Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   ECTOBSL9_RS16335 Genome accession   NZ_CP011994
Coordinates   559188..559604 (+) Length   138 a.a.
NCBI ID   WP_082829697.1    Uniprot ID   A0A161HEN5
Organism   Ectothiorhodospira sp. BSL-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 549097..579300 559188..559604 within 0


Gene organization within MGE regions


Location: 549097..579300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECTOBSL9_RS02745 (ECTOBSL9_0551) - 549097..549480 (-) 384 WP_063463773.1 type IV pilin protein -
  ECTOBSL9_RS02750 (ECTOBSL9_0552) - 549552..553115 (-) 3564 WP_063463774.1 pilus assembly protein -
  ECTOBSL9_RS02755 (ECTOBSL9_0553) - 553183..553677 (-) 495 WP_063463775.1 PilX N-terminal domain-containing pilus assembly protein -
  ECTOBSL9_RS02760 (ECTOBSL9_0554) - 553688..554746 (-) 1059 WP_082829696.1 PilW family protein -
  ECTOBSL9_RS02765 (ECTOBSL9_0555) pilV 554774..555169 (-) 396 WP_063463777.1 type IV pilus modification protein PilV -
  ECTOBSL9_RS02770 (ECTOBSL9_0556) - 555169..555651 (-) 483 WP_063463778.1 GspH/FimT family pseudopilin -
  ECTOBSL9_RS02775 (ECTOBSL9_0557) - 555944..556408 (-) 465 WP_063463779.1 tetratricopeptide repeat protein -
  ECTOBSL9_RS02780 - 556413..557624 (-) 1212 WP_063463780.1 PilX N-terminal domain-containing pilus assembly protein -
  ECTOBSL9_RS02785 (ECTOBSL9_0559) - 557663..558226 (-) 564 WP_063463781.1 PilW family protein -
  ECTOBSL9_RS02790 (ECTOBSL9_0560) pilV 558226..558717 (-) 492 WP_063463782.1 type IV pilus modification protein PilV -
  ECTOBSL9_RS16335 (ECTOBSL9_0561) pilA2 559188..559604 (+) 417 WP_082829697.1 pilin Machinery gene
  ECTOBSL9_RS02795 (ECTOBSL9_0562) - 559963..560673 (-) 711 WP_063463783.1 hypothetical protein -
  ECTOBSL9_RS02800 (ECTOBSL9_0563) - 560972..561925 (-) 954 WP_063463784.1 transposase -
  ECTOBSL9_RS17375 - 562065..562655 (+) 591 WP_240481037.1 BrnT family toxin -
  ECTOBSL9_RS02810 (ECTOBSL9_0565) - 562739..563038 (+) 300 WP_063463785.1 hypothetical protein -
  ECTOBSL9_RS02815 (ECTOBSL9_0566) - 563993..564946 (-) 954 WP_063463786.1 transposase -
  ECTOBSL9_RS02820 (ECTOBSL9_0567) - 565086..565367 (+) 282 WP_063463787.1 BrnT family toxin -
  ECTOBSL9_RS02825 (ECTOBSL9_0568) - 565342..565677 (+) 336 WP_063463788.1 DNA-binding transcriptional regulator -
  ECTOBSL9_RS02830 (ECTOBSL9_0569) - 565872..566831 (-) 960 WP_063463789.1 Rpn family recombination-promoting nuclease/putative transposase -
  ECTOBSL9_RS17385 - 567608..568198 (+) 591 WP_240481037.1 BrnT family toxin -
  ECTOBSL9_RS02845 (ECTOBSL9_0572) - 568282..568581 (+) 300 WP_063463785.1 hypothetical protein -
  ECTOBSL9_RS02850 (ECTOBSL9_0573) - 568899..569096 (+) 198 WP_063463791.1 hypothetical protein -
  ECTOBSL9_RS02855 (ECTOBSL9_0574) - 569093..569950 (+) 858 WP_082829698.1 Rpn family recombination-promoting nuclease/putative transposase -
  ECTOBSL9_RS02860 (ECTOBSL9_0575) - 570245..571198 (-) 954 WP_063463792.1 transposase -
  ECTOBSL9_RS02865 (ECTOBSL9_0576) - 571245..571634 (-) 390 WP_063463793.1 type II toxin-antitoxin system VapC family toxin -
  ECTOBSL9_RS02870 (ECTOBSL9_0577) - 571634..571873 (-) 240 WP_063463794.1 hypothetical protein -
  ECTOBSL9_RS17790 (ECTOBSL9_0578) - 572006..572287 (-) 282 WP_063463795.1 hypothetical protein -
  ECTOBSL9_RS02880 (ECTOBSL9_0579) - 572979..573236 (+) 258 WP_063463796.1 type II toxin-antitoxin system HicA family toxin -
  ECTOBSL9_RS02885 (ECTOBSL9_0580) - 573233..573586 (+) 354 WP_063463797.1 type II toxin-antitoxin system HicB family antitoxin -
  ECTOBSL9_RS02890 (ECTOBSL9_0581) - 573707..574330 (+) 624 WP_063465969.1 IS607 family transposase -
  ECTOBSL9_RS02895 (ECTOBSL9_0582) - 574327..575520 (+) 1194 WP_063463798.1 RNA-guided endonuclease TnpB family protein -
  ECTOBSL9_RS02900 (ECTOBSL9_0583) - 575980..576279 (+) 300 WP_063463799.1 BrnT family toxin -
  ECTOBSL9_RS02905 (ECTOBSL9_0584) - 576245..576580 (+) 336 WP_082829699.1 BrnA antitoxin family protein -
  ECTOBSL9_RS02910 (ECTOBSL9_0585) - 576938..577135 (+) 198 WP_063463791.1 hypothetical protein -
  ECTOBSL9_RS02915 (ECTOBSL9_0586) - 577132..578022 (+) 891 WP_063463801.1 Rpn family recombination-promoting nuclease/putative transposase -
  ECTOBSL9_RS17395 (ECTOBSL9_0587) - 578182..578556 (-) 375 Protein_577 hypothetical protein -
  ECTOBSL9_RS02925 (ECTOBSL9_0588) - 578719..579009 (+) 291 WP_063463803.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  ECTOBSL9_RS02930 (ECTOBSL9_0589) - 579013..579300 (+) 288 WP_063463804.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence


Protein


Download         Length: 138 a.a.        Molecular weight: 14760.62 Da        Isoelectric Point: 4.1958

>NTDB_id=149979 ECTOBSL9_RS16335 WP_082829697.1 559188..559604(+) (pilA2) [Ectothiorhodospira sp. BSL-9]
MQRQQGFTLIELMIVVAIIGILAAIALPAYQNYTVRAQVSEIILAASAGRTDISEYVSVEGELPPATYTVETQQSTKVAS
STWDGTDVIVTAATGIHDDVDGETINLRPNVTGNTANWTCGGSIPARFRPASCRDFDI

Nucleotide


Download         Length: 417 bp        

>NTDB_id=149979 ECTOBSL9_RS16335 WP_082829697.1 559188..559604(+) (pilA2) [Ectothiorhodospira sp. BSL-9]
ATGCAGAGACAACAAGGTTTTACTTTGATTGAACTGATGATCGTGGTGGCGATTATTGGGATTTTGGCGGCGATTGCGCT
GCCGGCTTATCAAAACTATACGGTTCGCGCTCAAGTCTCCGAGATCATTCTTGCGGCTTCCGCAGGACGAACTGATATTT
CCGAATATGTATCAGTGGAAGGCGAACTTCCACCGGCCACCTACACAGTGGAAACACAGCAATCGACAAAGGTGGCAAGC
TCAACATGGGATGGTACTGATGTAATTGTTACCGCGGCTACTGGCATACATGACGACGTAGACGGGGAAACAATTAACTT
GCGTCCTAACGTAACGGGCAATACTGCGAACTGGACCTGTGGTGGCTCAATCCCCGCTAGATTCCGTCCAGCGAGCTGTA
GAGACTTCGATATCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A161HEN5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

47.518

100

0.486

  pilA2 Legionella pneumophila strain ERS1305867

47.143

100

0.478

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.326

100

0.471

  pilA/pilA1 Eikenella corrodens VA1

40.789

100

0.449

  pilE Neisseria gonorrhoeae MS11

37.654

100

0.442

  comP Acinetobacter baylyi ADP1

39.597

100

0.428

  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.568

100

0.399

  pilE Neisseria gonorrhoeae strain FA1090

34.839

100

0.391

  pilA Acinetobacter baumannii strain A118

36.879

100

0.377

  pilA Pseudomonas aeruginosa PAK

38.462

94.203

0.362

  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.496

99.275

0.362


Multiple sequence alignment