Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL543_RS07645 Genome accession   NZ_CP014043
Coordinates   198072..199760 (+) Length   562 a.a.
NCBI ID   WP_061051580.1    Uniprot ID   -
Organism   Vibrio mimicus strain FDAARGOS_112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 193072..204760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL543_RS07615 (AL543_06885) dsbC 193350..194102 (-) 753 WP_061051575.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  AL543_RS07620 (AL543_06890) xerD 194119..194997 (-) 879 WP_188318481.1 site-specific tyrosine recombinase XerD -
  AL543_RS07625 (AL543_06895) fldB 195165..195683 (+) 519 WP_061051576.1 flavodoxin FldB -
  AL543_RS07630 (AL543_06900) ampD 195741..196289 (-) 549 WP_061051577.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL543_RS07635 (AL543_06905) nadC 196467..197357 (+) 891 WP_061051578.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL543_RS07640 (AL543_06910) pilA 197602..198072 (+) 471 WP_061051579.1 pilin Machinery gene
  AL543_RS07645 (AL543_06915) pilB 198072..199760 (+) 1689 WP_061051580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL543_RS07650 (AL543_06920) pilC 199799..201025 (+) 1227 WP_061051581.1 type II secretion system F family protein Machinery gene
  AL543_RS07655 (AL543_06925) pilD 201088..201963 (+) 876 WP_061051582.1 prepilin peptidase Machinery gene
  AL543_RS07660 (AL543_06930) coaE 201960..202568 (+) 609 WP_061051583.1 dephospho-CoA kinase -
  AL543_RS07665 (AL543_06935) zapD 202600..203340 (+) 741 WP_061051584.1 cell division protein ZapD -
  AL543_RS07670 (AL543_06940) yacG 203591..203788 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62222.13 Da        Isoelectric Point: 5.7044

>NTDB_id=167865 AL543_RS07645 WP_061051580.1 198072..199760(+) (pilB) [Vibrio mimicus strain FDAARGOS_112]
MLTNLVAILRQAELISSTQEQAVVEQVSASGTSVPEALLELSIFHAQELTEQISHIFGLPETDLSHYDYPNLCQQLGLRE
LITRYEALPIAKQGNLLQLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGATNQGKEISQDELAN
LVKVGDEERQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYEAQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNSDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKVAVRPSAILQNQFAFQSQE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMRRATIHQIESLAKTNGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=167865 AL543_RS07645 WP_061051580.1 198072..199760(+) (pilB) [Vibrio mimicus strain FDAARGOS_112]
ATGCTCACCAATCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCTCAACGCAAGAACAAGCGGTGGTCGAACAAGT
CAGCGCTTCTGGTACTTCAGTGCCGGAAGCGCTGCTAGAATTAAGTATTTTCCACGCTCAAGAACTCACTGAACAAATCA
GCCATATTTTCGGCTTACCGGAAACCGATTTAAGCCACTACGATTACCCTAATCTCTGCCAACAACTTGGTTTACGTGAG
CTGATTACCCGTTATGAAGCGCTCCCCATTGCCAAACAAGGCAATCTACTTCAACTCGCCGTCTCTGACCCCACTTTACT
GCAAGCTGAAGAAGAGTTTCGCTTTGCCACGGGCTTACAAGTGGAGTTGGCACTCGCAGATCATCGTGCACTACAAGCAG
CCATTCGTCGTTTGTATGGCCGATCCATTCAGGGTGCTACCAACCAAGGTAAAGAGATCAGCCAAGATGAATTAGCCAAT
CTCGTCAAAGTCGGGGATGAAGAACGTCAGTCAATTGAAGACCTAAGCCAAGATGATTCTCCGGTCAGCCGCTTTATCAA
CCAAGTATTGCTGGATGCGGTACGTAAAGGCGCATCCGACATTCATTTTGAGCCTTATGAAGCGCAATACCGCATTCGCC
TGCGCTGCGATGGTATTTTGGTGGAAACCCAACAACCTGCTAGCCATTTAAGCCGTCGCTTAGCCGCCCGAATTAAGATT
CTCTCTAAACTGGACATTGCTGAACGCCGTTTGCCACAAGATGGCCGAATCAAGTTGCGTCTTAATAGTGATACCGCGAT
TGATATGCGCGTATCAACTTTACCCACCTTGTGGGGTGAAAAAATTGTATTGCGCCTGCTCGATAGCAGCGCTGCAAATC
TGGATATTGATAAGCTCGGTTACAACCCGCAGCAGAAGCAACTTTATCTCAATGCACTCAAAAGACCGCAAGGAATGATA
TTGATGACAGGCCCGACCGGTAGCGGCAAAACCGTCTCACTCTATACTGGACTGCGCATTCTCAATACCTCGCAAATTAA
TATTTCCACCGCCGAAGATCCGGTTGAAATTAACCTCTCCGGCATTAACCAAGTCCAGGTGCAACCGAAAATCGGTTTTG
GCTTTGCCGAAGCTCTACGCTCATTTCTACGCCAAGACCCCGACGTGGTGATGGTGGGCGAAATTCGCGATTTGGAAACC
GCTGAAATTGCGGTCAAGGCGGCGCAAACGGGTCACTTGGTGCTTTCAACTTTGCATACCAATTCAGCAGCGGAAACTGT
CATTCGCTTAGCCAACATGGGGGTTGAACCCTTTAACCTCGCTTCATCACTTAGTTTGATTATCGCCCAGCGCCTGGCTC
GCCGTTTATGTAAACACTGCAAAGTGGCGGTACGTCCGTCAGCCATACTGCAAAATCAGTTTGCCTTTCAATCTCAAGAA
ATTTTGTATGAAGCGAATGCGGCAGGCTGCAACGAATGCACAGGTGGTTATTCAGGGCGAGTCGGGATCTATGAGGTCAT
GGCATTTAATACTGAACTGGCAGAGGCCATTATGCGCCGTGCCACCATTCACCAGATTGAAAGTTTAGCCAAAACTAATG
GCATGCAAACCCTGCAAGAGTCCGGCCTTGAAAAGCTGCGTGAAGGCATCACCAGTTTTGCCGAACTACAACGTGTGCTT
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

95.907

100

0.959
  pilB Vibrio parahaemolyticus RIMD 2210633

73.132

100

0.731
  pilB Vibrio campbellii strain DS40M4

72.954

100

0.73
  pilB Legionella pneumophila strain ERS1305867

49.369

98.754

0.488
  pilB Acinetobacter baylyi ADP1

50.579

92.171

0.466
  pilB Acinetobacter baumannii D1279779

50.289

92.349

0.464
  pilF Neisseria gonorrhoeae MS11

45.907

100

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.271

100

0.399
  pilF Thermus thermophilus HB27

39.926

96.263

0.384
  pilB Haemophilus influenzae 86-028NP

39.205

93.95

0.368
  pilB Haemophilus influenzae Rd KW20

38.931

93.238

0.363

Multiple sequence alignment