Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL538_RS08385 Genome accession   NZ_CP014038
Coordinates   1643322..1645073 (-) Length   583 a.a.
NCBI ID   WP_005451505.1    Uniprot ID   A0A9X3RXD0
Organism   Vibrio harveyi strain FDAARGOS_107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1638322..1650073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL538_RS08365 (AL538_07745) - 1639017..1639856 (-) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AL538_RS29375 - 1639926..1640507 (-) 582 Protein_1503 SPOR domain-containing protein -
  AL538_RS29380 - 1640585..1641428 (-) 844 Protein_1504 ATP-binding protein -
  AL538_RS08375 (AL538_07755) aroB 1641482..1642582 (-) 1101 WP_038898114.1 3-dehydroquinate synthase -
  AL538_RS08380 (AL538_07760) aroK 1642614..1643132 (-) 519 WP_005451507.1 shikimate kinase AroK -
  AL538_RS08385 (AL538_07765) pilQ 1643322..1645073 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  AL538_RS08390 (AL538_07770) pilP 1645108..1645623 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  AL538_RS08395 (AL538_07775) pilO 1645613..1646206 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL538_RS08400 (AL538_07780) pilN 1646199..1646774 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  AL538_RS08405 (AL538_07785) pilM 1646758..1647774 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63768.79 Da        Isoelectric Point: 4.5053

>NTDB_id=167782 AL538_RS08385 WP_005451505.1 1643322..1645073(-) (pilQ) [Vibrio harveyi strain FDAARGOS_107]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGEDGGGSVGIDDFLNVNLAATSPN
ATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTP
QITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALF
RRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=167782 AL538_RS08385 WP_005451505.1 1643322..1645073(-) (pilQ) [Vibrio harveyi strain FDAARGOS_107]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGGCGTTATC
TAGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATTCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGTGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGCCCGAAT
GCAACCAGTATTGCTTTCCAAGTTGCGAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCAACAAGA
ATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTACTGAAA
TTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTGACACCG
CAGATCACGCCTGATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTAC
CGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTG
GTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTC
CGCCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTGACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(423-581)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

97.084

100

0.971
  pilQ Vibrio cholerae strain A1552

77.329

97.599

0.755
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.329

97.599

0.755
  pilQ Pseudomonas aeruginosa PAK

40.138

99.142

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.34

0.389
  pilQ Legionella pneumophila strain Lp02

41.273

94.34

0.389
  pilQ Acinetobacter baumannii D1279779

36.833

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.833

100

0.379
  comQ Acinetobacter baylyi ADP1

36.986

100

0.37

Multiple sequence alignment