Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PN96_RS15060 Genome accession   NZ_CP009977
Coordinates   3224521..3226260 (+) Length   579 a.a.
NCBI ID   WP_024372659.1    Uniprot ID   A0AAN1CXH1
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3219521..3231260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN96_RS15040 (PN96_14830) pilM 3221823..3222839 (+) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  PN96_RS15045 (PN96_14835) pilN 3222823..3223398 (+) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  PN96_RS15050 (PN96_14840) pilO 3223391..3223984 (+) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  PN96_RS15055 (PN96_14845) pilP 3223974..3224489 (+) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  PN96_RS15060 (PN96_14850) pilQ 3224521..3226260 (+) 1740 WP_024372659.1 type IV pilus secretin PilQ family protein Machinery gene
  PN96_RS15065 (PN96_14855) aroK 3226449..3226967 (+) 519 WP_014233147.1 shikimate kinase AroK -
  PN96_RS15070 (PN96_14860) aroB 3226999..3228099 (+) 1101 WP_020334612.1 3-dehydroquinate synthase -
  PN96_RS15075 (PN96_14865) - 3228152..3229645 (+) 1494 WP_020334611.1 AAA family ATPase -
  PN96_RS15080 (PN96_14870) - 3229715..3230554 (+) 840 WP_020334610.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63509.47 Da        Isoelectric Point: 4.3958

>NTDB_id=133349 PN96_RS15060 WP_024372659.1 3224521..3226260(+) (pilQ) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MIMRQGLTRTPRMLTGLLSFWLVLLFTPFALADGGMSNALKSIDFRTNKDKDAVIVVELSSPAAIVDIQRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVDSVEVFREVSTTRLVAHISDDYTHDYRLAGRYLEITVSKLKSDEKTPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQQLEEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLVRELPENILVIRDIIESLDIPVK
QVQIEARIVTVSEGNMDELGIRWGFSSINGNNTVGGSIENNLATIGLYEGDGEEGEGGSVGIDDFLNVNLAATNPSATSI
AFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTITFKKAVLSLKVTPQITP
DNRLVLDLSVTQDRPGQVVKTGTGEAVTINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRSY
EKVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=133349 PN96_RS15060 WP_024372659.1 3224521..3226260(+) (pilQ) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGATAATGAGACAAGGACTTACACGTACACCAAGGATGCTCACTGGGCTCTTATCCTTTTGGTTAGTTTTACTTTTCAC
CCCTTTTGCTTTGGCTGATGGCGGGATGTCTAATGCGTTAAAGAGTATCGACTTTCGCACGAACAAGGACAAAGACGCGG
TGATTGTGGTGGAACTGTCTTCACCGGCTGCGATTGTCGATATTCAGCGGGTTCAGGAAGGACTGAGCATCGACTTGCTT
AATACGTCAGTGAAAGATGAGCAGTTATATCTCCTCGATGTAAAAGACTTTTCAACGGTAGTGGACAGCGTGGAAGTCTT
TCGTGAAGTCTCGACGACCCGTTTAGTCGCGCATATTAGTGATGATTACACCCATGATTACCGTTTGGCAGGGCGCTATT
TGGAAATCACGGTCAGTAAACTCAAGTCTGATGAAAAAACACCAGATAAGAGCATTTTAGAGAAAGAAGGCAAACTGATA
TCGATTAACTTCCAGGATATCCCGGTACGTAACGTCTTACAGCTCATTGCCGACTATAACCAGTTTAACCTGGTGGTATC
AGACTCGGTCGAAGGCAACCTGACACTGCGACTTGATGGTGTGCCTTGGCAGCAGGTTTTAGACATTATTCTGCAAGTAA
AAGGTTTGGATAAGCGAGTTGATGGTAATGTCATTCTTGTCGCGCCGACGGATGAACTTGACCTTCGAGAGAAGCAGCAA
CTAGAGAAGCAGCAACTTGAAGAGGAAATGGGCGAACTGTCGTCTGAAATCATCAAAGTGAATTTTGCCAAAGCATCCGA
TATTGCTGAGATGATTAATGGTGAAGGCAATATCAGCATGCTCTCTGAGCGAGGCAGCATGACGATTGATGAGCGCACTA
ACTCACTATTGGTCAGAGAATTGCCAGAGAATATCTTGGTTATTCGTGACATTATTGAGTCACTCGATATTCCGGTTAAG
CAGGTCCAAATCGAAGCGAGGATTGTCACCGTCAGTGAAGGGAACATGGATGAACTCGGCATTCGATGGGGATTCTCTTC
CATCAACGGCAATAACACCGTTGGTGGCTCGATTGAGAATAACCTCGCAACCATTGGCCTTTATGAAGGTGATGGTGAAG
AGGGCGAGGGCGGTAGTGTTGGAATTGATGATTTTCTTAACGTCAACCTGGCCGCTACTAACCCGAGCGCAACCAGCATT
GCGTTTCAGGTCGCCAAGTTGGGCTCTGACACTTTGCTCGATCTGGAACTTTCTGCACTGCAGCAGGAGTCGAAAGCTGA
GATCATCTCAAGCCCTCGTTTAATCACTACTAATAAAAAACCGGCCTATATAGAACAAGGTACAGAAATTCCGTATCTAG
AATCATCATCGAGTGGTGCGACAACCATTACGTTCAAAAAAGCTGTACTAAGTCTGAAAGTCACGCCACAAATTACCCCG
GATAACCGTCTGGTGCTGGATTTAAGTGTTACTCAGGACAGACCGGGGCAAGTGGTAAAAACGGGAACTGGCGAAGCGGT
CACAATAAACACCCAGAGAATAGGTACTCAAGTCTTAGTAAACAACGGTGAGACGGTTGTTCTTGGCGGCATTTTCCAAC
ACAGCATTACCAACTCTGTCGATAAAGTGCCATTACTCGGTGATCTGCCGTTATTAGGTGCACTTTTCCGCAGAAGTTAC
GAAAAAGTTGGCAAAAGTGAACTGCTGATTTTCGTTACACCAAAGGTTGTTATTCAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(419-577)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

88.793

100

0.889
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

75.752

97.582

0.739
  pilQ Vibrio cholerae strain A1552

75.752

97.582

0.739
  pilQ Legionella pneumophila strain ERS1305867

41.091

94.991

0.39
  pilQ Legionella pneumophila strain Lp02

41.091

94.991

0.39
  pilQ Pseudomonas aeruginosa PAK

40.399

95.337

0.385
  pilQ Acinetobacter baumannii strain A118

37.99

100

0.385
  pilQ Acinetobacter baumannii D1279779

37.99

100

0.385
  comQ Acinetobacter baylyi ADP1

37.651

100

0.377

Multiple sequence alignment