Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   UC35_RS03715 Genome accession   NZ_CP010951
Coordinates   762211..763428 (+) Length   405 a.a.
NCBI ID   WP_061496306.1    Uniprot ID   A0A127JQF6
Organism   Ramlibacter tataouinensis strain 5-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 757211..768428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC35_RS03700 (UC35_03695) - 757285..758895 (+) 1611 WP_158513854.1 alkaline phosphatase family protein -
  UC35_RS03705 (UC35_03700) - 758892..760292 (+) 1401 WP_061496302.1 glycosyltransferase -
  UC35_RS03710 (UC35_03705) pilB 760437..762176 (+) 1740 WP_061496304.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UC35_RS03715 (UC35_03710) pilC 762211..763428 (+) 1218 WP_061496306.1 type II secretion system F family protein Machinery gene
  UC35_RS03720 (UC35_03715) pilD 763428..764270 (+) 843 WP_061496308.1 prepilin peptidase Machinery gene
  UC35_RS03725 (UC35_03720) coaE 764273..764878 (+) 606 WP_061496310.1 dephospho-CoA kinase -
  UC35_RS03730 (UC35_03725) zapD 764886..765641 (+) 756 WP_061496312.1 cell division protein ZapD -
  UC35_RS03735 (UC35_03730) - 765641..765853 (+) 213 WP_061496314.1 DNA gyrase inhibitor YacG -
  UC35_RS03740 (UC35_03735) - 765854..766321 (-) 468 WP_061496316.1 NUDIX domain-containing protein -
  UC35_RS03745 (UC35_03740) - 766318..767184 (-) 867 WP_061496319.1 ATP-binding protein -
  UC35_RS03750 (UC35_03745) argJ 767181..768407 (-) 1227 WP_061496320.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44303.98 Da        Isoelectric Point: 9.3345

>NTDB_id=140847 UC35_RS03715 WP_061496306.1 762211..763428(+) (pilC) [Ramlibacter tataouinensis strain 5-10]
MATAAATKVTEFVFEWEGRDRNGKQVRGETRAAGENQVQAALRRQGVTPTKIKKRRMRSGQKIKPKDIAIFTRQLATMMK
AGVPLLQAFDIVGRGNSNASVMKLLNDIRTDVETGTSLSSAFRKYPLYFDNLYCNLVEAGEAAGILETLLDRLAVYMEKT
EAIKSKIKSALMYPISVLVVAFVVVSVIMIFVIPAFKEVFTSFGADLPAPTLFVIALSEFFVKFWWLIFGSVGGGVYFFF
QAWKRSEKVQVFMDRLMLKLPVFGDLVYKSVIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYVGATQKIQTEVSTGT
SLTAAMTNANVFPTMVLQMCAIGEESGALDHMLSKAADFYEAEVDDMVEGLSSLMEPIIIVFLGGLIGGIVVSMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=140847 UC35_RS03715 WP_061496306.1 762211..763428(+) (pilC) [Ramlibacter tataouinensis strain 5-10]
ATGGCTACCGCAGCTGCAACCAAAGTGACGGAATTCGTCTTCGAGTGGGAAGGCCGCGACCGCAACGGCAAGCAGGTCCG
TGGCGAGACCCGCGCCGCGGGCGAGAACCAGGTGCAGGCCGCCCTGCGGCGCCAGGGCGTCACGCCGACCAAGATCAAGA
AGCGCCGCATGCGCTCCGGCCAGAAGATCAAGCCGAAGGACATCGCGATCTTCACCCGCCAGCTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTTCAAGCCTTTGACATCGTGGGCCGGGGCAACTCCAACGCCAGCGTGATGAAGCTGCTCAACGA
CATCCGCACGGACGTCGAGACCGGCACCTCCCTGTCTTCGGCGTTCCGCAAGTACCCGCTGTACTTCGACAACCTGTACT
GCAACCTGGTCGAGGCCGGCGAAGCGGCCGGTATTCTGGAAACCCTTCTGGACCGGCTCGCGGTCTACATGGAAAAGACC
GAGGCGATCAAGAGCAAGATCAAGTCGGCCCTGATGTACCCCATCTCGGTGCTGGTGGTTGCCTTCGTGGTGGTGTCGGT
GATCATGATCTTCGTGATTCCCGCCTTCAAAGAGGTGTTCACCTCGTTCGGTGCGGACCTGCCCGCGCCCACCCTGTTCG
TGATCGCCCTGAGCGAGTTCTTCGTCAAGTTTTGGTGGCTGATCTTCGGCAGCGTGGGCGGGGGGGTGTACTTCTTCTTC
CAGGCCTGGAAGCGAAGCGAGAAGGTCCAGGTGTTCATGGACCGCCTCATGCTCAAGCTGCCGGTGTTCGGCGACCTGGT
GTACAAGTCGGTGATCGCGCGCTGGACCCGCACGCTGGCCACCATGTTCGCCGCCGGCGTGCCGCTGGTGGAAGCGCTCG
ACTCGGTCGGCGGCGCGTCGGGTAACTCGGTCTACGTCGGCGCCACGCAAAAAATCCAGACGGAAGTCTCCACCGGCACC
AGCCTGACGGCGGCCATGACCAACGCCAACGTGTTCCCCACCATGGTGCTGCAGATGTGCGCCATCGGCGAGGAATCCGG
CGCGCTCGACCACATGCTGAGCAAGGCCGCCGACTTTTACGAAGCGGAAGTCGACGACATGGTCGAGGGCCTGTCGAGCC
TGATGGAGCCGATCATCATCGTGTTCCTCGGTGGCCTGATCGGCGGTATCGTGGTATCCATGTACCTGCCCATCTTCAAG
CTCGGCTCCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127JQF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.198

99.753

0.551
  pilG Neisseria gonorrhoeae MS11

52.618

99.012

0.521
  pilG Neisseria meningitidis 44/76-A

52.369

99.012

0.519
  pilC Acinetobacter baylyi ADP1

52.513

98.272

0.516
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilC Acinetobacter baumannii D1279779

51.385

98.025

0.504
  pilC Vibrio cholerae strain A1552

41.814

98.025

0.41
  pilC Vibrio campbellii strain DS40M4

40.399

99.012

0.4
  pilC Thermus thermophilus HB27

39.348

98.519

0.388

Multiple sequence alignment