Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   RN86_RS04150 Genome accession   NZ_CP012648
Coordinates   842221..843159 (+) Length   312 a.a.
NCBI ID   WP_046164736.1    Uniprot ID   -
Organism   Streptococcus gordonii strain KCOM 1506 (= ChDC B679)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 837221..848159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN86_RS04140 (RN86_04135) rpoC 837853..841500 (+) 3648 WP_045634165.1 DNA-directed RNA polymerase subunit beta' -
  RN86_RS04145 (RN86_04140) - 841751..842119 (+) 369 WP_046164737.1 DUF1033 family protein -
  RN86_RS04150 (RN86_04145) comYA 842221..843159 (+) 939 WP_046164736.1 competence type IV pilus ATPase ComGA Machinery gene
  RN86_RS04155 (RN86_04150) comYB 843092..844126 (+) 1035 WP_046164735.1 competence type IV pilus assembly protein ComGB Machinery gene
  RN86_RS04160 (RN86_04155) comYC 844123..844440 (+) 318 WP_012130940.1 competence type IV pilus major pilin ComGC Machinery gene
  RN86_RS04165 (RN86_04160) comYD 844400..844828 (+) 429 WP_069097080.1 competence type IV pilus minor pilin ComGD Machinery gene
  RN86_RS04170 (RN86_04165) comGE/cglE 844800..845093 (+) 294 WP_046164733.1 competence type IV pilus minor pilin ComGE Machinery gene
  RN86_RS04175 (RN86_04170) comGF/cglF 845077..845514 (+) 438 WP_053794327.1 competence type IV pilus minor pilin ComGF Machinery gene
  RN86_RS04180 (RN86_04175) comGG 845495..845926 (+) 432 WP_253276489.1 competence type IV pilus minor pilin ComGG -
  RN86_RS04185 (RN86_04180) comYH 845957..846910 (+) 954 WP_053794328.1 class I SAM-dependent methyltransferase Machinery gene
  RN86_RS04190 (RN86_04185) - 846960..848150 (+) 1191 WP_045634156.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35828.81 Da        Isoelectric Point: 5.4496

>NTDB_id=156070 RN86_RS04150 WP_046164736.1 842221..843159(+) (comYA) [Streptococcus gordonii strain KCOM 1506 (= ChDC B679)]
MVQKIAQAIVRQAKEECAQDIYFVPKDDCYELYMRIGDERRFIQTYDFDQLAAVISHFKFLAGMNVGEKRRSQLGSCDYR
YDDKETSIRLSTVGDYRGYESLVIRLLHDEETELKFWFTHFPEFREKFKDRGLYLFSGPVGSGKTTLMHQLAQLKFKGQQ
VMSIEDPVEIKQEDMLQLQLNETIGLTYESLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIPGVY
ERLLELGVSEEELKIVLQGICYQRLIGGGGVIDFASNNYQEHEPTVWNQQIDQLLAAGHIHPEQAEAEKIRN

Nucleotide


Download         Length: 939 bp        

>NTDB_id=156070 RN86_RS04150 WP_046164736.1 842221..843159(+) (comYA) [Streptococcus gordonii strain KCOM 1506 (= ChDC B679)]
ATGGTTCAAAAAATTGCCCAGGCGATTGTCCGGCAGGCTAAGGAAGAGTGTGCTCAGGATATTTATTTTGTACCCAAGGA
TGATTGTTATGAACTTTATATGCGGATTGGTGATGAACGACGCTTTATACAGACTTATGATTTTGATCAATTAGCCGCTG
TGATTAGTCATTTTAAATTTCTAGCAGGGATGAATGTAGGAGAAAAACGTCGCAGTCAGCTGGGATCCTGTGATTATCGT
TATGATGATAAGGAAACGTCAATTCGTCTGTCAACTGTAGGTGATTATCGTGGATATGAAAGTTTGGTTATTCGACTTCT
GCATGACGAAGAGACAGAATTGAAGTTTTGGTTTACTCATTTTCCTGAGTTTAGAGAGAAGTTTAAGGATCGAGGACTGT
ATCTTTTTTCGGGACCAGTAGGGAGTGGTAAGACCACTCTCATGCATCAATTAGCTCAGCTAAAATTTAAGGGTCAGCAG
GTTATGTCCATTGAGGATCCTGTTGAAATCAAGCAAGAGGATATGCTTCAGCTACAGCTGAATGAAACGATTGGTCTGAC
CTATGAAAGTCTAATTAAGCTTTCCCTGCGTCACCGTCCGGACTTGCTCATCATTGGAGAAATTCGGGATAGTGAAACCG
CTAGAGCAGTGGTTCGAGCTAGCTTGACGGGAGCTACAGTTTTTTCAACGATTCATGCTAAAAGCATTCCTGGTGTTTAC
GAACGCTTATTGGAATTAGGTGTTAGCGAGGAGGAGTTGAAAATTGTTTTACAGGGTATTTGCTATCAACGCTTGATTGG
GGGAGGAGGTGTGATAGACTTTGCCAGCAACAACTACCAAGAACACGAGCCGACCGTCTGGAATCAGCAGATTGATCAGC
TTCTTGCAGCAGGACATATCCATCCTGAGCAAGCAGAGGCAGAAAAAATTAGGAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

99.359

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

78.065

99.359

0.776
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

77.742

99.359

0.772
  comGA/cglA/cilD Streptococcus pneumoniae D39

77.742

99.359

0.772
  comGA/cglA/cilD Streptococcus pneumoniae R6

77.742

99.359

0.772
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

77.742

99.359

0.772
  comYA Streptococcus mutans UA159

67.093

100

0.673
  comYA Streptococcus mutans UA140

67.093

100

0.673
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

65.064

100

0.651
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

100

0.548

Multiple sequence alignment