Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A8L59_RS22425 Genome accession   NZ_CP015852
Coordinates   5019775..5020992 (+) Length   405 a.a.
NCBI ID   WP_064589232.1    Uniprot ID   A0AAC9FYF5
Organism   Pseudomonas koreensis strain CRS05-R5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5014775..5025992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8L59_RS22405 (A8L59_22385) - 5016194..5016490 (+) 297 WP_064589229.1 DUF2845 domain-containing protein -
  A8L59_RS22410 (A8L59_22390) - 5016758..5017114 (-) 357 WP_007959598.1 BON domain-containing protein -
  A8L59_RS22415 (A8L59_22395) - 5017415..5017846 (-) 432 WP_064589230.1 pilin -
  A8L59_RS22420 (A8L59_22400) pilB 5018072..5019772 (+) 1701 WP_064589231.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A8L59_RS22425 (A8L59_22405) pilC 5019775..5020992 (+) 1218 WP_064589232.1 type II secretion system F family protein Machinery gene
  A8L59_RS22430 (A8L59_22410) pilD 5020994..5021866 (+) 873 WP_064589233.1 prepilin peptidase Machinery gene
  A8L59_RS22435 (A8L59_22415) coaE 5021863..5022486 (+) 624 WP_064589234.1 dephospho-CoA kinase -
  A8L59_RS22440 (A8L59_22420) yacG 5022483..5022680 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  A8L59_RS22445 (A8L59_22425) - 5022687..5022902 (-) 216 WP_064382081.1 hypothetical protein -
  A8L59_RS22450 (A8L59_22430) - 5022968..5023657 (-) 690 WP_064589235.1 energy-coupling factor ABC transporter permease -
  A8L59_RS22455 (A8L59_22435) - 5023654..5024121 (-) 468 WP_064590097.1 hypothetical protein -
  A8L59_RS22460 (A8L59_22440) - 5024251..5024877 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -
  A8L59_RS22465 (A8L59_22445) - 5024874..5025401 (+) 528 WP_064589236.1 MOSC domain-containing protein -
  A8L59_RS22470 (A8L59_22450) - 5025453..5025626 (+) 174 WP_064589237.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44046.82 Da        Isoelectric Point: 10.1093

>NTDB_id=182506 A8L59_RS22425 WP_064589232.1 5019775..5020992(+) (pilC) [Pseudomonas koreensis strain CRS05-R5]
MAVKAAKISVYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAMVVTGILLVKVVPQFQAVFSGFGAELPAFTLMVIGISEFMQQWWWAVLGVLVAVFFGTR
RALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVATGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=182506 A8L59_RS22425 WP_064589232.1 5019775..5020992(+) (pilC) [Pseudomonas koreensis strain CRS05-R5]
ATGGCGGTCAAGGCAGCAAAAATCAGTGTCTACGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAGGTTACCGGCGAATT
GAGTGGGCAGAACCCCGCGCTGATCAAGGCCCAACTGCGCAAGCAAGGGATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGAGTTTCGGCAAACGCATCAAGGCCCAGGACATCGCGCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCTGGCGTACCTTTGCTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGACAATCCGGCGATGCGCAAACTGGTGGACGA
GGTGAAGCAGGAAGTGGCCGCCGGCAACAGCTTCGCCACTGCCCTGCGCAAGAAGCCGCAGTATTTCGATGAGTTGTATT
GCAACCTGGTGGATGCCGGCGAGCAATCCGGCGCCCTCGACACGCTGCTTGAGCGGGTCGCGACCTACAAGGAAAAAAGC
GAAGCACTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGACAGCGGTGGTGCTGGTGGCGATGGTCGTCACCGGGAT
TTTGCTGGTGAAGGTGGTGCCGCAGTTTCAGGCGGTGTTCTCAGGCTTCGGTGCGGAACTGCCGGCCTTCACGCTGATGG
TGATCGGCATTTCCGAGTTCATGCAGCAATGGTGGTGGGCGGTGCTTGGCGTACTGGTTGCGGTGTTTTTCGGTACGCGT
CGCGCATTGAAAACATCCCAGGCACTGCGTGACCGCAAAGACACCTGGCTGCTGAAACTGCCGCTGGTAGGCACGCTGAT
GTACAAGTCGGCGGTGGCGCGATTCGCCCGCACCCTGTCGACCACGTTCGCCGCTGGCGTGCCGTTGGTAGAAGCGCTCG
ACTCAGTGGCCGGCGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGCTGCGCATCCGCCAGGACGTCGCCACCGGCATG
CAGCTGAATTTTTCCATGCGCAGCACCGGCGTGTTCCCCAACATGGCGGTGCAGATGACCGCTATCGGCGAGGAGTCCGG
CGCGCTGGATGACATGCTCGACAAAGTCGCCGGTTTTTATGAGGACGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCATTTATCATGGTGGTGCTCGGGGTCATCGTCGGCGGTCTGGTCGTGGCCATGTACTTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baumannii D1279779

59.314

100

0.598
  pilC Acinetobacter baylyi ADP1

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilG Neisseria meningitidis 44/76-A

43.842

100

0.44
  pilG Neisseria gonorrhoeae MS11

43.672

99.506

0.435
  pilC Vibrio cholerae strain A1552

43.687

97.778

0.427
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment