Detailed information NTG0174907

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PMA3_RS03400
• Genome accession: NZ_CP014870
• Coordinates: 767857..768729 (-)
• Length: 290 a.a.
• NCBI ID: WP_064675852.1
• Uniprot ID: A0A191YNA3
• Organism: Pseudomonas silesiensis strain A3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 762857..773729

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PMA3_RS03365 (PMA3_03360)	-	763412..764680 (+)	1269	WP_064675845.1	OprD family porin	-
PMA3_RS03370 (PMA3_03365)	-	764689..764931 (-)	243	WP_064675846.1	DUF2790 domain-containing protein	-
PMA3_RS03375 (PMA3_03370)	-	765053..765985 (+)	933	WP_064675847.1	LysR family transcriptional regulator	-
PMA3_RS03380 (PMA3_03375)	-	766062..766751 (+)	690	WP_064675848.1	energy-coupling factor ABC transporter permease	-
PMA3_RS03385 (PMA3_03380)	-	766815..767030 (+)	216	WP_064675849.1	hypothetical protein	-
PMA3_RS03390 (PMA3_03385)	yacG	767040..767240 (-)	201	WP_064675850.1	DNA gyrase inhibitor YacG	-
PMA3_RS03395 (PMA3_03390)	coaE	767237..767860 (-)	624	WP_064675851.1	dephospho-CoA kinase	-
PMA3_RS03400 (PMA3_03395)	pilD	767857..768729 (-)	873	WP_064675852.1	prepilin peptidase	Machinery gene
PMA3_RS03405 (PMA3_03400)	pilC	768731..769948 (-)	1218	WP_064675853.1	type II secretion system F family protein	Machinery gene
PMA3_RS03410 (PMA3_03405)	pilB	769951..771651 (-)	1701	WP_064675854.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PMA3_RS03415 (PMA3_03410)	pilA	771878..772291 (+)	414	WP_064675855.1	pilin	Machinery gene
PMA3_RS03420 (PMA3_03415)	-	772606..772959 (+)	354	WP_064675856.1	BON domain-containing protein	-
PMA3_RS03425 (PMA3_03420)	-	773245..773544 (-)	300	WP_420848612.1	DUF2845 domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32337.60 Da
• Isoelectric Point: 7.9876

>NTDB_id=174907 PMA3_RS03400 WP_064675852.1 767857..768729(-) (pilD) [Pseudomonas silesiensis strain A3]
MPLNDILTVFPLAFVVIAAVVGLLVGSFLNVVIWRLPKMLEREWRLQAHDILGLPEDTPLPTYNLLLPHSQCPHCSHRIR
AWENIPVLSYAFLRGRCSSCKTPISKRYPLTELACGLLSAFIAWHFGFGWPACMVLVLTWGLLAMSLIDAEHQLLPDVLV
LPLMWLGLIINSFGLFAPLQEALWGAIAGYTALWSVFWLFKLVTGKDGMGHGDFKLLAMFGAWGGWQILPLTLLLSSLVG
AVIGVIWLRMRNAQTSTPIPFGPYLAIAGWIALLWGGQITDFYWQFVGLK

Nucleotide

• Length: 873 bp

>NTDB_id=174907 PMA3_RS03400 WP_064675852.1 767857..768729(-) (pilD) [Pseudomonas silesiensis strain A3]
ATGCCCTTGAACGATATTCTGACTGTCTTTCCGCTGGCCTTCGTCGTCATTGCCGCAGTGGTCGGCCTGCTGGTCGGCAG
CTTCCTCAATGTGGTGATCTGGCGCCTGCCAAAAATGCTCGAGCGTGAGTGGCGACTGCAGGCCCATGACATCCTGGGCT
TGCCTGAGGACACGCCCCTGCCGACTTACAACCTGCTGCTGCCCCACTCTCAATGCCCGCACTGCAGCCACCGGATTCGG
GCCTGGGAAAACATTCCAGTGCTCAGTTATGCATTTCTGCGAGGGCGATGTTCAAGCTGCAAGACGCCGATCAGCAAACG
CTACCCGTTGACCGAACTGGCTTGCGGGCTGCTGTCGGCGTTCATCGCCTGGCACTTCGGGTTCGGTTGGCCGGCGTGCA
TGGTGCTCGTCCTGACCTGGGGCCTGTTGGCCATGAGCCTGATCGACGCAGAGCACCAGCTGCTGCCGGATGTGCTGGTA
CTCCCCCTGATGTGGCTCGGATTGATCATCAACAGTTTCGGCCTGTTCGCGCCGCTCCAGGAGGCATTGTGGGGCGCGAT
AGCCGGCTACACGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTCACCGGCAAGGACGGCATGGGCCACGGTGATT
TCAAGCTGTTGGCGATGTTCGGCGCCTGGGGCGGCTGGCAGATCCTGCCACTGACCCTCCTGTTGTCATCCCTGGTGGGC
GCCGTTATCGGCGTGATCTGGCTGCGCATGCGCAACGCGCAAACCTCGACACCGATCCCTTTCGGCCCCTATCTGGCAAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A191YNA3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.632	98.276	0.517
pilD	Vibrio campbellii strain DS40M4	50	97.931	0.49
pilD	Acinetobacter baumannii D1279779	48.252	98.621	0.476
pilD	Neisseria gonorrhoeae MS11	50.37	93.103	0.469
pilD	Acinetobacter nosocomialis M2	47.552	98.621	0.469