Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A8L59_RS22420 Genome accession   NZ_CP015852
Coordinates   5018072..5019772 (+) Length   566 a.a.
NCBI ID   WP_064589231.1    Uniprot ID   A0AAC9BWP5
Organism   Pseudomonas koreensis strain CRS05-R5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5013072..5024772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8L59_RS22400 (A8L59_22380) - 5013246..5016194 (+) 2949 WP_064589228.1 DUF748 domain-containing protein -
  A8L59_RS22405 (A8L59_22385) - 5016194..5016490 (+) 297 WP_064589229.1 DUF2845 domain-containing protein -
  A8L59_RS22410 (A8L59_22390) - 5016758..5017114 (-) 357 WP_007959598.1 BON domain-containing protein -
  A8L59_RS22415 (A8L59_22395) - 5017415..5017846 (-) 432 WP_064589230.1 pilin -
  A8L59_RS22420 (A8L59_22400) pilB 5018072..5019772 (+) 1701 WP_064589231.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A8L59_RS22425 (A8L59_22405) pilC 5019775..5020992 (+) 1218 WP_064589232.1 type II secretion system F family protein Machinery gene
  A8L59_RS22430 (A8L59_22410) pilD 5020994..5021866 (+) 873 WP_064589233.1 prepilin peptidase Machinery gene
  A8L59_RS22435 (A8L59_22415) coaE 5021863..5022486 (+) 624 WP_064589234.1 dephospho-CoA kinase -
  A8L59_RS22440 (A8L59_22420) yacG 5022483..5022680 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  A8L59_RS22445 (A8L59_22425) - 5022687..5022902 (-) 216 WP_064382081.1 hypothetical protein -
  A8L59_RS22450 (A8L59_22430) - 5022968..5023657 (-) 690 WP_064589235.1 energy-coupling factor ABC transporter permease -
  A8L59_RS22455 (A8L59_22435) - 5023654..5024121 (-) 468 WP_064590097.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62476.41 Da        Isoelectric Point: 5.9441

>NTDB_id=182505 A8L59_RS22420 WP_064589231.1 5018072..5019772(+) (pilB) [Pseudomonas koreensis strain CRS05-R5]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFAP
EQIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=182505 A8L59_RS22420 WP_064589231.1 5018072..5019772(+) (pilB) [Pseudomonas koreensis strain CRS05-R5]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAACTGGTACAGGCTGAACTGTTGACGGAAAAAAGCGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTGAAGAGTTCCCAGGTGG
CTGAAATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTATGGCGGCGCGGCAACAAACTGTTCGTGGGCATTTCCGA
CCCGAGCAATCACCAGGCGATCAACGACATCCAGTTCAGCACCGGGTTAAGCACCGAAGCGATTCTGGTAGAGGACGACA
AGCTCACCGATGCCATAGAGAAGTTCTTCGACACCCACGCCAGCGGACTCGAAGAAATGGCCGATGTCGATCTCGACGGT
GTCGATGTCGAATCCGTCGACGACAGCCGCCAGGACGCCATCGGCGGGCTCGATGCCGATGATGCCCCGGTGGTTCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGCGGTTCCTCCGACCTGCACTTCGAACCCTACGAAAAAAACTTCCGGG
TGCGGGTGCGGACCGACGGCATCCTGCGCGAAGTGGCCAAACCGCCAATCCAGTTGGCCGGCCGGATTGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGTG
CGCAGATCGGCATTGATGCGCTGGGTTACGAGCCGGCGCAGAAAGACCTGTACATGGCGGCGCTCAAGCAACCGCAGGGG
ATGATTCTGGTGACCGGGCCGACCGGCTCGGGGAAAACCGTATCGCTGTACACCGGCCTGAATATCCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTTGCCCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCTGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACTGCCGAGATCGCGATCAAGGCTGCGCAGACCGGGCACCTCGTGTTGTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGACCCGCCTGCACAACATGGGTATCCCCGGCTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATCCCTCGCGAAACACTGATCAAGGAGGGCTTTGCGCCG
GAACAGATCGGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAAGGACGCGTGGGAAT
TTATGAAGTGGTGAAAAACACCCCCGAGCTGCAACGGCTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACAGCCAGA
TGCGTCGCGACGGTTTCGACGATCTGCGCACCTCGGGCCTGCACAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.823

0.581
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.558
  pilB Vibrio cholerae strain A1552

51.237

100

0.512
  pilB Vibrio campbellii strain DS40M4

49.561

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilF Thermus thermophilus HB27

36.918

98.587

0.364

Multiple sequence alignment