Detailed information NTG0182609

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A8140_RS14365
• Genome accession: NZ_CP015863
• Coordinates: 2990873..2991889 (-)
• Length: 338 a.a.
• NCBI ID: WP_005532714.1
• Uniprot ID: -
• Organism: Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2985873..2996889

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A8140_RS14340 (A8140_13670)	aroK	2986733..2987251 (-)	519	WP_010443904.1	shikimate kinase AroK	-
A8140_RS14345 (A8140_13675)	pilQ	2987440..2989188 (-)	1749	WP_038863028.1	type IV pilus secretin PilQ	Machinery gene
A8140_RS14350 (A8140_13680)	pilP	2989223..2989738 (-)	516	WP_005532708.1	pilus assembly protein PilP	Machinery gene
A8140_RS14355 (A8140_13685)	pilO	2989728..2990321 (-)	594	WP_005532711.1	type 4a pilus biogenesis protein PilO	Machinery gene
A8140_RS14360 (A8140_13690)	pilN	2990314..2990889 (-)	576	WP_005532712.1	PilN domain-containing protein	Machinery gene
A8140_RS14365 (A8140_13695)	pilM	2990873..2991889 (-)	1017	WP_005532714.1	type IV pilus assembly protein PilM	Machinery gene
A8140_RS14370 (A8140_13700)	-	2992068..2994635 (+)	2568	WP_005532716.1	penicillin-binding protein 1A	-
A8140_RS14375 (A8140_13705)	oxyR	2994714..2995622 (-)	909	WP_005432705.1	DNA-binding transcriptional regulator OxyR	-
A8140_RS14380 (A8140_13710)	-	2995797..2996525 (+)	729	WP_005532719.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37829.28 Da
• Isoelectric Point: 6.5055

>NTDB_id=182609 A8140_RS14365 WP_005532714.1 2990873..2991889(-) (pilM) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGNDSYQVFATRKGVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATDQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGVATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide

• Length: 1017 bp

>NTDB_id=182609 A8140_RS14365 WP_005532714.1 2990873..2991889(-) (pilM) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAGTCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCAATGACA
GTTATCAGGTCTTCGCGACTCGTAAAGGCGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTAGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAATAGTTCTTTCACGATGTTCACCGAACAAGGCGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGATCAATTTAAT
CGACAAATCGTCGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATAAAAGGCGTTTG
GCTTTCGGGAGAGGGCGTTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCGCTTGGTTTGTTTGAAATGAAGGTGTCTAAGCGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	97.633	100	0.976
pilM	Vibrio cholerae strain A1552	48.538	100	0.491