Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   A3777_RS04420 Genome accession   NZ_CP014949
Coordinates   876955..877230 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain LD33     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 857464..895335 876955..877230 within 0


Gene organization within MGE regions


Location: 857464..895335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3777_RS04275 (A3777_04275) - 857464..858471 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  A3777_RS04280 (A3777_04280) comGG 858600..858953 (-) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  A3777_RS04285 (A3777_04285) comGF 858953..859387 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  A3777_RS04290 (A3777_04290) - 859377..859703 (-) 327 WP_010775953.1 type II secretion system protein -
  A3777_RS04300 (A3777_04300) hemH 860505..861446 (-) 942 WP_002357056.1 ferrochelatase -
  A3777_RS04310 (A3777_04310) - 862162..862434 (-) 273 WP_002370964.1 hypothetical protein -
  A3777_RS04315 (A3777_04315) - 862506..862706 (-) 201 WP_002357053.1 cold-shock protein -
  A3777_RS04320 (A3777_04320) - 863543..864784 (-) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  A3777_RS04325 (A3777_04325) - 864785..864883 (-) 99 Protein_828 phage holin -
  A3777_RS04330 (A3777_04330) - 864882..865939 (-) 1058 Protein_829 phage portal protein -
  A3777_RS04335 (A3777_04335) - 865951..867240 (-) 1290 WP_002403123.1 PBSX family phage terminase large subunit -
  A3777_RS04340 (A3777_04340) terS 867212..868015 (-) 804 WP_002389117.1 phage terminase small subunit -
  A3777_RS04345 (A3777_04345) - 868284..868703 (+) 420 WP_230306680.1 hypothetical protein -
  A3777_RS04360 (A3777_04360) - 869760..870176 (-) 417 WP_062775334.1 ArpU family phage packaging/lysis transcriptional regulator -
  A3777_RS14735 - 871083..871202 (-) 120 Protein_834 RusA family crossover junction endodeoxyribonuclease -
  A3777_RS04370 (A3777_04370) - 871257..871439 (+) 183 WP_002364224.1 YegP family protein -
  A3777_RS04375 (A3777_04375) - 871479..872200 (-) 722 Protein_836 Rha family transcriptional regulator -
  A3777_RS04380 (A3777_04380) - 872239..872556 (-) 318 WP_002364228.1 hypothetical protein -
  A3777_RS04385 (A3777_04385) - 872562..872753 (-) 192 WP_002356998.1 hypothetical protein -
  A3777_RS04390 (A3777_04390) - 873044..873388 (+) 345 WP_002385819.1 helix-turn-helix domain-containing protein -
  A3777_RS04395 (A3777_04395) - 873401..874039 (+) 639 WP_002389292.1 ImmA/IrrE family metallo-endopeptidase -
  A3777_RS04400 (A3777_04400) - 874157..874921 (+) 765 WP_002389030.1 LysM peptidoglycan-binding domain-containing protein -
  A3777_RS04405 (A3777_04405) - 874936..875265 (+) 330 WP_002369911.1 hypothetical protein -
  A3777_RS04410 (A3777_04410) - 875340..876488 (+) 1149 WP_002389015.1 tyrosine-type recombinase/integrase -
  A3777_RS04415 (A3777_04415) comGD 876516..876958 (-) 443 Protein_844 competence type IV pilus minor pilin ComGD -
  A3777_RS04420 (A3777_04420) comGC/cglC 876955..877230 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  A3777_RS04425 (A3777_04425) comGB 877230..878276 (-) 1047 WP_002401325.1 competence type IV pilus assembly protein ComGB -
  A3777_RS04430 (A3777_04430) comGA 878233..879201 (-) 969 WP_002401324.1 competence type IV pilus ATPase ComGA -
  A3777_RS04435 (A3777_04435) - 879441..880769 (-) 1329 WP_002362058.1 APC family permease -
  A3777_RS04440 (A3777_04440) rlmN 881059..882132 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  A3777_RS04445 (A3777_04445) - 882257..884086 (-) 1830 WP_002391458.1 ABC transporter permease -
  A3777_RS04450 (A3777_04450) - 884076..884825 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  A3777_RS04455 (A3777_04455) - 884943..885644 (-) 702 WP_002356983.1 GntR family transcriptional regulator -
  A3777_RS04460 (A3777_04460) - 885775..888198 (-) 2424 WP_002411391.1 DNA translocase FtsK -
  A3777_RS04465 (A3777_04465) - 888512..889723 (-) 1212 WP_002411392.1 NAD(P)/FAD-dependent oxidoreductase -
  A3777_RS04470 (A3777_04470) - 889752..890657 (-) 906 WP_002360016.1 prenyltransferase -
  A3777_RS04475 (A3777_04475) - 890779..891759 (+) 981 WP_002360015.1 polyprenyl synthetase family protein -
  A3777_RS04480 (A3777_04480) cydC 891839..893605 (-) 1767 WP_002411395.1 thiol reductant ABC exporter subunit CydC -
  A3777_RS04485 (A3777_04485) cydD 893602..895335 (-) 1734 WP_002364369.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=175351 A3777_RS04420 WP_002356991.1 876955..877230(-) (comGC/cglC) [Enterococcus faecalis strain LD33]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=175351 A3777_RS04420 WP_002356991.1 876955..877230(-) (comGC/cglC) [Enterococcus faecalis strain LD33]
ATGAAAAAGAAACAAAAATACGCGGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGACAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment