Detailed information NTG0168922

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PcP3B5_RS05270
• Genome accession: NZ_CP014158
• Coordinates: 1141340..1142212 (-)
• Length: 290 a.a.
• NCBI ID: WP_061560723.1
• Uniprot ID: -
• Organism: Pseudomonas citronellolis strain P3B5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1140452..1150769	1141340..1142212	within	0

Gene organization within MGE regions

Location: 1140452..1150769

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PcP3B5_RS05260 (PcP3B5_10720)	yacG	1140452..1140661 (-)	210	WP_043272268.1	DNA gyrase inhibitor YacG	-
PcP3B5_RS05265 (PcP3B5_10730)	coaE	1140658..1141266 (-)	609	WP_009617624.1	dephospho-CoA kinase	-
PcP3B5_RS05270 (PcP3B5_10740)	pilD	1141340..1142212 (-)	873	WP_061560723.1	prepilin peptidase	Machinery gene
PcP3B5_RS05275 (PcP3B5_10750)	pilC	1142213..1143430 (-)	1218	WP_061560724.1	type II secretion system F family protein	Machinery gene
PcP3B5_RS05280 (PcP3B5_10760)	pilB	1143434..1145137 (-)	1704	WP_061560725.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PcP3B5_RS30525	pilA/pilAI	1145367..1145786 (+)	420	WP_082816254.1	pilin	Machinery gene
PcP3B5_RS30530 (PcP3B5_10770)	-	1145951..1147945 (+)	1995	WP_145923854.1	sulfatase-like hydrolase/transferase	-
PcP3B5_RS05295 (PcP3B5_10790)	-	1148321..1149325 (+)	1005	WP_420820162.1	diguanylate cyclase	-
PcP3B5_RS05300 (PcP3B5_10800)	-	1149322..1150518 (-)	1197	WP_061560728.1	type II toxin-antitoxin system HipA family toxin	-
PcP3B5_RS05305 (PcP3B5_10810)	-	1150518..1150769 (-)	252	WP_043318092.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31976.97 Da
• Isoelectric Point: 7.3451

>NTDB_id=168922 PcP3B5_RS05270 WP_061560723.1 1141340..1142212(-) (pilD) [Pseudomonas citronellolis strain P3B5]
MPVLDFLASHALAFVLCAALLGLLIGSFLNVVIHRLPKMMELEWQQQARDALSLPQPEKKATYNLVLPNSQCPHCGHEIR
AWENIPVVSYLALRGKCSACKAPISARYPLVELATALLSGFVAWHFGFTWQAGAMLLLTWGLLAMSLIDVDHQLLPDALV
LPLLWLGLIVNYFGLFTSLGDALWGAVFGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILFSSLVG
AVLGVILLRLRNAETSTPIPFGPYLAIAGWIALLWGDQITTTYLQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=168922 PcP3B5_RS05270 WP_061560723.1 1141340..1142212(-) (pilD) [Pseudomonas citronellolis strain P3B5]
ATGCCTGTACTCGACTTCCTGGCCAGCCACGCGCTGGCCTTTGTTTTATGCGCCGCCCTACTCGGCCTGCTGATTGGCAG
CTTCCTCAACGTAGTTATCCACCGCCTGCCAAAGATGATGGAACTGGAGTGGCAGCAACAAGCGCGTGATGCGCTTTCCC
TGCCCCAACCCGAGAAAAAGGCCACCTACAACCTGGTTCTGCCCAACTCCCAGTGCCCGCATTGCGGTCATGAAATCCGT
GCCTGGGAGAACATCCCCGTAGTCAGCTACCTGGCCCTACGCGGCAAGTGCTCGGCCTGCAAGGCGCCGATCAGTGCGCG
TTATCCGCTGGTCGAGCTGGCCACCGCTTTGCTTTCCGGTTTCGTTGCCTGGCATTTCGGCTTTACCTGGCAGGCCGGGG
CCATGCTGTTGCTGACCTGGGGGCTGCTGGCCATGAGCCTGATCGACGTCGATCACCAGCTACTGCCGGACGCCTTGGTG
CTGCCGTTGCTCTGGCTGGGGTTGATCGTCAATTACTTCGGGCTTTTCACCAGCCTGGGCGATGCGCTCTGGGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGCTGCTGGCCATGCTCGGCGCCTGGGGCGGCTGGCAGGTGTTGCCGCTGACCATTCTCTTCTCGTCCCTGGTCGGG
GCCGTGCTGGGCGTCATTCTGCTGCGTCTGCGCAATGCCGAGACCAGCACGCCGATCCCCTTCGGGCCCTATCTGGCCAT
CGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACCACTACCTATCTGCAGTTCGCCGGTTTCCGGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	58.484	95.517	0.559
pilD	Vibrio campbellii strain DS40M4	56.41	94.138	0.531
pilD	Acinetobacter baumannii D1279779	53.684	98.276	0.528
pilD	Acinetobacter nosocomialis M2	53.004	97.586	0.517
pilD	Neisseria gonorrhoeae MS11	50.57	90.69	0.459