Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Achr_RS09485 Genome accession   NZ_CP010415
Coordinates   2088813..2089970 (+) Length   385 a.a.
NCBI ID   WP_052263897.1    Uniprot ID   A0A0C4WM24
Organism   Azotobacter chroococcum NCIMB 8003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2083813..2094970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS09470 (Achr_18820) - 2084402..2086975 (+) 2574 WP_052263896.1 glycoside hydrolase family 32 protein -
  Achr_RS09475 (Achr_18830) - 2087041..2088242 (-) 1202 Protein_1929 MFS transporter -
  Achr_RS09480 (Achr_18850) - 2088413..2088691 (-) 279 WP_039803822.1 peptidylprolyl isomerase -
  Achr_RS09485 (Achr_18860) pilU 2088813..2089970 (+) 1158 WP_052263897.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Achr_RS09490 (Achr_18870) - 2090422..2091738 (+) 1317 WP_039803824.1 RNA-guided endonuclease InsQ/TnpB family protein -
  Achr_RS09495 (Achr_18880) - 2091745..2093124 (-) 1380 WP_039803826.1 substrate-binding domain-containing protein -
  Achr_RS09500 (Achr_18890) xthA 2093258..2094070 (-) 813 WP_039803827.1 exodeoxyribonuclease III -
  Achr_RS09505 (Achr_18900) - 2094206..2094844 (+) 639 WP_082045402.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42616.74 Da        Isoelectric Point: 6.3262

>NTDB_id=136984 Achr_RS09485 WP_052263897.1 2088813..2089970(+) (pilU) [Azotobacter chroococcum NCIMB 8003]
MDIQSMLRVLATQDGSDLYLSTGAPPCAKFNGVLKALGQEPLQPGAVAKIAEGLMDAEQRVEFERELEMNLAISLPNIGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPKILLDVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLSNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTVRDLIKRSELSEIKEIMEKSRNLGMQTFDQAL
IELVNEGAIGEEEALKNADSANNVRLKLKLHRDGGVAGADKQGGESPWQGLSLEMVEEPEEAGNS

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=136984 Achr_RS09485 WP_052263897.1 2088813..2089970(+) (pilU) [Azotobacter chroococcum NCIMB 8003]
ATGGATATTCAATCGATGCTGAGAGTCCTGGCCACCCAGGACGGTTCGGACCTTTACCTGTCCACCGGCGCGCCGCCCTG
CGCGAAGTTCAACGGCGTGCTCAAGGCGCTCGGCCAGGAGCCGCTGCAGCCGGGCGCCGTGGCAAAGATCGCCGAGGGGC
TGATGGATGCCGAGCAGCGCGTCGAGTTCGAGCGCGAGCTGGAAATGAACCTGGCGATCTCCCTGCCGAACATCGGCCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATTCCGCGCTTCGA
GGACCTCAAGCTGCCGAAGATCCTCCTCGACGTGGTGATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAT
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGCGTGGACACCAGAAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCAATTTCCACGCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAACTGCTCAACGACCTGAGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTCAAGACGGTCGACGGCAAGCGCCGGGCCGCGGTGGAGGTGCTGCTCGGCACGCCGACGGTGCGCGACCTGATCA
AGCGCAGCGAGCTGTCCGAGATCAAGGAGATCATGGAGAAGTCACGCAACCTCGGCATGCAGACCTTCGACCAGGCGCTG
ATCGAGCTGGTCAACGAGGGCGCCATCGGCGAGGAGGAGGCGCTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTGAAGCTGCACCGCGACGGCGGGGTAGCCGGCGCCGACAAGCAAGGCGGGGAAAGCCCTTGGCAGGGGCTGAGCCTGG
AGATGGTCGAGGAGCCGGAGGAGGCCGGCAACAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4WM24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.881

98.442

0.54
  pilU Acinetobacter baylyi ADP1

52.273

91.429

0.478
  pilU Vibrio cholerae strain A1552

51.841

91.688

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.068

88.052

0.379
  pilT Legionella pneumophila strain Lp02

42.814

86.753

0.371
  pilT Legionella pneumophila strain ERS1305867

42.814

86.753

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

86.494

0.364
  pilT Vibrio cholerae strain A1552

42.042

86.494

0.364
  pilT Pseudomonas aeruginosa PAK

40.816

89.091

0.364
  pilT Neisseria meningitidis 8013

40.058

90.13

0.361
  pilT Neisseria gonorrhoeae MS11

40.058

90.13

0.361

Multiple sequence alignment