Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PMA3_RS03410 Genome accession   NZ_CP014870
Coordinates   769951..771651 (-) Length   566 a.a.
NCBI ID   WP_064675854.1    Uniprot ID   A0A191YNE8
Organism   Pseudomonas silesiensis strain A3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764951..776651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA3_RS03375 (PMA3_03370) - 765053..765985 (+) 933 WP_064675847.1 LysR family transcriptional regulator -
  PMA3_RS03380 (PMA3_03375) - 766062..766751 (+) 690 WP_064675848.1 energy-coupling factor ABC transporter permease -
  PMA3_RS03385 (PMA3_03380) - 766815..767030 (+) 216 WP_064675849.1 hypothetical protein -
  PMA3_RS03390 (PMA3_03385) yacG 767040..767240 (-) 201 WP_064675850.1 DNA gyrase inhibitor YacG -
  PMA3_RS03395 (PMA3_03390) coaE 767237..767860 (-) 624 WP_064675851.1 dephospho-CoA kinase -
  PMA3_RS03400 (PMA3_03395) pilD 767857..768729 (-) 873 WP_064675852.1 prepilin peptidase Machinery gene
  PMA3_RS03405 (PMA3_03400) pilC 768731..769948 (-) 1218 WP_064675853.1 type II secretion system F family protein Machinery gene
  PMA3_RS03410 (PMA3_03405) pilB 769951..771651 (-) 1701 WP_064675854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PMA3_RS03415 (PMA3_03410) pilA 771878..772291 (+) 414 WP_064675855.1 pilin Machinery gene
  PMA3_RS03420 (PMA3_03415) - 772606..772959 (+) 354 WP_064675856.1 BON domain-containing protein -
  PMA3_RS03425 (PMA3_03420) - 773245..773544 (-) 300 WP_420848612.1 DUF2845 domain-containing protein -
  PMA3_RS03430 (PMA3_03425) - 773541..776492 (-) 2952 WP_064675858.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62746.74 Da        Isoelectric Point: 6.1173

>NTDB_id=174909 PMA3_RS03410 WP_064675854.1 769951..771651(-) (pilB) [Pseudomonas silesiensis strain A3]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNRISLVSYLVQNKLVNSRQVAEIASEHFGMALLDLHCLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNQQAINDIQFSTGLSTEAILVEDEKLTDAIEKFFDSHSTGLEDMADVDLDG
VDIESIDDNRQDAVAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKSYRVRMRTDGMLREVAKPPIQLANRIASRL
KVMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLFMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLGFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKRPIEIPREALLKEGFPA
ERIGSFTIYEPVGCDHCNNGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=174909 PMA3_RS03410 WP_064675854.1 769951..771651(-) (pilB) [Pseudomonas silesiensis strain A3]
ATGAATGACATCGCCCTTAGCGGCCTGGCCAAGCAATTGGTCCTGGCTGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAGCGCAACCGGATTTCCCTGGTCAGCTACCTGGTGCAAAACAAACTGGTGAATAGCCGTCAGGTTG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTGGATCTCCACTGCCTGGATAAAGAGTCACAACCCAAAGGGCTG
GTCAGCGAGAAACTGGTCCGCCAGCACCACGCCCTGCCCTTGTGGCGGCGCGGCAACAAACTGTTCGTGGGGGTTTCCGA
CCCGACCAATCAACAGGCCATCAACGACATCCAGTTCAGCACCGGGCTCAGTACCGAAGCCATTCTGGTCGAAGACGAAA
AGCTCACGGACGCCATCGAGAAATTCTTCGACAGCCACAGTACCGGCCTGGAAGACATGGCCGACGTTGACCTCGATGGC
GTGGACATCGAGTCGATCGATGACAACCGTCAGGACGCCGTCGCCGGACAGGATACCGATGACGCGCCCGTGGTGCGCTT
CGTTCACAAGATGTTGCTCGATGCGATCAAAAGCGGTTCCTCCGACCTGCACTTCGAACCCTACGAAAAATCCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGGGAAGTCGCCAAGCCGCCGATTCAATTGGCCAACCGCATCGCTTCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAACGACGCCGGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTTCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTCGGCTACGAGCCCGATCAGAAAGACTTGTTCATGGCCGCACTCAAGCAGCCACAGGGG
ATGATTCTGGTGACAGGGCCCACCGGGTCGGGCAAGACCGTGTCGCTCTACACCGGCCTGAATATCCTCAATACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GCCTGGGATTCGCCCAGGCCCTGCGTTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACGGCGGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTTGTGCTGTCGACCCTGCACACCAACAGCGCTGCGGA
AACCCTGACCCGCCTGCACAATATGGGCATCCAGGGCTTCAACATCGCTACTTCGGTGAGCCTGATCATCGCCCAACGCC
TGGCGCGTAAACTGTGCAGCCATTGCAAGCGACCGATCGAGATTCCCCGGGAGGCGCTACTAAAGGAAGGTTTCCCCGCG
GAACGCATCGGCTCGTTCACGATCTATGAGCCCGTCGGTTGCGATCACTGCAACAACGGTTACAAAGGGCGCGTAGGGAT
TTATGAAGTGGTGAAGAACACTCCCGAGTTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATACCCAGA
TGCGCAAGGACGGGTTCAACGACCTGCGCACTTCCGGGTTGATCAAGGCCATGCAGGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191YNE8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.503

99.47

0.592
  pilB Acinetobacter baumannii D1279779

58.865

99.647

0.587
  pilB Legionella pneumophila strain ERS1305867

55.221

99.823

0.551
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.54

98.233

0.496
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilF Thermus thermophilus HB27

36.572

100

0.366

Multiple sequence alignment