Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PcP3B5_RS05275 Genome accession   NZ_CP014158
Coordinates   1142213..1143430 (-) Length   405 a.a.
NCBI ID   WP_061560724.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain P3B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1140452..1150769 1142213..1143430 within 0


Gene organization within MGE regions


Location: 1140452..1150769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PcP3B5_RS05260 (PcP3B5_10720) yacG 1140452..1140661 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  PcP3B5_RS05265 (PcP3B5_10730) coaE 1140658..1141266 (-) 609 WP_009617624.1 dephospho-CoA kinase -
  PcP3B5_RS05270 (PcP3B5_10740) pilD 1141340..1142212 (-) 873 WP_061560723.1 prepilin peptidase Machinery gene
  PcP3B5_RS05275 (PcP3B5_10750) pilC 1142213..1143430 (-) 1218 WP_061560724.1 type II secretion system F family protein Machinery gene
  PcP3B5_RS05280 (PcP3B5_10760) pilB 1143434..1145137 (-) 1704 WP_061560725.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PcP3B5_RS30525 pilA/pilAI 1145367..1145786 (+) 420 WP_082816254.1 pilin Machinery gene
  PcP3B5_RS30530 (PcP3B5_10770) - 1145951..1147945 (+) 1995 WP_145923854.1 sulfatase-like hydrolase/transferase -
  PcP3B5_RS05295 (PcP3B5_10790) - 1148321..1149325 (+) 1005 WP_420820162.1 diguanylate cyclase -
  PcP3B5_RS05300 (PcP3B5_10800) - 1149322..1150518 (-) 1197 WP_061560728.1 type II toxin-antitoxin system HipA family toxin -
  PcP3B5_RS05305 (PcP3B5_10810) - 1150518..1150769 (-) 252 WP_043318092.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43997.80 Da        Isoelectric Point: 10.0333

>NTDB_id=168923 PcP3B5_RS05275 WP_061560724.1 1142213..1143430(-) (pilC) [Pseudomonas citronellolis strain P3B5]
MAEKALKMSVFTWEGTDRKGGKVKGELSGTNTALVKAQLRKQGINPTKVRKKGISLLGKGKRIKPLDIALFTRQMATMMG
SGVPLLQSFDIIAEGFDNPNMRKLVEDIKQEVAAGNSLANSLRRKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVALVVSAILLIKVVPQFQSVFAGFGAELPAFTKMVINLSEVLQKWWIIVLIALFIIAFALK
EAHKRSEKFRDSVDRGILKLPIVGGIIYKSAVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKIKQDVSSGT
QLNFSMRTTGAFPTLAIQMAAIGEESGSLDAMLDKVATYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=168923 PcP3B5_RS05275 WP_061560724.1 1142213..1143430(-) (pilC) [Pseudomonas citronellolis strain P3B5]
ATGGCGGAAAAAGCACTGAAGATGAGCGTCTTTACCTGGGAGGGTACGGATCGCAAGGGCGGGAAGGTAAAAGGGGAGCT
TTCCGGCACCAATACCGCACTGGTCAAGGCACAACTGCGCAAGCAGGGCATCAACCCGACCAAGGTACGCAAAAAGGGTA
TTTCGCTACTGGGCAAGGGCAAGCGAATCAAGCCCCTGGATATCGCTCTCTTTACCCGGCAGATGGCAACCATGATGGGC
TCGGGGGTTCCGCTCCTGCAATCCTTCGACATCATTGCCGAGGGCTTCGACAACCCCAACATGCGCAAGCTGGTCGAGGA
CATCAAGCAGGAGGTCGCTGCCGGTAACAGCCTCGCCAACTCGCTGCGCAGGAAGCCGCAATACTTCGACGACCTCTACT
GCAACCTTGTCGATGCTGGTGAACAGTCCGGCGCACTGGAAACGCTGCTGGACCGCGTCGCCACCTACAAGGAAAAAACC
GAGTCGCTGAAAGCCAAGATCAAGAAAGCGATGACCTACCCCATCGCAGTCATCATCGTGGCACTTGTAGTTTCGGCAAT
CCTGCTGATCAAAGTCGTCCCCCAGTTCCAATCGGTGTTCGCAGGTTTCGGAGCCGAACTGCCCGCCTTTACCAAGATGG
TCATCAACTTGTCAGAAGTGCTCCAGAAATGGTGGATTATTGTCCTGATAGCACTATTTATAATTGCCTTCGCCCTGAAG
GAGGCGCATAAACGATCGGAGAAATTCCGCGACTCTGTTGACCGCGGCATTCTGAAACTACCGATCGTAGGCGGCATTAT
CTACAAGTCCGCTGTTGCCCGGTATGCACGCACGCTAGCAACCACCTTCGCAGCAGGCGTTCCCTTGGTGGAGGCGCTGG
ACTCGGTTGCCGGCGCTACTGGCAACGTCGTTTTTCGTAATGCTGTGAACAAAATCAAGCAAGACGTCTCTTCGGGTACT
CAACTCAACTTTTCCATGCGTACCACTGGAGCGTTCCCCACGCTGGCAATACAAATGGCCGCCATAGGTGAGGAATCCGG
CTCGCTGGACGCAATGCTGGACAAAGTGGCCACCTACTACGAAGAAGAAGTCGATAATGCCGTCGACAACCTCACCACTC
TGATGGAGCCGATGATCATGGCCGTTCTTGGTGTATTGGTAGGCGGCTTGGTCATCGCCATGTACCTCCCTATCTTCCAA
CTCGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.012

100

0.79
  pilC Acinetobacter baumannii D1279779

61.481

100

0.615
  pilC Acinetobacter baylyi ADP1

60.345

100

0.605
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

44.279

99.259

0.44
  pilG Neisseria meningitidis 44/76-A

44.697

97.778

0.437
  pilC Vibrio cholerae strain A1552

43.21

100

0.432
  pilC Vibrio campbellii strain DS40M4

41.667

97.778

0.407
  pilC Thermus thermophilus HB27

36.973

99.506

0.368

Multiple sequence alignment