Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LL158_RS10555 Genome accession   NZ_CP015894
Coordinates   2074045..2074470 (-) Length   141 a.a.
NCBI ID   WP_373467301.1    Uniprot ID   -
Organism   Lactococcus cremoris strain 158     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2069135..2073589 2074045..2074470 flank 456


Gene organization within MGE regions


Location: 2069135..2074470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL158_RS10530 (LL158_03375) - 2070648..2071457 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LL158_RS10535 (LL158_03380) - 2071450..2072187 (-) 738 WP_015082929.1 metal ABC transporter ATP-binding protein -
  LL158_RS10540 (LL158_03385) - 2072366..2073208 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LL158_RS10545 (LL158_03390) - 2073205..2073642 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LL158_RS10550 (LL158_03395) comGG 2073722..2074021 (-) 300 WP_011677181.1 competence type IV pilus minor pilin ComGG Machinery gene
  LL158_RS10555 (LL158_03400) comGF 2074045..2074470 (-) 426 WP_373467301.1 competence type IV pilus minor pilin ComGF Machinery gene

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 16268.81 Da        Isoelectric Point: 7.7759

>NTDB_id=182849 LL158_RS10555 WP_373467301.1 2074045..2074470(-) (comGF) [Lactococcus cremoris strain 158]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGEKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=182849 LL158_RS10555 WP_373467301.1 2074045..2074470(-) (comGF) [Lactococcus cremoris strain 158]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGAAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

99.291

100

0.993
  comYF Streptococcus mutans UA140

43.972

100

0.44
  comYF Streptococcus mutans UA159

43.262

100

0.433
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

99.291

0.39
  comGF/cglF Streptococcus mitis SK321

39.286

99.291

0.39
  comGF/cglF Streptococcus pneumoniae Rx1

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae D39

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae R6

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae TIGR4

37.857

99.291

0.376

Multiple sequence alignment