Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PMA3_RS03405 Genome accession   NZ_CP014870
Coordinates   768731..769948 (-) Length   405 a.a.
NCBI ID   WP_064675853.1    Uniprot ID   A0A191YMZ2
Organism   Pseudomonas silesiensis strain A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 763731..774948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA3_RS03370 (PMA3_03365) - 764689..764931 (-) 243 WP_064675846.1 DUF2790 domain-containing protein -
  PMA3_RS03375 (PMA3_03370) - 765053..765985 (+) 933 WP_064675847.1 LysR family transcriptional regulator -
  PMA3_RS03380 (PMA3_03375) - 766062..766751 (+) 690 WP_064675848.1 energy-coupling factor ABC transporter permease -
  PMA3_RS03385 (PMA3_03380) - 766815..767030 (+) 216 WP_064675849.1 hypothetical protein -
  PMA3_RS03390 (PMA3_03385) yacG 767040..767240 (-) 201 WP_064675850.1 DNA gyrase inhibitor YacG -
  PMA3_RS03395 (PMA3_03390) coaE 767237..767860 (-) 624 WP_064675851.1 dephospho-CoA kinase -
  PMA3_RS03400 (PMA3_03395) pilD 767857..768729 (-) 873 WP_064675852.1 prepilin peptidase Machinery gene
  PMA3_RS03405 (PMA3_03400) pilC 768731..769948 (-) 1218 WP_064675853.1 type II secretion system F family protein Machinery gene
  PMA3_RS03410 (PMA3_03405) pilB 769951..771651 (-) 1701 WP_064675854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PMA3_RS03415 (PMA3_03410) pilA 771878..772291 (+) 414 WP_064675855.1 pilin Machinery gene
  PMA3_RS03420 (PMA3_03415) - 772606..772959 (+) 354 WP_064675856.1 BON domain-containing protein -
  PMA3_RS03425 (PMA3_03420) - 773245..773544 (-) 300 WP_420848612.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44260.04 Da        Isoelectric Point: 9.8357

>NTDB_id=174908 PMA3_RS03405 WP_064675853.1 768731..769948(-) (pilC) [Pseudomonas silesiensis strain A3]
MAVKAAKISVYAWEGTDRKGTKMTGELSGQNPALIKAQLRKQGINPGKVRKKSASIFNLGKRIKAHDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFKGFGAELPAFTVMVIGLSEFLQEWWWVLLGALIAAFFGLR
HAFKTSQAFRDRTDTWLLKLPLVGTLMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIRQDVATGM
QLNFSMRSSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGAAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=174908 PMA3_RS03405 WP_064675853.1 768731..769948(-) (pilC) [Pseudomonas silesiensis strain A3]
ATGGCGGTCAAGGCAGCAAAAATCAGCGTGTATGCCTGGGAAGGTACGGACCGGAAAGGCACGAAAATGACCGGCGAACT
CAGCGGTCAGAACCCGGCGCTGATCAAGGCGCAATTGCGTAAACAAGGGATCAATCCCGGCAAGGTGCGCAAGAAATCCG
CCTCGATTTTCAACCTGGGCAAGCGCATCAAGGCCCATGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGTGTGCCGCTGTTGCAATCGTTCGACATCATCGGCGAAGGCTTCGATAACCCGGCCATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCGGCGGGCAACAGCTTTGCCGCGGCGCTGCGCAAAAAACCCCAATATTTCGATGAGCTCTACT
GCAACCTGGTCGATGCCGGCGAACAGGCCGGCGCCCTGGACACCCTGTTGGAGCGAGTAGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCAAAGATCAAGAAAGCCATGACTTACCCGCTCGCCGTGGTGTTCGTCGCGATCATCGTGACCGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCGAGTCGGTGTTCAAGGGGTTTGGCGCCGAACTGCCAGCCTTCACCGTGATGG
TCATCGGCCTCTCGGAATTCTTGCAGGAATGGTGGTGGGTGCTGCTCGGCGCGCTGATAGCGGCATTCTTCGGCCTGCGA
CACGCGTTCAAGACGTCGCAAGCCTTTCGGGACCGGACGGATACCTGGCTGCTGAAACTGCCGTTGGTGGGCACGTTGAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGTACGCTGTCGACCACCTTCGCCGCGGGCGTGCCGTTGGTCGAGGCACTGG
AGTCAGTGGCGGGCGCCACCGGCAACATCGTGTTCAAGCGCGCCGTGCTGCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGCTGAATTTTTCCATGCGCTCCTCCGGCATCTTTCCGAACATGGCGATCCAGATGACGGCCATTGGCGAAGAGTCCGG
GGCGCTGGACGACATGCTCGACAAGGTCGCGAGTTTCTACGAGGACGAAGTGGACAACATGGTCGATAACCTCACCAGCC
TGATGGAACCGTTCATCATGGTGGTACTGGGGGTTGTCGTCGGTGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGCGCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191YMZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.086

100

0.731
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.314

100

0.598
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilC Vibrio cholerae strain A1552

44.388

96.79

0.43
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.542

99.259

0.412
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment