Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PcP3B5_RS05280 Genome accession   NZ_CP014158
Coordinates   1143434..1145137 (-) Length   567 a.a.
NCBI ID   WP_061560725.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain P3B5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1140452..1150769 1143434..1145137 within 0


Gene organization within MGE regions


Location: 1140452..1150769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PcP3B5_RS05260 (PcP3B5_10720) yacG 1140452..1140661 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  PcP3B5_RS05265 (PcP3B5_10730) coaE 1140658..1141266 (-) 609 WP_009617624.1 dephospho-CoA kinase -
  PcP3B5_RS05270 (PcP3B5_10740) pilD 1141340..1142212 (-) 873 WP_061560723.1 prepilin peptidase Machinery gene
  PcP3B5_RS05275 (PcP3B5_10750) pilC 1142213..1143430 (-) 1218 WP_061560724.1 type II secretion system F family protein Machinery gene
  PcP3B5_RS05280 (PcP3B5_10760) pilB 1143434..1145137 (-) 1704 WP_061560725.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PcP3B5_RS30525 pilA/pilAI 1145367..1145786 (+) 420 WP_082816254.1 pilin Machinery gene
  PcP3B5_RS30530 (PcP3B5_10770) - 1145951..1147945 (+) 1995 WP_145923854.1 sulfatase-like hydrolase/transferase -
  PcP3B5_RS05295 (PcP3B5_10790) - 1148321..1149325 (+) 1005 WP_420820162.1 diguanylate cyclase -
  PcP3B5_RS05300 (PcP3B5_10800) - 1149322..1150518 (-) 1197 WP_061560728.1 type II toxin-antitoxin system HipA family toxin -
  PcP3B5_RS05305 (PcP3B5_10810) - 1150518..1150769 (-) 252 WP_043318092.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62598.52 Da        Isoelectric Point: 5.2374

>NTDB_id=168924 PcP3B5_RS05280 WP_061560725.1 1143434..1145137(-) (pilB) [Pseudomonas citronellolis strain P3B5]
MNDQVVLSGLARQLVIAELLDERSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAFCDLTAIDKDGIPKE
LISEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGDTIEKLFESATGGLDDLADVDLD
GLDIESGDADRKEESIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEAAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDSLGYDEAQKELYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDIPNETLLHEGFP
EDQVGSFKLYSPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=168924 PcP3B5_RS05280 WP_061560725.1 1143434..1145137(-) (pilB) [Pseudomonas citronellolis strain P3B5]
ATGAACGATCAAGTCGTGCTATCCGGCCTCGCCCGTCAGTTGGTCATCGCCGAGCTACTGGACGAAAGAAGCGCCCAGCA
GGCCCAGCAGCAGGCTCAACGCAACAAACTTTCGCTGGTGACCTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGGCCTC
TGACCGAGCTAGCCGCCGATCAGTTCGGTGTCGCCTTCTGCGACCTGACGGCCATCGACAAGGACGGCATTCCAAAGGAA
TTGATCAGCGAGAAGCTGGTGCGTCAGCATCGCGTCATTCCACTCTGGCGGCGCGGCAACAAGCTATTCGTAGGTATATC
TGATCCCACGAATCATCAAGCCATCACCGATGTTCAGTTCAGTACCGGCCTGACCACCGAGGCGATTCTGGTCGAGGACG
ACAAGCTCGGCGACACCATCGAAAAACTCTTCGAAAGTGCCACCGGCGGCCTGGACGATCTGGCCGATGTCGATCTGGAT
GGGCTGGATATTGAAAGTGGTGACGCAGACCGCAAGGAAGAAAGCATTGGAGGCGATGCTGACGACGCTCCGGTAGTCCG
CTTCGTCAACAAGATGCTACTGGACGCTATCAAGGGAGGTTCATCGGACCTGCACTTCGAGCCCTACGAGAAGATCTACC
GCGTACGCTTCCGTACCGACGGCATGCTGCATGAGGCGGCCAAGCCTCCTATCCAACTGGCTAGCCGTATCTCGGCGCGC
CTAAAGGTGATGGCTGGCCTGGATATTTCCGAAAGGCGCAAGCCTCAGGACGGCCGCATCAAGATGCGGGTGTCCAAGAA
CAAATCCATCGATTTCCGCGTCAACACCCTACCTACTCTGTGGGGGGAGAAAATCGTGATGCGGATCCTCGACTCGGCCA
GCGCGCAAATGGGGATCGACTCGCTCGGCTACGACGAGGCTCAGAAGGAACTCTACCTGGAGGCGCTCAAGCAACCTCAG
GGCATGATCCTCGTGACCGGCCCGACCGGCTCCGGCAAGACCGTATCCTTATATACCGGCCTGAATATCCTCAATACTCC
GGACATCAATATTTCCACCGCCGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAAGTCAACGTCAACCCTCGCC
AGGGCATGGACTTCGCCCAGGCGTTACGTGCTTTCCTGCGCCAGGATCCAGACGTAATCATGGTCGGCGAGATTCGCGAC
CTGGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGCCATATGGTGATGTCCACGCTGCACACCAATAGCGCTGC
GGAAACCCTGACGCGCCTGCTGAACATGGGCGTGGCCTCCTTCAACCTGGCTACCTCCACCAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTCTGCCCGCACTGCAAGAAGGAACACGACATCCCCAACGAAACCCTCCTCCACGAAGGCTTCCCC
GAGGACCAGGTCGGCAGCTTCAAGCTGTACTCCCCCGTGGGGTGTGAAAATTGCAAGAACGGCTACAAGGGCCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAAGAAGGCTTCAATGACCTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.699

99.647

0.575
  pilB Acinetobacter baylyi ADP1

57.371

99.295

0.57
  pilB Legionella pneumophila strain ERS1305867

54.69

99.647

0.545
  pilB Vibrio cholerae strain A1552

50.267

98.942

0.497
  pilB Vibrio campbellii strain DS40M4

49.558

99.647

0.494
  pilF Neisseria gonorrhoeae MS11

49.556

99.295

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

51.197

95.767

0.49
  pilF Thermus thermophilus HB27

38.947

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.298

0.369

Multiple sequence alignment