Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   A8140_RS14360 Genome accession   NZ_CP015863
Coordinates   2990314..2990889 (-) Length   191 a.a.
NCBI ID   WP_005532712.1    Uniprot ID   -
Organism   Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2985314..2995889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8140_RS14335 (A8140_13665) aroB 2985601..2986701 (-) 1101 WP_005532705.1 3-dehydroquinate synthase -
  A8140_RS14340 (A8140_13670) aroK 2986733..2987251 (-) 519 WP_010443904.1 shikimate kinase AroK -
  A8140_RS14345 (A8140_13675) pilQ 2987440..2989188 (-) 1749 WP_038863028.1 type IV pilus secretin PilQ Machinery gene
  A8140_RS14350 (A8140_13680) pilP 2989223..2989738 (-) 516 WP_005532708.1 pilus assembly protein PilP Machinery gene
  A8140_RS14355 (A8140_13685) pilO 2989728..2990321 (-) 594 WP_005532711.1 type 4a pilus biogenesis protein PilO Machinery gene
  A8140_RS14360 (A8140_13690) pilN 2990314..2990889 (-) 576 WP_005532712.1 PilN domain-containing protein Machinery gene
  A8140_RS14365 (A8140_13695) pilM 2990873..2991889 (-) 1017 WP_005532714.1 type IV pilus assembly protein PilM Machinery gene
  A8140_RS14370 (A8140_13700) - 2992068..2994635 (+) 2568 WP_005532716.1 penicillin-binding protein 1A -
  A8140_RS14375 (A8140_13705) oxyR 2994714..2995622 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22185.51 Da        Isoelectric Point: 9.1073

>NTDB_id=182608 A8140_RS14360 WP_005532712.1 2990314..2990889(-) (pilN) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
MLYHINLLAWRENQREEHRRRFIGLILLGAIAALGVQWGIGKYYQYQQDQQQQRFDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKTVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTSRLATMLDNMERSAKLLDVDMHSIV
HGKALFGKEFQTFKVSFMFKDASIKGGKQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=182608 A8140_RS14360 WP_005532712.1 2990314..2990889(-) (pilN) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
ATGCTGTATCACATTAATCTTCTTGCTTGGCGTGAAAACCAACGTGAAGAGCATCGTCGTCGTTTTATTGGCTTGATTTT
ACTCGGGGCGATCGCTGCTCTGGGCGTGCAATGGGGGATCGGTAAGTACTACCAATACCAACAAGATCAGCAGCAACAGC
GCTTCGATTATCTTGCTCACTATATCTCTCAACTCGATAAACGTATTGAAGCCATGAAGATAGCAGAGCAAGAGCACAGC
AAAATTCTAGAGCGCTTAAAGACCGTTGAGGGGCTGCAAAATGGGCGCAATAAAACCACTGAGTTTATGAACCTGATGCC
CTCAGTGATCCCTGAAGGCGTCTATGTCGATAAGATCAAAATGAACGACTACGAGATTGAGATCTCGGGTATTTCTGATA
GCACCTCTCGTCTTGCCACCATGCTCGATAATATGGAGCGTTCTGCCAAGCTTCTGGATGTTGATATGCACTCCATTGTC
CATGGTAAAGCGCTCTTTGGTAAAGAGTTCCAGACGTTCAAAGTCTCCTTTATGTTTAAAGATGCCTCTATAAAAGGAGG
TAAGCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

98.429

100

0.984
  pilN Vibrio cholerae strain A1552

59.887

92.67

0.555

Multiple sequence alignment