Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   AO353_RS14335 Genome accession   NZ_CP012830
Coordinates   3177449..3177859 (+) Length   136 a.a.
NCBI ID   WP_054595548.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172449..3182859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS14310 (AO353_14310) yacG 3172609..3172809 (-) 201 WP_054595543.1 DNA gyrase inhibitor YacG -
  AO353_RS14315 (AO353_14315) coaE 3172806..3173429 (-) 624 WP_054595544.1 dephospho-CoA kinase -
  AO353_RS14320 (AO353_14320) pilD 3173426..3174298 (-) 873 WP_054595545.1 prepilin peptidase Machinery gene
  AO353_RS14325 (AO353_14325) pilC 3174301..3175518 (-) 1218 WP_054595546.1 type II secretion system F family protein Machinery gene
  AO353_RS14330 (AO353_14330) pilB 3175522..3177222 (-) 1701 WP_054595547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO353_RS14335 (AO353_14335) comP 3177449..3177859 (+) 411 WP_054595548.1 pilin Machinery gene
  AO353_RS14340 (AO353_14340) - 3178137..3178493 (+) 357 WP_054595549.1 BON domain-containing protein -
  AO353_RS14345 (AO353_14345) - 3178605..3178904 (-) 300 WP_054595550.1 DUF2845 domain-containing protein -
  AO353_RS14350 (AO353_14350) - 3178907..3181849 (-) 2943 WP_054595551.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13788.05 Da        Isoelectric Point: 8.1087

>NTDB_id=136642 AO353_RS14335 WP_054595548.1 3177449..3177859(+) (comP) [Pseudomonas fluorescens strain FW300-N2E3]
MNNQKGFTLIELLIVVAIIGILATFALPLYSKYQARAKVTAGLAEISALKVPFEDIINQGTDPTLALIGGTATTGNCTIT
ASGTASSGAGTIVCTIVNAPGPVLSKTITLTRTLAGGWTCATTALQDYAPKGCTGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=136642 AO353_RS14335 WP_054595548.1 3177449..3177859(+) (comP) [Pseudomonas fluorescens strain FW300-N2E3]
ATGAATAATCAAAAAGGTTTTACCCTCATCGAGCTGCTGATCGTGGTGGCGATCATCGGCATTCTGGCGACGTTCGCGTT
GCCGCTGTATTCCAAGTATCAGGCGCGGGCGAAGGTCACCGCGGGGCTGGCGGAAATTTCAGCACTGAAGGTGCCTTTTG
AAGACATTATCAATCAGGGGACCGACCCGACACTGGCGCTGATCGGTGGTACGGCAACTACCGGTAACTGCACAATCACG
GCATCGGGTACTGCTTCCAGCGGTGCAGGTACTATTGTTTGCACGATTGTCAATGCACCAGGTCCGGTGCTCAGCAAAAC
CATCACGCTGACGCGGACACTGGCCGGTGGCTGGACCTGCGCGACCACCGCGCTGCAGGACTACGCGCCCAAGGGTTGCA
CTGGCGTCTGA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

41.667

100

0.478
  pilA Acinetobacter baumannii strain A118

47.101

100

0.478
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.715

100

0.471
  pilA2 Legionella pneumophila str. Paris

41.667

100

0.441
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

99.265

0.441
  pilA2 Legionella pneumophila strain ERS1305867

40.972

100

0.434
  pilA Vibrio cholerae C6706

41.549

100

0.434
  pilA Vibrio cholerae strain A1552

41.549

100

0.434
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.549

100

0.434
  pilA Pseudomonas aeruginosa PAK

39.597

100

0.434