Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AL538_RS08405 Genome accession   NZ_CP014038
Coordinates   1646758..1647774 (-) Length   338 a.a.
NCBI ID   WP_026000383.1    Uniprot ID   A0A3A1Q0R6
Organism   Vibrio harveyi strain FDAARGOS_107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1641758..1652774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL538_RS08380 (AL538_07760) aroK 1642614..1643132 (-) 519 WP_005451507.1 shikimate kinase AroK -
  AL538_RS08385 (AL538_07765) pilQ 1643322..1645073 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  AL538_RS08390 (AL538_07770) pilP 1645108..1645623 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  AL538_RS08395 (AL538_07775) pilO 1645613..1646206 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL538_RS08400 (AL538_07780) pilN 1646199..1646774 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  AL538_RS08405 (AL538_07785) pilM 1646758..1647774 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene
  AL538_RS08410 (AL538_07790) - 1647953..1650523 (+) 2571 WP_050935720.1 penicillin-binding protein 1A -
  AL538_RS08415 (AL538_07795) oxyR 1650590..1651498 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  AL538_RS08420 (AL538_07800) - 1651673..1652401 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38066.43 Da        Isoelectric Point: 6.4206

>NTDB_id=167786 AL538_RS08405 WP_026000383.1 1646758..1647774(-) (pilM) [Vibrio harveyi strain FDAARGOS_107]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDSQVATEQFN
RQIVDRVKRQIQLYTSINGNQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=167786 AL538_RS08405 WP_026000383.1 1646758..1647774(-) (pilM) [Vibrio harveyi strain FDAARGOS_107]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAACAAGACCCTCTTGCTGAAGACTCGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAATCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A1Q0R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment