Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   NX99_RS07570 Genome accession   NZ_CP015282
Coordinates   1654210..1655151 (+) Length   313 a.a.
NCBI ID   WP_002887018.1    Uniprot ID   A0AAW6D6G7
Organism   Streptococcus salivarius strain ATCC 27945     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1649210..1660151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX99_RS07560 (NX99_07420) rpoC 1649967..1653605 (+) 3639 WP_084915245.1 DNA-directed RNA polymerase subunit beta' -
  NX99_RS07565 (NX99_07425) - 1653768..1654130 (+) 363 WP_002887019.1 DUF1033 family protein -
  NX99_RS07570 (NX99_07430) comGA/cglA/cilD 1654210..1655151 (+) 942 WP_002887018.1 competence type IV pilus ATPase ComGA Machinery gene
  NX99_RS07575 (NX99_07435) comYB 1655033..1656133 (+) 1101 WP_148263031.1 competence type IV pilus assembly protein ComGB Machinery gene
  NX99_RS07580 (NX99_07440) comYC 1656142..1656456 (+) 315 WP_037611125.1 competence type IV pilus major pilin ComGC Machinery gene
  NX99_RS07585 (NX99_07445) comYD 1656416..1656844 (+) 429 WP_253183813.1 competence type IV pilus minor pilin ComGD Machinery gene
  NX99_RS07590 (NX99_07450) comGE 1656876..1657106 (+) 231 WP_021144685.1 competence type IV pilus minor pilin ComGE -
  NX99_RS07595 (NX99_07455) comYF 1657093..1657530 (+) 438 WP_021144684.1 competence type IV pilus minor pilin ComGF Machinery gene
  NX99_RS07600 (NX99_07460) comGG 1657508..1657825 (+) 318 WP_021144683.1 competence type IV pilus minor pilin ComGG -
  NX99_RS07605 (NX99_07465) comYH 1657870..1658826 (+) 957 WP_002885753.1 class I SAM-dependent methyltransferase Machinery gene
  NX99_RS07610 (NX99_07470) - 1658883..1660076 (+) 1194 WP_002885831.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35283.31 Da        Isoelectric Point: 5.7041

>NTDB_id=178233 NX99_RS07570 WP_002887018.1 1654210..1655151(+) (comGA/cglA/cilD) [Streptococcus salivarius strain ATCC 27945]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGQRVSLRLSTVGDYRGLESLVIRVLHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFASENFQSHSSTSWNQQLEGLVKQGYLTEGDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=178233 NX99_RS07570 WP_002887018.1 1654210..1655151(+) (comGA/cglA/cilD) [Streptococcus salivarius strain ATCC 27945]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTACGTCATTCCACGTCA
GGATAATTACGAGCTCTATATGCGAGTTGGCCAGGAGAGGAGATTAATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTAGGTTCCTGTGACTATGAT
TGTGGTGATGGTCAAAGGGTTTCTCTGCGTTTGTCTACCGTTGGGGATTATCGTGGCTTAGAAAGTTTAGTTATTCGTGT
TCTACATTCCGAACGTCGAGAATTAGTGTACTGGAATCAAGGAATCCAGCCTATTATGGATGCTTTGGATTATCGTGGCT
TATATCTCTTTGCAGGTCCCGTTGGTTCTGGAAAGACTACGCTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTGATTTCGATTGAGGATCCTGTGGAAATTAAACAGGATAATGTTTTGCAACTTCAGGTGAATCAAGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCACTGCGTCACCGCCCGGATGTTTTGATTATTGGAGAAATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACGGGAGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAGCGTCTTCTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCCCTTCAAGGGATTGCCTACATGCGTCTGAT
TAAGGGAGGAGGTGTGATTGATTTTGCCAGTGAAAATTTCCAAAGCCACTCGTCAACCAGCTGGAACCAGCAGTTGGAAG
GCTTGGTTAAACAAGGATATCTCACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comYA Streptococcus mutans UA159

65.176

100

0.652
  comYA Streptococcus mutans UA140

65.176

100

0.652
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.897

99.681

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

51.447

99.361

0.511
  comGA Latilactobacillus sakei subsp. sakei 23K

41.697

86.581

0.361

Multiple sequence alignment