Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A8140_RS14345 Genome accession   NZ_CP015863
Coordinates   2987440..2989188 (-) Length   582 a.a.
NCBI ID   WP_038863028.1    Uniprot ID   -
Organism   Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982440..2994188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8140_RS14325 (A8140_13655) - 2983145..2983984 (-) 840 WP_005532700.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  A8140_RS14330 (A8140_13660) - 2984054..2985547 (-) 1494 WP_005532703.1 SPOR domain-containing protein -
  A8140_RS14335 (A8140_13665) aroB 2985601..2986701 (-) 1101 WP_005532705.1 3-dehydroquinate synthase -
  A8140_RS14340 (A8140_13670) aroK 2986733..2987251 (-) 519 WP_010443904.1 shikimate kinase AroK -
  A8140_RS14345 (A8140_13675) pilQ 2987440..2989188 (-) 1749 WP_038863028.1 type IV pilus secretin PilQ Machinery gene
  A8140_RS14350 (A8140_13680) pilP 2989223..2989738 (-) 516 WP_005532708.1 pilus assembly protein PilP Machinery gene
  A8140_RS14355 (A8140_13685) pilO 2989728..2990321 (-) 594 WP_005532711.1 type 4a pilus biogenesis protein PilO Machinery gene
  A8140_RS14360 (A8140_13690) pilN 2990314..2990889 (-) 576 WP_005532712.1 PilN domain-containing protein Machinery gene
  A8140_RS14365 (A8140_13695) pilM 2990873..2991889 (-) 1017 WP_005532714.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63834.75 Da        Isoelectric Point: 4.5127

>NTDB_id=182605 A8140_RS14345 WP_038863028.1 2987440..2989188(-) (pilQ) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
MKMRHGLTLTPRKLTGLFYLWFSLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKALDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRFDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFH
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=182605 A8140_RS14345 WP_038863028.1 2987440..2989188(-) (pilQ) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTATTTATGGTTCTCTCTACTTTTAGC
CCCTTTTGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCAATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCTGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTTGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCCTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTCGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGAGAGGGCAACATCAGTATGCTTTCAGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCACGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGACG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGATGACTTTCTCAACGTTAACCTTGCCGCGACCAGTCCAAATGCG
ACCAGTATAGCCTTCCAAGTTGCTAAGTTAGGTTCGGACACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
GAAAGCAGAAATTATTTCTAGCCCACGTTTGATAACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAG
ATTACGCCTGACAACCGTTTGGTGTTGGATTTGAGTGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAGGCGGTGGCGATAGATACGCAACGTATTGGTACACAGGTTTTAGTTAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCAT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.625

100

0.986
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.385

97.251

0.753
  pilQ Vibrio cholerae strain A1552

77.385

97.251

0.753
  pilQ Legionella pneumophila strain Lp02

41.379

94.674

0.392
  pilQ Legionella pneumophila strain ERS1305867

41.379

94.674

0.392
  pilQ Pseudomonas aeruginosa PAK

39.964

96.735

0.387
  pilQ Acinetobacter baumannii D1279779

36.772

100

0.38
  pilQ Acinetobacter baumannii strain A118

36.772

100

0.38
  comQ Acinetobacter baylyi ADP1

36.833

99.828

0.368

Multiple sequence alignment