Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AKN94_RS09420 Genome accession   NZ_CP012359
Coordinates   1965367..1967040 (+) Length   557 a.a.
NCBI ID   WP_053111290.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain C6819     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1960367..1972040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN94_RS09400 (AKN94_09395) pilM 1962534..1963607 (+) 1074 WP_064496080.1 type IV pilus assembly protein PilM -
  AKN94_RS09405 (AKN94_09400) - 1963594..1964157 (+) 564 WP_053104864.1 PilN domain-containing protein -
  AKN94_RS10940 (AKN94_09405) - 1964169..1964780 (+) 612 WP_064496081.1 type 4a pilus biogenesis protein PilO -
  AKN94_RS09415 (AKN94_09410) - 1964777..1965307 (+) 531 WP_053111289.1 pilus assembly protein PilP -
  AKN94_RS09420 (AKN94_09415) pilQ 1965367..1967040 (+) 1674 WP_053111290.1 type IV pilus secretin PilQ Machinery gene
  AKN94_RS09425 (AKN94_09420) aroK 1967046..1967570 (+) 525 WP_080995523.1 shikimate kinase AroK -
  AKN94_RS09430 (AKN94_09425) aroB 1967621..1968733 (+) 1113 WP_053111322.1 3-dehydroquinate synthase -
  AKN94_RS09435 (AKN94_09430) - 1968737..1969912 (+) 1176 WP_053100533.1 SPOR domain-containing protein -
  AKN94_RS09440 (AKN94_09435) - 1970019..1970990 (+) 972 WP_053100532.1 phosphate ABC transporter substrate-binding protein PstS family protein -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 61987.12 Da        Isoelectric Point: 6.0665

>NTDB_id=153759 AKN94_RS09420 WP_053111290.1 1965367..1967040(+) (pilQ) [Thiopseudomonas alkaliphila strain C6819]
MVANFFQRFIMLLVIILFDAMLALQLAASELTTYSKLVSNTQVENLYFKRGGQGEGNVILTLTEPDVRFEVKEESDKIHL
VLLNTSLSKQINTHLKTKEFATPVQLISMSQSGKDVSVIIELIDSYKYAVHQVGRQITVSVNVLPYKALEEHHSKNIRYV
GEKISLNFQDVNLRAVLQVIADFTQLNLVVNDTVQGNITLQLQNVPWDQALDVILKTKGLAQRKEGNVLLIAPSEELAAR
ERQELEIQRQAEELAPLRRELIQINYAKATDIAALFRLASGEQTEERGIITVDERTNAIIAYQTQERLAELSKIVSQLDT
PVRQVMIEARIVEASVDYEKELGVRWGGERRGSSTHYNINNAFVDLGANNASSGVGIGFLSGKTRLDLELSAMEKTGNGE
VVSQPKIITSDKETAKIMKGSEVPYQEASSSGATSTSFKQAALSLEVTPYITPDNRILMEVRVTKDAPDFQKAINGVPPI
DKNELSAKVLVVDGETIVIGGVFQKVQKESLEKVPVLGDIPGLKGLFRRKMRRDDKTELLVFITPRIMNNQSISLNR

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=153759 AKN94_RS09420 WP_053111290.1 1965367..1967040(+) (pilQ) [Thiopseudomonas alkaliphila strain C6819]
ATGGTAGCTAATTTTTTTCAGCGATTTATTATGCTGTTAGTAATTATACTTTTCGATGCAATGTTGGCTCTACAATTGGC
GGCTTCAGAGTTAACAACCTATTCTAAATTAGTCAGTAATACCCAGGTTGAAAATTTATATTTTAAGCGCGGTGGACAAG
GAGAGGGTAATGTCATTTTGACCTTAACAGAACCTGATGTCCGATTTGAAGTAAAAGAAGAGTCTGACAAGATTCACTTG
GTTTTATTAAATACTAGCTTGTCCAAACAAATAAATACTCACTTAAAAACTAAAGAGTTTGCAACACCTGTTCAACTGAT
TAGTATGAGTCAATCCGGAAAAGATGTATCAGTTATAATTGAGTTAATAGATAGCTATAAGTACGCAGTGCATCAAGTAG
GTAGGCAAATAACTGTGAGTGTAAACGTATTACCCTATAAAGCACTAGAGGAACATCATTCGAAAAATATACGTTATGTG
GGCGAAAAGATATCATTGAATTTTCAAGATGTTAATCTGAGAGCAGTACTACAAGTTATTGCTGACTTTACTCAGCTTAA
TCTGGTGGTTAATGATACAGTGCAAGGCAATATTACATTGCAATTACAAAATGTGCCTTGGGATCAAGCGCTGGATGTAA
TATTAAAAACTAAAGGGCTTGCTCAGCGTAAAGAGGGAAACGTGTTGTTGATTGCGCCCTCTGAGGAACTGGCAGCACGT
GAGCGTCAGGAGTTGGAAATACAGCGTCAAGCTGAAGAACTAGCCCCATTACGTCGTGAGTTAATTCAAATTAACTATGC
TAAAGCTACCGATATTGCCGCGTTATTCCGGTTGGCCTCAGGGGAGCAAACAGAAGAACGCGGAATTATTACCGTGGATG
AGCGGACCAATGCAATTATTGCTTACCAAACACAAGAGCGTTTAGCAGAGCTTAGTAAAATTGTCTCTCAGCTAGATACA
CCAGTACGTCAAGTAATGATTGAGGCGCGAATTGTTGAAGCTAGCGTGGACTATGAAAAAGAACTTGGAGTTAGATGGGG
AGGAGAGCGTAGAGGGAGCAGTACGCATTATAATATTAACAATGCTTTTGTAGACTTGGGTGCCAATAATGCGAGCTCTG
GAGTAGGCATCGGTTTTCTCAGTGGTAAAACTCGATTAGATCTAGAATTGTCAGCAATGGAGAAAACTGGCAATGGGGAA
GTTGTTTCGCAGCCCAAAATTATTACATCAGATAAAGAAACAGCGAAAATCATGAAGGGCTCGGAAGTGCCTTACCAAGA
GGCCAGCTCCAGTGGCGCTACCAGTACTTCATTTAAACAGGCAGCTCTATCGTTAGAGGTTACACCCTACATTACGCCTG
ATAATCGTATTTTGATGGAAGTAAGAGTGACTAAAGACGCGCCAGATTTTCAAAAGGCTATTAATGGTGTTCCGCCGATT
GATAAGAATGAGTTAAGTGCTAAGGTGTTAGTGGTGGATGGTGAAACTATCGTAATTGGTGGCGTGTTTCAAAAAGTACA
AAAAGAGTCCTTAGAAAAAGTGCCAGTGTTGGGAGATATCCCAGGCCTTAAAGGATTGTTCCGCCGTAAGATGAGGCGAG
ATGATAAAACTGAATTATTAGTTTTCATTACGCCAAGAATCATGAATAATCAAAGTATTTCCTTGAATCGTTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(186-233)

Bacterial type II/III secretion system short domain (Secretin_N)

(261-324)

Bacterial type II and III secretion system protein (Secretin)

(392-548)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.239

99.102

0.587
  pilQ Acinetobacter baumannii D1279779

39.826

100

0.411
  pilQ Acinetobacter baumannii strain A118

39.826

100

0.411
  comQ Acinetobacter baylyi ADP1

39.75

100

0.4
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.42

100

0.384
  pilQ Vibrio cholerae strain A1552

38.42

100

0.384
  pilQ Legionella pneumophila strain ERS1305867

40.655

93.178

0.379
  pilQ Vibrio campbellii strain DS40M4

38.251

98.564

0.377
  pilQ Legionella pneumophila strain Lp02

40.462

93.178

0.377

Multiple sequence alignment