Detailed information NTG0178237

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: NX99_RS07595
• Genome accession: NZ_CP015282
• Coordinates: 1657093..1657530 (+)
• Length: 145 a.a.
• NCBI ID: WP_021144684.1
• Uniprot ID: A0AAW6D9H3
• Organism: Streptococcus salivarius strain ATCC 27945
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1652093..1662530

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NX99_RS07565 (NX99_07425)	-	1653768..1654130 (+)	363	WP_002887019.1	DUF1033 family protein	-
NX99_RS07570 (NX99_07430)	comGA/cglA/cilD	1654210..1655151 (+)	942	WP_002887018.1	competence type IV pilus ATPase ComGA	Machinery gene
NX99_RS07575 (NX99_07435)	comYB	1655033..1656133 (+)	1101	WP_148263031.1	competence type IV pilus assembly protein ComGB	Machinery gene
NX99_RS07580 (NX99_07440)	comYC	1656142..1656456 (+)	315	WP_037611125.1	competence type IV pilus major pilin ComGC	Machinery gene
NX99_RS07585 (NX99_07445)	comYD	1656416..1656844 (+)	429	WP_253183813.1	competence type IV pilus minor pilin ComGD	Machinery gene
NX99_RS07590 (NX99_07450)	comGE	1656876..1657106 (+)	231	WP_021144685.1	competence type IV pilus minor pilin ComGE	-
NX99_RS07595 (NX99_07455)	comYF	1657093..1657530 (+)	438	WP_021144684.1	competence type IV pilus minor pilin ComGF	Machinery gene
NX99_RS07600 (NX99_07460)	comGG	1657508..1657825 (+)	318	WP_021144683.1	competence type IV pilus minor pilin ComGG	-
NX99_RS07605 (NX99_07465)	comYH	1657870..1658826 (+)	957	WP_002885753.1	class I SAM-dependent methyltransferase	Machinery gene
NX99_RS07610 (NX99_07470)	-	1658883..1660076 (+)	1194	WP_002885831.1	acetate kinase	-
NX99_RS07615 (NX99_07475)	-	1660327..1660524 (+)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
NX99_RS07620 (NX99_07480)	-	1660536..1661192 (+)	657	WP_084915247.1	CPBP family intramembrane glutamic endopeptidase	-
NX99_RS07625 (NX99_07485)	-	1661272..1661718 (+)	447	WP_084915249.1	CAAX protease	-
NX99_RS07630 (NX99_07490)	-	1661831..1662493 (+)	663	WP_002885821.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16229.70 Da
• Isoelectric Point: 9.0226

>NTDB_id=178237 NX99_RS07595 WP_021144684.1 1657093..1657530(+) (comYF) [Streptococcus salivarius strain ATCC 27945]
MSLNSKVRAFTLLECLVALLVIAGSIQVYQGLTTVLVSNVKQVKQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKSSTISQKSQIVTIQVTLKNGLQRSFVYAFEKTS

Nucleotide

• Length: 438 bp

>NTDB_id=178237 NX99_RS07595 WP_021144684.1 1657093..1657530(+) (comYF) [Streptococcus salivarius strain ATCC 27945]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTAGCTCTATTGGTGATTGCTGGTTCAATTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAACCAAGACTGGCTCCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTACCAACCTAT
GCTTTTTAATGTAAAATCTAGTACGATAAGCCAAAAGAGCCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAAGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	56.738	97.241	0.552
comYF	Streptococcus mutans UA159	56.028	97.241	0.545
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	44.604	95.862	0.428