Detailed information NTG0140848

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: UC35_RS03720
• Genome accession: NZ_CP010951
• Coordinates: 763428..764270 (+)
• Length: 280 a.a.
• NCBI ID: WP_061496308.1
• Uniprot ID: A0A127JQ75
• Organism: Ramlibacter tataouinensis strain 5-10
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 758428..769270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
UC35_RS03705 (UC35_03700)	-	758892..760292 (+)	1401	WP_061496302.1	glycosyltransferase	-
UC35_RS03710 (UC35_03705)	pilB	760437..762176 (+)	1740	WP_061496304.1	type IV-A pilus assembly ATPase PilB	Machinery gene
UC35_RS03715 (UC35_03710)	pilC	762211..763428 (+)	1218	WP_061496306.1	type II secretion system F family protein	Machinery gene
UC35_RS03720 (UC35_03715)	pilD	763428..764270 (+)	843	WP_061496308.1	prepilin peptidase	Machinery gene
UC35_RS03725 (UC35_03720)	coaE	764273..764878 (+)	606	WP_061496310.1	dephospho-CoA kinase	-
UC35_RS03730 (UC35_03725)	zapD	764886..765641 (+)	756	WP_061496312.1	cell division protein ZapD	-
UC35_RS03735 (UC35_03730)	-	765641..765853 (+)	213	WP_061496314.1	DNA gyrase inhibitor YacG	-
UC35_RS03740 (UC35_03735)	-	765854..766321 (-)	468	WP_061496316.1	NUDIX domain-containing protein	-
UC35_RS03745 (UC35_03740)	-	766318..767184 (-)	867	WP_061496319.1	ATP-binding protein	-
UC35_RS03750 (UC35_03745)	argJ	767181..768407 (-)	1227	WP_061496320.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
UC35_RS24155	-	768454..768888 (-)	435	WP_227820542.1	rhodanese-like domain-containing protein	-

Sequence

Protein

• Length: 280 a.a.
• Molecular weight: 30346.99 Da
• Isoelectric Point: 8.1618

>NTDB_id=140848 UC35_RS03720 WP_061496308.1 763428..764270(+) (pilD) [Ramlibacter tataouinensis strain 5-10]
MLDPSLLHAGLAGVFGLLVGSFLNVVIYRLPKMMERQWAAECAELAGHAPPAAEEFNLMRPRSRCRQCGHQIRWWENIPV
ASYLALRGKCASCGTPIGLRYPLVELATGALFYFCVRHWGPTTEGAAWCLFSAAIVALALIDWDTTLLPDDITLPLLWAG
LVFAALRWTPLPLSHAVWGAVGGYLSLWLVYHAFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILMASVIGAIIGIL
MKIFSRLREGGYVPFGPFLAAAGLTAMVFGPAAILQAVGL

Nucleotide

• Length: 843 bp

>NTDB_id=140848 UC35_RS03720 WP_061496308.1 763428..764270(+) (pilD) [Ramlibacter tataouinensis strain 5-10]
ATGCTCGACCCCTCCCTGCTGCACGCCGGCCTGGCCGGCGTGTTCGGCCTGCTCGTCGGAAGCTTCCTGAACGTCGTCAT
CTACCGCTTGCCGAAGATGATGGAGCGGCAGTGGGCGGCCGAGTGCGCCGAACTGGCGGGCCACGCCCCGCCGGCAGCCG
AGGAATTCAACCTGATGCGGCCGCGCTCGCGCTGCCGCCAATGCGGCCACCAGATTCGCTGGTGGGAAAACATCCCCGTG
GCCAGCTACTTGGCGCTGCGCGGCAAGTGCGCCAGCTGCGGCACCCCGATCGGCCTGCGCTATCCCCTGGTCGAACTCGC
GACCGGCGCGCTGTTCTACTTCTGCGTCCGCCACTGGGGGCCCACGACCGAAGGCGCGGCCTGGTGCCTGTTCTCGGCTG
CCATCGTCGCACTGGCGCTGATCGATTGGGACACCACCCTCCTGCCGGACGACATCACCCTGCCCCTCTTGTGGGCGGGC
CTCGTGTTCGCCGCCCTGCGCTGGACGCCGCTGCCCCTGTCGCATGCGGTCTGGGGCGCGGTCGGCGGCTACCTCTCGCT
CTGGCTGGTCTACCACGCCTTCAAGCTGGCGACCGGCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTGTTCGCCGCGC
TTGGCGCATGGTTCGGCTGGCAGGCGCTGGTTCCCATCATCCTGATGGCGTCCGTGATCGGCGCGATCATCGGCATCCTG
ATGAAGATCTTCAGCCGCCTGCGCGAGGGCGGCTATGTGCCCTTCGGGCCCTTTCTCGCGGCCGCCGGCCTGACCGCCAT
GGTGTTCGGCCCGGCCGCCATCCTGCAGGCCGTAGGACTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-118)

• Type IV leader peptidase family (Peptidase_A24) (131-239)

3D structure

• AlphaFold DB: A0A127JQ75

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.301	96.071	0.493
pilD	Vibrio cholerae strain A1552	51.685	95.357	0.493
pilD	Neisseria gonorrhoeae MS11	50.781	91.429	0.464
pilD	Acinetobacter baumannii D1279779	47.674	92.143	0.439
pilD	Acinetobacter nosocomialis M2	46.899	92.143	0.432