Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AL543_RS07655 Genome accession   NZ_CP014043
Coordinates   201088..201963 (+) Length   291 a.a.
NCBI ID   WP_061051582.1    Uniprot ID   -
Organism   Vibrio mimicus strain FDAARGOS_112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196088..206963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL543_RS07635 (AL543_06905) nadC 196467..197357 (+) 891 WP_061051578.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL543_RS07640 (AL543_06910) pilA 197602..198072 (+) 471 WP_061051579.1 pilin Machinery gene
  AL543_RS07645 (AL543_06915) pilB 198072..199760 (+) 1689 WP_061051580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL543_RS07650 (AL543_06920) pilC 199799..201025 (+) 1227 WP_061051581.1 type II secretion system F family protein Machinery gene
  AL543_RS07655 (AL543_06925) pilD 201088..201963 (+) 876 WP_061051582.1 prepilin peptidase Machinery gene
  AL543_RS07660 (AL543_06930) coaE 201960..202568 (+) 609 WP_061051583.1 dephospho-CoA kinase -
  AL543_RS07665 (AL543_06935) zapD 202600..203340 (+) 741 WP_061051584.1 cell division protein ZapD -
  AL543_RS07670 (AL543_06940) yacG 203591..203788 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  AL543_RS07675 (AL543_06945) parC 203837..206122 (-) 2286 WP_061051585.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32413.61 Da        Isoelectric Point: 6.6889

>NTDB_id=167867 AL543_RS07655 WP_061051582.1 201088..201963(+) (pilD) [Vibrio mimicus strain FDAARGOS_112]
MELFFYYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRADCAASFPEYGISPPEGQLTLSLPRSTCPHCQTPIRVI
DNIPVVSWLALRGKCSHCKSPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTMLLPDQLTLP
LLWSGIALALFELSPVSLTDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGL
LFGLIQLRLQKQGIDKAFPFGPYLAIAGWIALLWGNTVIDWYLTSWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=167867 AL543_RS07655 WP_061051582.1 201088..201963(+) (pilD) [Vibrio mimicus strain FDAARGOS_112]
ATGGAACTGTTTTTCTACTACCCTTGGCTGTTTCCCGTTTTGGCTACCTTATTTGGGCTTATCGTCGGCAGCTTTCTTAA
TGTGGTGATTTATCGCTTACCGAAAATCATGGAGCGTGAGTGGCGTGCCGACTGTGCGGCCAGCTTTCCGGAATACGGAA
TAAGCCCACCAGAAGGGCAACTTACGCTCAGTTTGCCACGCTCAACCTGCCCACATTGCCAAACACCCATTCGAGTGATT
GATAATATTCCGGTAGTCAGTTGGCTTGCACTGCGAGGAAAATGCTCACACTGCAAATCACCAATCAGTGCCCGTTATCC
CTTAATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCATTCGGTGTATTTGCCGTGGCGTTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCAACTTTCATCGACTTCGATACTATGCTGTTGCCTGACCAGCTCACATTGCCT
CTGCTGTGGAGTGGCATTGCGTTAGCCTTGTTTGAACTGTCCCCGGTGTCGCTTACCGATGCAGTGATTGGTGCAATGGC
GGGTTATCTTTCACTGTGGTCGATTTACTGGTTATTTAAATTACTGACCGGCAAAGAAGGCATGGGTTATGGGGATTTCA
AATTGCTCGCCGCCCTTGGAGCTTGGCTGGGTTGGCAACAGTTGCCAGTCATCGTACTGCTCTCGTCTGTGGTTGGCCTT
CTCTTCGGTTTAATTCAGTTACGCCTACAGAAACAAGGCATAGATAAAGCTTTCCCATTTGGCCCATATTTAGCCATTGC
TGGGTGGATTGCCTTACTGTGGGGCAATACAGTGATTGACTGGTACTTAACCTCTTGGGTAGGGCAACCATTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

91.409

100

0.914
  pilD Vibrio campbellii strain DS40M4

76.042

98.969

0.753
  pilD Acinetobacter baumannii D1279779

54.044

93.471

0.505
  pilD Acinetobacter nosocomialis M2

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

46.35

94.158

0.436

Multiple sequence alignment