0.623

Multiple sequence alignment