Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CSTERLE_RS05990 Genome accession   NZ_CP014673
Coordinates   1391552..1392610 (+) Length   352 a.a.
NCBI ID   WP_065820716.1    Uniprot ID   A0A1B1YK59
Organism   Thermoclostridium stercorarium subsp. leptospartum DSM 9219     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1386552..1397610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSTERLE_RS05975 (CSTERLE_05975) - 1388081..1389124 (+) 1044 WP_237266954.1 hypothetical protein -
  CSTERLE_RS05980 (CSTERLE_05980) - 1389102..1390328 (-) 1227 WP_015358103.1 IS256 family transposase -
  CSTERLE_RS05985 (CSTERLE_05985) pilF 1390413..1391540 (+) 1128 WP_237266955.1 GspE/PulE family protein Machinery gene
  CSTERLE_RS05990 (CSTERLE_05990) pilT 1391552..1392610 (+) 1059 WP_065820716.1 type IV pilus twitching motility protein PilT Machinery gene
  CSTERLE_RS05995 (CSTERLE_05995) pilM 1392641..1393738 (+) 1098 WP_015358939.1 type IV pilus assembly protein PilM -
  CSTERLE_RS06000 (CSTERLE_06000) - 1393751..1394968 (+) 1218 WP_015358940.1 type II secretion system F family protein -
  CSTERLE_RS06005 (CSTERLE_06005) - 1395075..1395614 (+) 540 WP_065820717.1 type II secretion system protein -
  CSTERLE_RS06010 (CSTERLE_06010) - 1395759..1396178 (+) 420 WP_015358942.1 hypothetical protein -
  CSTERLE_RS14450 - 1396274..1396468 (+) 195 WP_237266957.1 hypothetical protein -
  CSTERLE_RS06015 (CSTERLE_06015) - 1396452..1396835 (+) 384 WP_237266959.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38858.78 Da        Isoelectric Point: 6.6322

>NTDB_id=173231 CSTERLE_RS05990 WP_065820716.1 1391552..1392610(+) (pilT) [Thermoclostridium stercorarium subsp. leptospartum DSM 9219]
MNINEYLQKAVEMDASDLHICAGLPVVIRKHGSLFFVDDHILTGEDCLSLVKQLLNDSQFAFLEEEGEIDFAYMLSGVGR
FRVNAFRQRGTYAIAIRVIPSRVPKISELGLPDILYSLAERQRGLVLITGPTGSGKSTTLAAMIDHINSTKRCHIVTIED
PIEYLHKHNKSVINQREIGSDTKSYSNALRAVLREDPDVILIGEMRDLETISIALTAAETGHLVLSTLHTLGAAKTVDRI
IDVFPPHQQGQVRSQLSTVLQGIVSQQLIKKADNAGRVVATEVMIVTPAIANMIRESRTYQINSSIHTGGEYGMHSMDAC
IASLYKRGLITYENACQYAMDPVAMKKLIALE

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=173231 CSTERLE_RS05990 WP_065820716.1 1391552..1392610(+) (pilT) [Thermoclostridium stercorarium subsp. leptospartum DSM 9219]
ATGAATATCAACGAATACTTGCAAAAAGCCGTAGAGATGGATGCTTCAGACCTTCATATTTGTGCAGGTTTACCGGTGGT
CATAAGAAAACACGGCTCGCTGTTTTTTGTCGATGACCACATACTTACAGGAGAAGATTGCCTGAGTTTGGTGAAGCAGT
TGTTGAACGACTCGCAGTTCGCGTTTCTTGAAGAAGAAGGAGAAATTGACTTTGCTTATATGCTCTCCGGTGTCGGAAGA
TTCAGGGTCAATGCTTTCAGGCAAAGAGGAACTTATGCCATAGCCATAAGGGTTATTCCGTCGAGAGTACCGAAAATCAG
TGAATTGGGGCTTCCGGATATCCTTTATTCACTGGCGGAAAGGCAGAGAGGCCTTGTCCTTATAACAGGGCCTACCGGAA
GCGGTAAATCCACAACCCTTGCCGCAATGATAGATCATATTAACAGTACAAAAAGATGCCACATTGTAACCATTGAAGAT
CCTATCGAATATTTGCATAAACACAACAAAAGCGTGATAAATCAGCGTGAAATAGGCTCGGATACTAAAAGTTACAGCAA
CGCATTGAGAGCAGTGTTAAGAGAAGATCCCGACGTTATTCTGATTGGTGAGATGAGAGATTTGGAGACAATTTCCATTG
CGTTAACCGCTGCTGAAACGGGACATCTGGTTCTGTCAACACTGCATACATTGGGAGCGGCCAAGACTGTTGACAGAATT
ATAGACGTATTCCCTCCGCATCAGCAAGGGCAGGTACGCAGCCAGCTGTCGACGGTGCTACAGGGGATTGTTTCGCAGCA
GTTGATAAAGAAAGCTGATAATGCGGGAAGAGTGGTCGCTACAGAGGTCATGATAGTAACTCCTGCGATAGCAAACATGA
TAAGGGAGTCGAGGACGTATCAGATCAACAGCAGCATTCATACGGGCGGAGAATACGGCATGCATTCCATGGATGCGTGC
ATAGCTTCTTTATATAAACGCGGTTTGATTACTTATGAAAATGCATGCCAGTATGCCATGGACCCTGTGGCAATGAAAAA
GTTAATAGCGCTGGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.047

97.159

0.506
  pilT Neisseria meningitidis 8013

50.867

98.295

0.5
  pilT Neisseria gonorrhoeae MS11

50.578

98.295

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.864

0.494
  pilT Pseudomonas stutzeri DSM 10701

50.585

97.159

0.491
  pilT Acinetobacter baumannii D1279779

50

97.159

0.486
  pilT Acinetobacter nosocomialis M2

50

97.159

0.486
  pilT Acinetobacter baumannii strain A118

50

97.159

0.486
  pilT Acinetobacter baylyi ADP1

49.415

97.159

0.48
  pilT Vibrio cholerae strain A1552

51.057

94.034

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.057

94.034

0.48
  pilT Legionella pneumophila strain ERS1305867

49.267

96.875

0.477
  pilT Legionella pneumophila strain Lp02

49.267

96.875

0.477
  pilU Pseudomonas stutzeri DSM 10701

41.595

99.716

0.415
  pilU Acinetobacter baylyi ADP1

39.773

100

0.398
  pilU Vibrio cholerae strain A1552

39.528

96.307

0.381

Multiple sequence alignment