Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   UC35_RS03710 Genome accession   NZ_CP010951
Coordinates   760437..762176 (+) Length   579 a.a.
NCBI ID   WP_061496304.1    Uniprot ID   A0A127JQ45
Organism   Ramlibacter tataouinensis strain 5-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 755437..767176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC35_RS03695 - 755483..757288 (+) 1806 WP_061496298.1 acetate--CoA ligase family protein -
  UC35_RS03700 (UC35_03695) - 757285..758895 (+) 1611 WP_158513854.1 alkaline phosphatase family protein -
  UC35_RS03705 (UC35_03700) - 758892..760292 (+) 1401 WP_061496302.1 glycosyltransferase -
  UC35_RS03710 (UC35_03705) pilB 760437..762176 (+) 1740 WP_061496304.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UC35_RS03715 (UC35_03710) pilC 762211..763428 (+) 1218 WP_061496306.1 type II secretion system F family protein Machinery gene
  UC35_RS03720 (UC35_03715) pilD 763428..764270 (+) 843 WP_061496308.1 prepilin peptidase Machinery gene
  UC35_RS03725 (UC35_03720) coaE 764273..764878 (+) 606 WP_061496310.1 dephospho-CoA kinase -
  UC35_RS03730 (UC35_03725) zapD 764886..765641 (+) 756 WP_061496312.1 cell division protein ZapD -
  UC35_RS03735 (UC35_03730) - 765641..765853 (+) 213 WP_061496314.1 DNA gyrase inhibitor YacG -
  UC35_RS03740 (UC35_03735) - 765854..766321 (-) 468 WP_061496316.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62846.08 Da        Isoelectric Point: 5.0709

>NTDB_id=140846 UC35_RS03710 WP_061496304.1 760437..762176(+) (pilB) [Ramlibacter tataouinensis strain 5-10]
MAAADSVLAADSPSVALPGLARALISAGKLEQKTAEDVSRKAQGNRTSFIAELVGSGAVSATDLAHTMSGAFGAPLLDLE
AVDPQRLPKNLLDSKMCQALRVLVLAKRNNRLIVATADPSDQQAAEKIKFSTQMGVDWIITEYDKLTKMIEASATSASEV
MDSIIGDDFEFDESTMDSESADEGDQTPSEVEDAPVVKFLHKMLVDAFTMRASDLHFEPYEHTYRVRFRIDGELREIASP
PTAIKEKLASRIKVISKMDISEKRVPQDGRMKLKIGPERVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEVEEK
TRLLEAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGINISTAEDPSEINLPGVNQVNMNDKAGLTFAAALKSFLRQD
PDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPHCKAPADI
PHETLLEAGFKDEDVDGTWTPYKPVGCSMCNNGYKGRVGIYQVMPISEDMQRIILADGSAMDIAKQSESEGVKSLRQSGL
NKVKLGVTSLEEVLGCTNL

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=140846 UC35_RS03710 WP_061496304.1 760437..762176(+) (pilB) [Ramlibacter tataouinensis strain 5-10]
ATGGCCGCCGCCGATTCCGTCCTAGCCGCTGATTCGCCTTCCGTCGCGCTGCCGGGCCTGGCCCGGGCGCTCATTTCAGC
CGGCAAGCTGGAACAAAAAACGGCCGAGGACGTCTCGCGCAAGGCCCAGGGCAACCGAACCAGTTTCATTGCCGAGCTCG
TCGGCTCCGGCGCGGTGTCGGCCACCGACCTGGCGCACACCATGTCCGGCGCGTTCGGCGCGCCCCTGCTGGACCTGGAA
GCCGTCGACCCGCAGCGTCTGCCCAAGAACCTGCTGGACTCCAAGATGTGCCAGGCGCTGCGCGTGCTGGTGCTGGCCAA
GCGCAACAACCGCCTGATCGTGGCCACGGCCGATCCCAGCGACCAGCAGGCGGCAGAGAAGATCAAGTTCTCCACGCAGA
TGGGCGTGGACTGGATCATCACGGAGTACGACAAGCTCACCAAGATGATCGAGGCCAGCGCCACTTCGGCGAGCGAGGTC
ATGGACAGCATCATCGGCGACGACTTCGAGTTCGACGAGTCGACGATGGACTCCGAGAGCGCCGACGAGGGCGACCAGAC
CCCTTCCGAGGTCGAGGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGGTGGACGCGTTCACCATGCGCGCATCCG
ACCTGCACTTCGAGCCCTACGAGCACACCTACCGGGTTCGCTTCCGGATCGACGGCGAGCTGCGCGAGATCGCCTCGCCG
CCGACCGCCATCAAGGAAAAGCTCGCTTCGCGCATCAAGGTGATCTCCAAGATGGACATCTCCGAGAAGCGCGTGCCGCA
GGACGGCCGGATGAAGCTCAAGATCGGGCCGGAGCGGGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGTATCCTGGACCCGAGCAGCGCCAAGCTGGGGATCGACGCGCTCGGCTACGAGGTCGAGGAGAAG
ACTCGCCTGCTCGAGGCCATCAACCGGCCCTACGGGATGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTGTC
GCTCTACACCTGCCTGAACCTGCTGAACAAGCCGGGCATCAACATCTCGACGGCGGAAGACCCGTCGGAAATCAACCTGC
CCGGCGTCAACCAGGTGAACATGAACGACAAGGCCGGCCTGACCTTCGCCGCCGCGCTGAAGTCCTTCCTGCGCCAGGAC
CCGGACGTGATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCGGCGCAGACCGGCCACCT
GGTGCTGTCGACCCTGCACACCAACGACGCGCCGACCACGCTCACGCGGATGCGCAACATGGGCATCGCGCCGTTCAACA
TCGCCTCCAGCGTGATCCTGATCACCGCGCAGCGACTGGCGCGGCGGCTGTGCCCCCACTGCAAGGCGCCGGCCGACATC
CCCCACGAGACGCTGCTGGAAGCCGGCTTCAAGGACGAAGACGTGGACGGCACCTGGACCCCCTACAAGCCGGTGGGCTG
CTCCATGTGCAACAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCAGCGAGGACATGCAGCGCATCA
TCCTGGCCGACGGCAGCGCCATGGACATTGCCAAGCAGTCGGAGTCCGAAGGCGTCAAGAGCCTGCGGCAATCCGGCTTG
AACAAGGTCAAGCTCGGAGTCACCTCGCTGGAAGAGGTCTTGGGCTGCACCAACCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127JQ45

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.985

93.782

0.525
  pilB Acinetobacter baylyi ADP1

53.915

97.064

0.523
  pilF Neisseria gonorrhoeae MS11

50.796

97.582

0.496
  pilB Legionella pneumophila strain ERS1305867

50.446

96.891

0.489
  pilB Vibrio cholerae strain A1552

45.699

96.373

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.586

95.855

0.437
  pilB Vibrio campbellii strain DS40M4

44.464

98.273

0.437
  pilF Thermus thermophilus HB27

37.876

97.582

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

91.364

0.361

Multiple sequence alignment