Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   BT4G5_RS11965 Genome accession   NZ_CP010089
Coordinates   2279785..2280117 (+) Length   110 a.a.
NCBI ID   WP_001082769.1    Uniprot ID   A0A9W5QHQ8
Organism   Bacillus thuringiensis serovar galleriae strain 4G5     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2258389..2282011 2279785..2280117 within 0


Gene organization within MGE regions


Location: 2258389..2282011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BT4G5_RS11920 (BT4G5_11920) - 2259450..2262179 (+) 2730 WP_021728047.1 hypothetical protein -
  BT4G5_RS11925 (BT4G5_11925) - 2262182..2263933 (+) 1752 WP_021729424.1 phage integrase -
  BT4G5_RS11930 (BT4G5_11930) - 2263926..2264642 (+) 717 WP_021728389.1 hypothetical protein -
  BT4G5_RS11935 (BT4G5_11935) - 2265403..2269125 (+) 3723 WP_042991544.1 ATP-binding protein -
  BT4G5_RS11940 (BT4G5_11940) - 2269580..2270947 (-) 1368 WP_021728391.1 hypothetical protein -
  BT4G5_RS11945 (BT4G5_11945) - 2271125..2271352 (+) 228 WP_021728392.1 helix-turn-helix transcriptional regulator -
  BT4G5_RS11950 (BT4G5_11950) - 2271745..2271966 (+) 222 WP_021728393.1 hypothetical protein -
  BT4G5_RS11955 (BT4G5_11955) - 2272720..2272989 (-) 270 Protein_2268 GNAT family N-acetyltransferase -
  BT4G5_RS11960 (BT4G5_11960) - 2273403..2279405 (+) 6003 WP_021728395.1 ATP-binding protein -
  BT4G5_RS11965 (BT4G5_11965) ssbA 2279785..2280117 (+) 333 WP_001082769.1 single-stranded DNA-binding protein Machinery gene
  BT4G5_RS11970 (BT4G5_11970) - 2280454..2281014 (-) 561 WP_000714949.1 AAA family ATPase -

Sequence


Protein


Download         Length: 110 a.a.        Molecular weight: 12610.39 Da        Isoelectric Point: 9.4344

>NTDB_id=134161 BT4G5_RS11965 WP_001082769.1 2279785..2280117(+) (ssbA) [Bacillus thuringiensis serovar galleriae strain 4G5]
MNRVVLIGRLTKEPELYYTKQGVAYARICVAVNRGFRNSLGEQQVDFINCVVWRKSAENVAEYCKKGSLVGITGRIQTSN
YDDEQGKRIYRTEVVIESITFLERRREGAS

Nucleotide


Download         Length: 333 bp        

>NTDB_id=134161 BT4G5_RS11965 WP_001082769.1 2279785..2280117(+) (ssbA) [Bacillus thuringiensis serovar galleriae strain 4G5]
TTGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAACAAGGCGTCGCTTATGCACG
AATATGTGTTGCGGTGAATAGAGGATTTCGAAATAGTTTAGGTGAACAACAAGTCGATTTTATTAATTGTGTCGTTTGGC
GCAAATCGGCTGAGAATGTAGCTGAATATTGTAAGAAGGGGTCGCTCGTTGGGATTACAGGGCGTATTCAGACTAGTAAT
TACGATGATGAACAAGGCAAGAGAATATATAGAACTGAAGTTGTGATTGAGAGTATTACCTTTTTGGAGAGAAGGCGGGA
GGGGGCATCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

59.048

95.455

0.564

  ssb Latilactobacillus sakei subsp. sakei 23K

56.19

95.455

0.536

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.351

100

0.518

  ssbB Lactococcus lactis subsp. cremoris KW2

45.714

95.455

0.436

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.519

98.182

0.427

  ssbA Streptococcus mutans UA159

40.741

98.182

0.4

  ssbB/cilA Streptococcus mitis NCTC 12261

40.741

98.182

0.4

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.741

98.182

0.4

  ssbB/cilA Streptococcus pneumoniae D39

39.815

98.182

0.391

  ssbB/cilA Streptococcus pneumoniae R6

39.815

98.182

0.391

  ssbB/cilA Streptococcus mitis SK321

39.815

98.182

0.391

  ssbB/cilA Streptococcus pneumoniae Rx1

39.815

98.182

0.391


Multiple sequence alignment